WordNet

with respect to private feelings; "inwardly, she was raging" (同)inside
relating to or existing in the mind or thoughts; "a concern with inward reflections"
toward the center or interior; "move the needle further inwards!" (同)inwards

PrepTutorEJDIC

全文を閲覧するには購読必要です。 To read the full text you will need to subscribe.

Electrophysiological patch clamp assay to monitor the action of opioid receptors.

Spahn V1, Nockemann D, Machelska H.
Methods in molecular biology (Clifton, N.J.).Methods Mol Biol.2015;1230:197-211. doi: 10.1007/978-1-4939-1708-2_16.
The patch clamp is a valuable electrophysiological technique, which allows the study of single or multiple ion channels in cells, and it is particularly useful in testing the excitable cells such as neurons. Activation of neuronal opioid receptors results in the modulation of various ion channels, w
PMID 25293327

Analysis of potassium and calcium imaging to assay the function of opioid receptors.

Spahn V1, Nockemann D, Machelska H.
Methods in molecular biology (Clifton, N.J.).Methods Mol Biol.2015;1230:187-96. doi: 10.1007/978-1-4939-1708-2_15.
As the activation of opioid receptors leads to the modulation of potassium and calcium channels, the ion imaging represents an attractive method to analyze the function of the receptors. Here, we describe the imaging of potassium using the FluxOR™ potassium ion channel assay, and of calcium using
PMID 25293326

Fluorescence-Based, High-Throughput Assays for μ-Opioid Receptor Activation Using a Membrane Potential-Sensitive Dye.

Knapman A1, Connor M.
Methods in molecular biology (Clifton, N.J.).Methods Mol Biol.2015;1230:177-85. doi: 10.1007/978-1-4939-1708-2_14.
The development of new and improved opioid analgesics requires high-throughput screening (HTS) methods to identify potential therapeutics from large libraries of lead compounds. Here we describe two simple, real-time fluorescence-based assays of μ-opioid receptor activation that may be scaled up fo
PMID 25293325

Epithelial Sheet Folding Induces Lumen Formation by Madin-Darby Canine Kidney Cells in a Collagen Gel

Ishida Sumire,Tanaka Ryosuke,Yamaguchi Naoya,Ogata Genki,Mizutani Takeomi,Kawabata Kazushige,Haga Hisashi
PLOS ONE 9(8), e99655, 2014-08-29
… Immediately after the collagen gel overlay, an epithelial sheet folded from the periphery, migrated inwardly, and formed a luminal structure. …
NAID 120005476150

Association Between KCNJ6 (GIRK2) Gene Polymorphism rs2835859 and Post-operative Analgesia, Pain Sensitivity, and Nicotine Dependence

Nishizawa Daisuke,Fukuda Ken-ichi,Kasai Shinya,Ogai Yasukazu,Hasegawa Junko,Sato Naomi,Yamada Hidetaka,Tanioka Fumihiko,Sugimura Haruhiko,Hayashida Masakazu,Ikeda Kazutaka
Journal of Pharmacological Sciences, 2014
… G-protein–activated inwardly rectifying potassium (GIRK) channels are expressed in many tissues and activated by several Gi/o protein–coupled receptors, such as opioid and dopamine receptors, and thus are known to be involved in the modulation of opioid-induced analgesia, pain, and reward. …
NAID 130004700283

村上慎吾,倉智嘉久
生体医工学 52(Supplement), O-414-O-415, 2014
… Astrocyte models were constructed by incorporating various mechanisms such as intra/extracellular ion concentrations of Na +, K +, and Cl -, cell volume, and models of Na,K-ATPase, Na-K-Cl cotransporter (NKCC), K-Cl cotransporter, inwardly-rectifying K+ …
NAID 130004695851