WordNet

resistant to destruction or fading; "fast colors"
abstaining from food (同)fasting
securely fixed in place; "the post was still firm after being hit by the car" (同)firm, immobile
firmly or closely; "held fast to the rope"; "her foot was stuck fast"; "held tight" (同)tight
(of a photographic lens or emulsion) causing a shortening of exposure time; "a fast lens"
(of surfaces) conducive to rapid speeds; "a fast road"; "grass courts are faster than clay"
(used of timepieces) indicating a time ahead of or later than the correct time; "my watch is fast"
abstain from certain foods, as for religious or medical reasons; "Catholics sometimes fast during Lent"
abstain from eating; "Before the medical exam, you must fast"
acting or moving or capable of acting or moving quickly; "fast film"; "on the fast track in school"; "set a fast pace"; "a fast car"
at a rapid tempo; "the band played a fast fox trot"
quickly or rapidly (often used as a combining form); "how fast can he get here?"; "ran as fast as he could"; "needs medical help fast"; "fast-running rivers"; "fast-breaking news"; "fast-opening (or fast-closing) shutters"
used to signify the Union forces in the American Civil War (who wore blue uniforms); "a ragged blue line"
blue color or pigment; resembling the color of the clear sky in the daytime; "he had eyes of bright blue" (同)blueness
any of numerous small butterflies of the family Lycaenidae
blue clothing; "she was wearing blue"
any organization or party whose uniforms or badges are blue; "the Union army was a vast blue"
of the color intermediate between green and violet; having a color similar to that of a clear unclouded sky; "Octobers bright blue weather"- Helen Hunt Jackson; "a blue flame"; "blue haze of tobacco smoke" (同)bluish, blueish
causing dejection; "a blue day"; "the dark days of the war"; "a week of rainy depressing weather"; "a disconsolate winter landscape"; "the first dismal dispiriting days of November"; "a dark gloomy day"; "grim rainy weather" (同)dark, dingy, disconsolate, dismal, gloomy, grim, sorry, drab, drear, dreary
suggestive of sexual impropriety; "a blue movie"; "blue jokes"; "he skips asterisks and gives you the gamy details"; "a juicy scandal"; "a naughty wink"; "naughty words"; "racy anecdotes"; "a risque story"; "spicy gossip" (同)gamy, gamey, juicy, naughty, racy, risque, spicy
morally rigorous and strict; "the puritan work ethic"; "puritanic distaste for alcohol"; "she was anything but puritanical in her behavior" (同)puritanic, puritanical
turn blue
(microscopy) a dye or other coloring material that is used in microscopy to make structures visible
color for microscopic study; "The laboratory worker dyed the specimen"
color with a liquid dye or tint; "Stain this table a beautiful walnut color"; "people knew how to stain glass a beautiful blue in the middle ages"
produce or leave stains; "Red wine stains the table cloth"
a soiled or discolored appearance; "the wine left a dark stain" (同)discoloration, discolouration
a state of depression; "he had a bad case of the blues" (同)blue devils, megrims, vapors, vapours
a type of folksong that originated among Black Americans at the beginning of the 20th century; has a melancholy sound from repeated use of blue notes
marked or dyed or discolored with foreign matter; "a badly stained tablecloth"; "tear-stained cheeks"
having a coating of stain or varnish (同)varnished

PrepTutorEJDIC

『速い』,急速な,すばやい,敏速な / (時計が)『進んでいる』 / 『しっかりした』,ぐらつかない / 心の変わらない,忠実な(faithful) / (色が)あせない / 《古》(女性が)身持ちの悪い;《まれに》(生活が)享楽的な / (フイルムが)高感度の,(レンズが)高速撮影用の / 『速く』;すばやく(quickly) / 『しっかりと』,固く(firmly) / 『ぐっすりと』(soundly) / どしどし,ひっきりなしに
断食(だんじき),絶食 / (宗教的行事としての)断食日,断食期間 / 断食する,絶食する / (…で)精進する《+『on』+『名』》
『青い』,あい色の / 青黒い / 《話》陰気な,憂うつな / 〈U〉『青色』,あい色;青色の着物 / 〈U〉〈C〉青色絵の具,あい色染料 / 《the~》《詩》青空,青い海 / 《the blues》《話》気のふさぎ,うれいの色 / 《the blues》《ときに単数扱い》(ジャズ音楽の)ブルース / …'を'青色にする
(…で)…‘を'『汚す』,‘に'しみをつける《+『名』+『with』+『名』》 / 〈ガラス・木材・布など〉‘に'『着色する』 / (…で)〈人格・名声など〉‘を'汚す,‘に'傷をつける《+『名』+『with』+『名』》 / 汚れる,しみがつく / 〈C〉〈U〉(…についた)『しみ』,汚れ《+『on』+『名』》 / 〈U〉〈C〉着色剤,染料 / 〈C〉(人格・名声などに対する)汚点,傷《+『on』(『upon』)+『名』》

Wikipedia preview

出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2016/08/01 08:09:19」(JST)

wiki en

[Wiki en表示]

Micrograph of the pons using a hematoxylin & eosin-luxol fast blue stain.

Coronal section of a mouse brain stained with Hematoxylin & LFB

Luxol fast blue stain, abbreviated LFB stain or simply LFB, is a commonly used stain to observe myelin under light microscopy, created by Heinrich Klüver and Barrera in 1953.^[1] LFB is commonly used to detect demyelination in the central nervous system (CNS), but cannot discern myelination in the peripheral nervous system.^[2]

Procedure

Luxol fast blue is a copper phthalocyanine dye that is soluble in alcohol and is attracted to bases found in the lipoproteins of the myelin sheath.^[3]^[4]

Under the stain, myelin fibers appear blue, neuropil appears pink, and nerve cells appear purple. Tissues sections are treated over an extended period of time (usually overnight) and then differentiated with a lithium carbonate solution.^[4]

Combination methods

The combination of LFB with a variety of common staining methods provides the most useful and reliable method for the demonstration of pathological processes in the CNS.^[2] It is often combined with hematoxylin and eosin, which is abbreviated H-E-LFB, H&E-LFB. Other common staining methods include the periodic acid-Schiff, Oil Red O, phosphotungstic acid, and Holmes silver nitrate method.^[2]

Disease states

Studies have demonstrated that in both, human and mice, traumatic brain injury is associated with ongoing white matter degeneration with survival > 1 year post-injury.^[5]^[6]

References

^ Kluver, H., and Barrera, E. A method for the combined staining of cells and fibers in the Nervous system. J. Neuropath. Exp. Neurol. 12:400-403, 1953.
^ ^a ^b ^c Shin J. Oh (2002). Color Atlas of Nerve Biopsy Pathology. CRC Press. pp. 191–. ISBN 978-0-8493-1676-0. Retrieved 1 January 2013.
^ Bruce-Gregorios, Jocelyn. Histophatologic Techniques. Goodwill Trading Co., Inc. pp. 241–. ISBN 978-971-12-0270-5. Retrieved 1 January 2013.
^ ^a ^b Peter A. Humphrey; Louis P. Dehner; John D. Pfeifer (2008). The Washington Manual of Surgical Pathology: Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri. Lippincott Williams & Wilkins. pp. 681–. ISBN 978-0-7817-6527-5. Retrieved 1 January 2013.
^ Jonhson, VE; Stewart, JE (January 2013). "Inflammation and white matter degeneration persist for years after a single traumatic brain injury.". Brain. 136 (1): 28–42. doi:10.1093/brain/aws322. PMID 23365092.
^ Mouzon, B; Bachmeier, C (February 2014). "Chronic neuropathological and neurobehavioral changes in a repetitive mild traumatic brain injury model.". Ann. Neurol. 75 (2): 241–254. doi:10.1002/ana.24064. PMID 24243523.

Sheehan D, Hrapchak B, Theory and practice of Histotechnology, 2nd ed,1980, pp. 262–264, Battelle Press, Ohio.

UpToDate Contents

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MALDI imaging mass spectrometry analysis-A new approach for protein mapping in multiple sclerosis brain lesions.

Maccarrone G1, Nischwitz S2, Deininger SO3, Hornung J2, König FB4, Stadelmann C5, Turck CW6, Weber F7.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.J Chromatogr B Analyt Technol Biomed Life Sci.2017 Mar 15;1047:131-140. doi: 10.1016/j.jchromb.2016.07.001. Epub 2016 Jul 1.
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Japanese Journal

Comparison of Preparation Methods for the Evaluation of Nerve Axons

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ヒト延髄内側毛帯の神経線維構成

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「ルクソール・ファスト・ブルー染色」

　　[★]

英: luxol fast blue stain
同: ルクソール・ファスト青染色、LFB染色
関: ルクソール・ファストブルー液、染色法

ミエリンの染色に用いられる
ミエリンの塩基性のリポプロテインと塩基性の染色剤との置換により染色される。
ミエリンは青、好中球はピンク、神経細胞は紫に染まる

http://upload.wikimedia.org/wikipedia/commons/thumb/1/17/Pons_-_very_high_mag.jpg/800px-Pons_-_very_high_mag.jpg

参考

wikipedia en

http://en.wikipedia.org/wiki/Luxol_fast_blue_stain

「luxol fast blue stain solution」

　　[★] ルクソール・ファストブルー液

「fast」

　　[★]

adj.

1

早い、急速な(opp. slow)
すばやい、敏捷な。手の早い
短期間の
時間のかからない、手間の要らない
(時間が)早い、進んでいる。(はかりが)実際より大きな値を示す。夏時間の
高速用の、敏速な動きに適した

2

固着した、しっかりした、ぐらつかない(opp. loose)
固く締まった。しっかりした(縫い目・握り方など)
心の変わらぬ(constant)、忠実な
(色が)あせない、色あせしない、堅牢な。耐…性の。(菌)(破壊または染色に対して)耐性のある

acid-fast bacteria 抗酸菌

関: fasted、fasting、food deprivation、high speed、high-performance、high-speed、highperformance、quick、rapid、swift

「stain」

　　[★]

n.

染色

v.

染色する

関: coloring agent、dye、staining、tissue stain

「blue」

　　[★]

n.

青色、青

adj.

青い

関: cyan

「fasted」

　　[★]

adj.

絶食させた、絶食時の

関: fast、fasting、food deprivation

「bluing」

　　[★]

n.

ブルーイング、青味付け。反射防止

[1] Kluver, H., and Barrera, E. A method for the combined staining of cells and fibers in the Nervous system. J. Neuropath. Exp. Neurol. 12:400-403, 1953.

[Oh2002-2] Shin J. Oh (2002). Color Atlas of Nerve Biopsy Pathology. CRC Press. pp. 191–. ISBN 978-0-8493-1676-0. Retrieved 1 January 2013.

[Jocelyn-3] Bruce-Gregorios, Jocelyn. Histophatologic Techniques. Goodwill Trading Co., Inc. pp. 241–. ISBN 978-971-12-0270-5. Retrieved 1 January 2013.

[HumphreyDehner2008-4] Peter A. Humphrey; Louis P. Dehner; John D. Pfeifer (2008). The Washington Manual of Surgical Pathology: Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri. Lippincott Williams & Wilkins. pp. 681–. ISBN 978-0-7817-6527-5. Retrieved 1 January 2013.

[Jonhson_2013-5] Jonhson, VE; Stewart, JE (January 2013). "Inflammation and white matter degeneration persist for years after a single traumatic brain injury.". Brain. 136 (1): 28–42. doi:10.1093/brain/aws322. PMID 23365092.

[Mouzon2014-6] Mouzon, B; Bachmeier, C (February 2014). "Chronic neuropathological and neurobehavioral changes in a repetitive mild traumatic brain injury model.". Ann. Neurol. 75 (2): 241–254. doi:10.1002/ana.24064. PMID 24243523.

リンク元	「ルクソール・ファスト・ブルー染色」
拡張検索	「luxol fast blue stain solution」
関連記事	「fast」「stain」「blue」「fasted」「bluing」

匿名

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案内

案内

luxol fast blue stain