WordNet

impervious to being affected; "resistant to the effects of heat"; "resistant to persuasion"
an antibiotic obtained from an actinomycete and used (as a sulphate under the trade name Neobiotic) as an intestinal antiseptic in surgery (同)fradicin, Neobiotic

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Puromycin resistance gene as an effective selection marker for ciliate Tetrahymena.

Iwamoto M1, Mori C1, Hiraoka Y2, Haraguchi T3.
Gene.Gene.2014 Jan 25;534(2):249-55. doi: 10.1016/j.gene.2013.10.049. Epub 2013 Nov 1.
A puromycin-N-acetyltransferase gene (pac) is widely used as a selection marker for eukaryotic gene manipulation. However, it has never been utilized for molecular studies in the ciliate Tetrahymena thermophila, in spite of the limited number of selection markers available for this organism. To util
PMID 24185080

Laboratory techniques to obtain different forms of Trypanosoma cruzi: applications to wild-type and genetically modified parasites.

de Cámara Mde L, Bouvier LA, Miranda MR, Reigada C, Pereira CA.
Folia parasitologica.Folia Parasitol (Praha).2013 Nov;60(5):406-10.
Nowadays, there are no simple techniques for mimicking in vitro the life cycle of the kinetoplasmtid Trypanosoma cruzi Chagas, 1909, causative agent of Chagas disease, especially for parasite strains maintained as epimastigotes for many years. In the present study, we propose a method for obtaining
PMID 24471281

Evaluating the influence of selection markers on obtaining selected pools and stable cell lines in human cells.

Lanza AM1, Kim do S, Alper HS.
Biotechnology journal.Biotechnol J.2013 Jul;8(7):811-21. doi: 10.1002/biot.201200364. Epub 2013 Apr 11.
Selection markers are common genetic elements used in recombinant cell line development. While several selection systems exist for use in mammalian cell lines, no previous study has comprehensively evaluated their performance in the isolation of recombinant populations and cell lines. Here we examin
PMID 23450727

Tumor Enhancement Effect of Overexpressed Manganese-superoxide Dismutase in Manganese-enhanced MR Imaging

HASEGAWA Sumitaka,SAITO Shigeyoshi,KOSHIKAWA YANO Michiko,FURUKAWA Takako,AOKI Ichio,SAGA Tsuneo
Magnetic resonance in medical sciences : MRMS 10(3), 155-158, 2011-09-01
… Methods: We subcutaneously implanted SK-OV-3 human ovarian cancer cells stably overexpressing Mn-SOD (SK-Mn-SOD) into athymic nude mice and SK-OV-3 cells with plasmid DNAs carrying neomycin-resistant genes (SK-neo) into the same mice for controls. …
NAID 10029698456

Establishment of a Human Hepatoma Cell Line HepG2-A10 for a Reporter Gene Assay of Arylhydrocarbon Receptor Activators

Sekimoto Masashi,Kawamagari Hiroto,Nakatani Saki [他],Nemoto Kiyomitsu,Degawa Masakuni
環境変異原研究 = Genes and environment : the official journal of the Japanese Environmental Mutagen Society 29(1), 11-16, 2007-02-20
… To establish a human hepatic cell line for a convenient reporter gene assay of arylhydrocarbon receptor (AhR) activators, the chimera plasmid containing xenobiotic responsible element (XRE), minimal SV40 promoter, and luciferase reporter gene and the expression vector pRC/CMV containing a neomycin-resistant gene were co-transfected into a human hepatoma cell line, HepG2. … Then, antibiotic (G418)-resistant HepG2 cells were selected and cloned. …
NAID 110006240238

Establishment of a Human Hepatoma Cell Line HepG2-A10 for a Reporter Gene Assay of Arylhydrocarbon Receptor Activators

Sekimoto Masashi,Kawamagari Hiroto,Nakatani Saki,Nemoto Kiyomitsu,Degawa Masakuni
Genes and Environment 29(1), 11-16, 2007
… To establish a human hepatic cell line for a convenient reporter gene assay of arylhydrocarbon receptor (AhR) activators, the chimera plasmid containing xenobiotic responsible element (XRE), minimal SV40 promoter, and luciferase reporter gene and the expression vector pRC/CMV containing a neomycin-resistant gene were co-transfected into a human hepatoma cell line, HepG2. … Then, antibiotic (G418)-resistant HepG2 cells were selected and cloned. …
NAID 130004481150