WordNet
- tributaries of the inferior mesenteric vein; drains the sigmoid colon (同)vena sigmoideus
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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2015/08/01 11:28:12」(JST)
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- Systematic improvement of lentivirus transduction protocols by antibody fragments fused to VSV-G as envelope glycoprotein.
- Höfig I1, Barth S2, Salomon M3, Jagusch V3, Atkinson MJ1, Anastasov N1, Thirion C4.Author information 1Institute of Radiation Biology, Helmholtz Center Munich, German Research Center for Environmental Health, 85764 Neuherberg, Germany.2Fraunhofer Institute for Molecular Biology and Applied Ecology IME-MB, 52074 Aachen, Germany.3Sirion Biotech GmbH, Am Klopferspitz 19, 82152 Martinsried, Germany.4Sirion Biotech GmbH, Am Klopferspitz 19, 82152 Martinsried, Germany. Electronic address: thirion@sirion-biotech.de.AbstractLentiviral vectors (LV) are widely used to successfully transduce cells for research and clinical applications. Lentiviral vectors pseudotyped with the vesicular stomatitis virus glycoprotein (VSV-G) can be produced to high titers and mediate high transduction efficiencies in vitro. For clinical applications the need for optimized transduction protocols and the limited activity of retronectin as LV enhancer, results in the application of a high multiplicity of infection (MOI) to achieve effective transduction efficiencies for a number of therapeutically relevant cells, e.g. CD34(+) hematopoietic stem cells, T- and B-cells. Our study describes an optimized LV infection protocol including a non-toxic poloxamer-based adjuvant combined with antibody-retargeted lentiviral particles, improving transduction efficiency at low MOI. Cell specificity of lentiviral vectors was increased by displaying different ratios of scFv-fused VSV-G glycoproteins on the viral envelope. The system was validated with difficult to transduce human CD30(+) lymphoma cells, and EGFR(+) tumor cells. Highly efficient transduction of lymphoma cells was achieved, >50% of cells were transduced when MOI 1 was used. The scFv displaying lentiviral particles gained relative specificity for transduction of target cells. Preferential gene delivery to CD30(+) or EGFR(+) cells was increased 4-fold in mixed cell cultures by presenting scFv antibody fragments binding to respective surface markers. A combination of spinoculation, poloxamer-based chemical adjuvant, and LV displaying scFv fragments increases transduction efficiencies of hard-to-transduce suspension lymphoma cells, and promises new chances for the future development of improved clinical protocols.
- Biomaterials.Biomaterials.2014 Apr;35(13):4204-12. doi: 10.1016/j.biomaterials.2014.01.051. Epub 2014 Feb 12.
- Lentiviral vectors (LV) are widely used to successfully transduce cells for research and clinical applications. Lentiviral vectors pseudotyped with the vesicular stomatitis virus glycoprotein (VSV-G) can be produced to high titers and mediate high transduction efficiencies in vitro. For clinical ap
- PMID 24529898
- H19 DMR methylation correlates to the progression of esophageal squamous cell carcinoma through IGF2 imprinting pathway.
- Gao T1, He B, Pan Y, Gu L, Chen L, Nie Z, Xu Y, Li R, Wang S.Author information 1Central Laboratory, Nanjing First Hospital, Nanjing Medical University, 68 Changle Road, Nanjing, 210029, Jiangsu, China, g372087144@sina.com.AbstractBACKGROUND: H19 gene has been proved to be essential for human tumor growth which contains CpG rich regions. Imprinted gene expression in many cancers is usually associated with the function of methylation. We performed this study to better understand wether H19 DMR methylation correlates to the progression of esophageal squamous cell carcinoma through IGF2 imprinting pathway.
- Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico.Clin Transl Oncol.2014 Apr;16(4):410-7. doi: 10.1007/s12094-013-1098-x. Epub 2013 Aug 13.
- BACKGROUND: H19 gene has been proved to be essential for human tumor growth which contains CpG rich regions. Imprinted gene expression in many cancers is usually associated with the function of methylation. We performed this study to better understand wether H19 DMR methylation correlates to the pro
- PMID 23943562
- Potential Usefulness of Baculovirus-mediated Sodium-iodide Symporter Reporter Gene as Non-invasively Gene Therapy Monitoring in Liver Cancer Cells: An In vitro Evaluation.
- Pan Y1, Wu H, Liu S, Zhou X, Yin H, Li B, Zhang Y.Author information 1Department of Nuclear Medicine, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, No. 197, Rui Jin 2nd Road, Shanghai 200025, China. zhang_yifan@126.com.AbstractPrimary liver cancer has one of the highest mortality rates of all cancers, and the main current treatments have a poor prognosis. This study aims to examine the efficiency of baculovirus vectors for transducing target gene into liver cancer cells and to evaluate the feasibility of using baculovirus vectors to deliver the sodium-iodide symporter (NIS) gene as a reporter gene through co-vector administration approach to monitor the expression of the target therapeutic gene in liver cancer gene therapy. We constructed (green fluorescent protein) GFP- and NIS-expressing baculovirus vectors (Bac-GFP and Bac-NIS), and measured the baculovirus transduction efficiency in HepG2 cells and other tumor cells (A549, SW1116 and 8505C), and it showed that the transduction efficiency and target gene expression level rose with increasing viral multiplicity of infection (MOI) in HepG2 cells, and HepG2 cells had a significantly higher transduction efficiency (60.8% at MOI = 200) than other tumor cells. Moreover, the baculovirus transduction was not cytotoxic to HepG2 cells at a higher MOI (MOI 5 400). We also performed dynamic iodide uptake trials, and found that Bac-NIS-transduced HepG2 cells exhibited efficient iodide uptake which could be inhibited by sodium perchlorate (NaClO4). And we measured the correlation of fluorescent intensities and 125I uptake amount in HepG2 cells after co-vector administration with Bac-NIS and Bac-GFP at different MOIs, and found a high correlation coefficient (r(2) = 0.8447), which provides a good basis for successfully evaluating the feasibility of baculovirus-mediated NIS reporter gene monitoring target gene expression in liver cancer therapy. Therefore, this study indicates that baculovirus vector is a potential vehicle for delivering therapeutic genes in studying liver cancer cells. And it is feasible to use a baculovirus vector to deliver NIS gene as a reporter gene to monitor the expression of target genes. It therefore provides an effective approach and a good basis for future baculovirus-mediated therapeutic gene delivering or therapeutic gene expression monitoring in liver cancer cells studies.
- Technology in cancer research & treatment.Technol Cancer Res Treat.2014 Apr;13(2):139-48. doi: 10.7785/tcrt.2012.500368. Epub 2013 Aug 2.
- Primary liver cancer has one of the highest mortality rates of all cancers, and the main current treatments have a poor prognosis. This study aims to examine the efficiency of baculovirus vectors for transducing target gene into liver cancer cells and to evaluate the feasibility of using baculovirus
- PMID 23919394
Japanese Journal
- 日本における大雪の経年変化とその環境場との関係 : 気象庁と鉄道の日降雪深データを用いた解析
- 鈴木 博人
- 天気 59(5), 333-350, 2012-05-31
- … 温,冬季東アジアモンスーン指標(MOI),および北極振動指標(AOI)には1980年代後半にジャンプがみられ,冬期平均気温は対象地域全てにおいてMOIおよびAOIとの相関が有意水準5%で有意である.これから,冬期平均気温のジャンプにはMOIやAOIのジャンプが関係していると考えられる.大雪の出現頻度は,多くの地域において冬期平均気温,MOI,およびAOIとの相関が有意水準5%で …
- NAID 110009457192
- Bottom shrimp trawling impacts on species distribution and fishery dynamics; Ungwana Bay fishery Kenya before and after the 2006 trawl ban
- MUNGA Cosmas,NDEGWA Stephen,FULANDA Bernerd,MANYALA Julius,KIMANI Edward,OHTOMI Jun,VANREUSEL Ann
- Fisheries science : FS 78(2), 209-219, 2012-03-01
- NAID 10030124215
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