浮遊培養、懸濁培養、懸濁培養液

WordNet

grow in a special preparation; "the biologist grows microorganisms"
(biology) the growing of microorganisms in a nutrient medium (such as gelatin or agar); "the culture of cells in a Petri dish"
the raising of plants or animals; "the culture of oysters"
a particular society at a particular time and place; "early Mayan civilization" (同)civilization, civilisation
the attitudes and behavior that are characteristic of a particular social group or organization; "the developing drug culture"; "the reason that the agency is doomed to inaction has something to do with the FBI culture"
the tastes in art and manners that are favored by a social group
a mixture in which fine particles are suspended in a fluid where they are supported by buoyancy
the act of suspending something (hanging it from above so it moves freely); "there was a small ceremony for the hanging of the portrait" (同)dangling, hanging
a mechanical system of springs or shock absorbers connecting the wheels and axles to the chassis of a wheeled vehicle (同)suspension system
a temporary debarment (from a privilege or position etc) (同)temporary removal
an interruption in the intensity or amount of something (同)respite, reprieve, hiatus, abatement

PrepTutorEJDIC

〈U〉『教養』;修養;教化 / 〈C〉〈U〉『文化』(主として精神面をさし,生活・習慣・物の考え方などを含む)Greek cultureギリシア文化 / 〈U〉(…の)『耕作』;『栽培』,養殖,培養《+『of』+『名』》
〈U〉つるすこと,つるされた状態 / 〈U〉停学,停職;(法律・免状などの)一時停止,(判断などの)保留《+of+名》 / 〈C〉(液体の中に固体の微粒子のある)懸濁液;(液体・気体中の)浮遊物
教養のある,洗練された,上品な / 栽培(養殖)された

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1. 腹膜透析における腹膜炎の臨床症状および診断 clinical manifestations and diagnosis of peritonitis in peritoneal dialysis
2. 培養陰性感染性心内膜炎の疫学、微生物学、および診断 epidemiology microbiology and diagnosis of culture negative endocarditis
3. コクシジオイデス症の臨床検査診断 laboratory diagnosis of coccidioidomycosis
4. 女性の骨盤臓器脱：先端脱（子宮脱または膣円蓋脱）の外科的修復 pelvic organ prolapse in women surgical repair of apical prolapse uterine or vaginal vault prolapse
5. 感染性心内膜炎の臨床症状および診断 clinical manifestations and diagnosis of infective endocarditis

English Journal

Comparative study using spheres, rods and spindle-shaped nanoplatelets on dispersion stability, dissolution and toxicity of CuO nanomaterials.

Misra SK, Nuseibeh S, Dybowska A, Berhanu D, Tetley TD, Valsami-Jones E.Author information Earth & Env. Sciences, School of Geography, University of Birmingham , Birmingham , UK.AbstractCopper oxide nanoparticles with different shapes were used to examine the effect of shape on the various physicochemical properties (reactivity, aggregation, suspension stability) and to examine the behaviour by which CuO nanoparticles exhibit their biological response towards alveolar type-I cells. The different shapes examined in this study include spherical-, rod- and spindle-shaped platelet particles. In vitro dissolution studies (7 days) in 1 mM NaNO3 matrix showed a marked difference in dissolved Cu release between the nanoparticles. However, in serum-free cell-culture media (exposure media to cells), the particles' dissolution was found to be significantly enhanced with close to complete dissolution reported for all particle types. Biological studies showed both shape and size of the CuO nanoparticles tested to have a significant effect on TT-1 cell viability and release of pro-inflammatory cytokines IL-6 and IL-8. This study shows a complex interplay between particulate and dissolved species triggering the biological response. Upon immediate exposure of CuO nanoparticles of different shapes, the particulate form contributes towards the toxicity. However, for any biological response observed over and beyond a period of 24 h, the dissolved fraction becomes significant.
Nanotoxicology.Nanotoxicology.2014 Jun;8(4):422-32. doi: 10.3109/17435390.2013.796017. Epub 2013 May 15.
Copper oxide nanoparticles with different shapes were used to examine the effect of shape on the various physicochemical properties (reactivity, aggregation, suspension stability) and to examine the behaviour by which CuO nanoparticles exhibit their biological response towards alveolar type-I cells.
PMID 23590525

An injectable spheroid system with genetic modification for cell transplantation therapy.

Uchida S1, Itaka K2, Nomoto T3, Endo T4, Matsumoto Y5, Ishii T3, Kataoka K6.Author information 1Division of Clinical Biotechnology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.2Division of Clinical Biotechnology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. Electronic address: itaka-ort@umin.net.3Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan.4Department of Materials Engineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.5Department of Otorhinolaryngology and Head and Neck Surgery, Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, Tokyo, Japan.6Division of Clinical Biotechnology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan; Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan; Department of Materials Engineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. Electronic address: kataoka@bmw.t.u-tokyo.ac.jp.AbstractThe new methodology to increase a therapeutic potential of cell transplantation was developed here by the use of three-dimensional spheroids of transplanting cells subsequent to the genetic modification with non-viral DNA vectors, polyplex nanomicelles. Particularly, spheroids in regulated size of 100-μm of primary hepatocytes transfected with luciferase gene were formed on the micropatterned culture plates coated with thermosensitive polymer, and were recovered in the form of injectable liquid suspension simply by cooling the plates. After subcutaneously transplanting these hepatocyte spheroids, efficient transgene expression was observed in host tissue for more than a month, whereas transplantation of a single-cell suspension from a monolayer culture resulted in an only transient expression. The spheroid system contributed to the preservation of innate functions of transplanted hepatocytes in the host tissue, such as albumin expression, thereby possessing high potential for expressing transgene. Intravital observation of transplanted cells showed that those from spheroid cultures had a tendency to localize in the vicinity of blood vessels, making a favorable microenvironment for preserving cell functionality. Furthermore, spheroids transfected with erythropoietin-expressing DNA showed a significantly higher hematopoietic effect than that of cell suspensions from monolayer cultures, demonstrating high potential of this genetically-modified spheroid transplantation system for therapeutic applications.
Biomaterials.Biomaterials.2014 Mar;35(8):2499-506. doi: 10.1016/j.biomaterials.2013.12.012. Epub 2013 Dec 31.
The new methodology to increase a therapeutic potential of cell transplantation was developed here by the use of three-dimensional spheroids of transplanting cells subsequent to the genetic modification with non-viral DNA vectors, polyplex nanomicelles. Particularly, spheroids in regulated size of 1
PMID 24388386

Induction of phytic acid synthesis by abscisic acid in suspension-cultured cells of rice.

Matsuno K1, Fujimura T2.Author information 1Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan. Electronic address: s1130248@u.tsukuba.ac.jp.2Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan.AbstractA pathway of phytic acid (PA) synthesis in plants has been revealed via investigations of low phytic acid mutants. However, the regulation of this pathway is not well understood because it is difficult to control the environments of cells in the seeds, where PA is mainly synthesized. We modified a rice suspension culture system in order to study the regulation of PA synthesis. Rice cells cultured with abscisic acid (ABA) accumulate PA at higher levels than cells cultured without ABA, and PA accumulation levels increase with ABA concentration. On the other hand, higher concentrations of sucrose or inorganic phosphorus do not affect PA accumulation. Mutations in the genes RINO1, OsMIK, OsIPK1 and OsLPA1 have each been reported to confer low phytic acid phenotypes in seeds. Each of these genes is upregulated in cells cultured with ABA. OsITPK4 and OsITPK6 are upregulated in cells cultured with ABA and in developing seeds. These results suggest that the regulation of PA synthesis is similar between developing seeds and cells in this suspension culture system. This system will be a powerful tool for elucidating the regulation of PA synthesis.
Plant science : an international journal of experimental plant biology.Plant Sci.2014 Mar;217-218:152-7. doi: 10.1016/j.plantsci.2013.12.015. Epub 2014 Jan 4.
A pathway of phytic acid (PA) synthesis in plants has been revealed via investigations of low phytic acid mutants. However, the regulation of this pathway is not well understood because it is difficult to control the environments of cells in the seeds, where PA is mainly synthesized. We modified a r
PMID 24467907