関: humanized mouse

WordNet

the syllable naming the third (mediant) note of any major scale in solmization
make more humane; "The mayor tried to humanize life in the big city" (同)humanise

PrepTutorEJDIC

ミ(全音階の第3音)
mouseの複数形

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1. バルトネラ属の基礎生物学 basic biology of bartonella species
2. Lobomycosis
3. 遺伝学およびゲノミクスのためのツール：研究用に育種された遺伝子改変マウス tools for genetics and genomics specially bred and genetically engineered mice
4. 周産期心筋症：治療および予後 peripartum cardiomyopathy treatment and prognosis
5. 肥満の病因 pathogenesis of obesity

English Journal

Pharmacological efficacy of anti-IL-1β scFv, Fab and full-length antibodies in treatment of rheumatoid arthritis.

Qi J, Ye X, Ren G, Kan F, Zhang Y, Guo M, Zhang Z, Li D.Author information Northeast Agricultural University, School of Life Science, No. 59 Mucai Street, Xiangfang District, 150030 Harbin, Heilongjiang, China.AbstractRheumatoid arthritis (RA) is a chronic autoimmune inflammatory disease that mainly causes the synovial joint inflammation and cartilage destruction. Interleukin-1β (IL-1β) is an important proinflammatory cytokine involved in the pathogenesis of RA. In this study, we constructed and expressed anti-IL-1β-full-length antibody in CHO-K1-SV, anti-IL-1β-Fab and anti-IL-1β-scFv in Rosetta. We compared the therapeutic efficacy of three anti-IL-1β antibodies for CIA mice. Mice with CIA were subcutaneously injected with humanized anti-IL-1β-scFv, anti-IL-1β-Fab or anti-IL-1β-full-length antibody. The effects of treatment were determined by arthritis severity score, autoreactive humoral, cellular immune responses, histological lesion and cytokines production. Compared with anti-IL-1β-scFv treatments, anti-IL-1β-Fab and anti-IL-1β-full-length antibody therapy resulted in more significant effect in alleviating the severity of arthritis by preventing bone damage and cartilage destruction, reducing humoral and cellular immune responses, and down-regulating the expression of IL-1β, IL-6, IL-2, IFN-γ, TNF-α and MMP-3 in inflammatory tissue. The therapeutic effects of anti-IL-1β-Fab and anti-IL-1β-full-length antibodies on CIA mice had no significant difference. However, production of anti-IL-1β-full-length antibody in eukaryotic system is, in general, time-consuming and more expensive than that of anti-IL-1β-Fab in prokaryotic systems. In conclusion, as a small molecule antibody, anti-IL-1β-Fab is an ideal candidate for RA therapy.
Molecular immunology.Mol Immunol.2014 Feb;57(2):59-65. doi: 10.1016/j.molimm.2013.08.002. Epub 2013 Sep 30.
Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disease that mainly causes the synovial joint inflammation and cartilage destruction. Interleukin-1β (IL-1β) is an important proinflammatory cytokine involved in the pathogenesis of RA. In this study, we constructed and expressed anti-
PMID 24091292

Alterations of brain anatomy in mouse model of MDD created by replacement of homologous mouse DNA sequence with an illness-associated 6-base human CREB1 promoter sequence.

Zubenko GS, Hughes HB, Hitchens TK, Cohen BM.Author information Department of Psychiatry, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania; Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania.AbstractWe have recently reported the creation and initial characterization of an etiology-based recombinant mouse model of a severe and inherited form of Major Depressive Disorder (MDD). This was achieved by replacing the corresponding mouse DNA sequence with a 6-base DNA sequence from the human CREB1 promoter that is associated with the development of MDD in men and women from families identified by probands with recurrent, early-onset MDD (RE-MDD). Individuals in these families are also at increased risk for childhood developmental disorders and late life neurodegenerative disorders. The current study used three-dimensional magnetic resonance microscopy (3D-MRM) to determine the effect of the resulting humanized mutation of the mouse Creb1 gene on the anatomy of the mouse brain. Homozygous mutant mice manifested prominent increases in the volume and surface area of the lateral ventricles, as well as reduced volume of the anterior corpus callosum, compared to age/sex-matched wild-type mice. No significant genotype effects were observed on the volume or surface area of total brain, or several brain regions sometimes observed to be abnormal in human depression, including hippocampus, amygdala, or striatum. These findings suggest that at least some forms of MDD result from abnormal brain development produced by inherited genetic variants. © 2013 Wiley Periodicals, Inc.
American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics.Am J Med Genet B Neuropsychiatr Genet.2014 Jan;165(1):1-8. doi: 10.1002/ajmg.b.32198. Epub 2013 Sep 4.
We have recently reported the creation and initial characterization of an etiology-based recombinant mouse model of a severe and inherited form of Major Depressive Disorder (MDD). This was achieved by replacing the corresponding mouse DNA sequence with a 6-base DNA sequence from the human CREB1 prom
PMID 24006268

Anti-CEA antibody fragments labeled with [18F]AlF for PET imaging of CEA-expressing tumors.

Lütje S, Franssen GM, Sharkey RM, Laverman P, Rossi EA, Goldenberg DM, Oyen WJ, Boerman OC, McBride W.AbstractA facile and rapid method to label peptides with 18F based on chelation of [18F]AlF has been developed recently. Since this method requires heating to 100 ºC, it cannot be used to label heat-sensitive proteins. Here, we used a two-step procedure to prepare 18F-labeled heat-labile proteins using the [18F]AlF method based on hot maleimide conjugation. 1,4,7-triazacyclononae-1,4-diacetate (NODA) containing a methyl phenylacetic acid group (MPA) functionalized with N-(2-aminoethyl)maleimide (EM) was used as a ligand which was labeled with [18F]AlF and then conjugated to the humanized anti-CEA antibody derivatives hMN-14 Fab', hMN-14-(scFv)2 (diabody) and a Dock-and-Lock engineered dimeric fragment hMN-14 Fab-AD2 at room temperature. The in vivo tumor targeting characteristics of the 18F-labeled antibody derivatives were determined by PET imaging of mice with s.c. xenografts. NODA-MPAEM was radiolabeled with [18F]AlF at a specific activity of 29-39 MBq/nmol and a labeling efficiency of 94 ± 2 %. The labeling efficiencies of the maleimide conjugation ranged from 70% to 77% resulting in [18F]AlF-labeled hMN14-Fab', hMN14-Fab-AD2 or hMN14-diabody with a specific activity of 15-17 MBq/nmol. The radiolabeled conjugates were purified by gel filtration. For biodistribution and microPET imaging, antibody fragments were injected intravenously into BALB/c nude mice with s.c. CEA-expressing LS174T xenografts (right flank) and CEA-negative SK-RC-52 xenografts (left flank). All [18F]AlF-labeled conjugates showed specific uptake in the LS174T xenografts with a maximal tumor uptake of 4.73 %ID/g at 1 h after injection. Uptake in CEA-negative SK-RC-52 xenografts was significantly lower. Tumors were clearly visualized on microPET images. Using a [18F]AlF-labeled maleimide functionalized chelator, antibody fragments could be radiofluorinated within one hour at high specific activity. Here, we translated this method to preclinical PET imaging studies and showed feasibility of [18F]AlF-fluorinated hMN-14-Fab', [18F]AlF-hMN-14-Fab-AD2, and [18F]AlF-hMN-14-diabody for microPET imaging of CEA-expressing colonic cancer.
Bioconjugate chemistry.Bioconjug Chem.2014 Jan 1. [Epub ahead of print]
A facile and rapid method to label peptides with 18F based on chelation of [18F]AlF has been developed recently. Since this method requires heating to 100 ºC, it cannot be used to label heat-sensitive proteins. Here, we used a two-step procedure to prepare 18F-labeled heat-labile proteins using the
PMID 24382090

Japanese Journal

Projections of nucleus accumbens adenosine A2A receptor neurons in the mouse brain and their implications in mediating sleep-wake regulation

Zhang Jian-Ping,Xu Qi,Yuan Xiang-Shan,Cherasse Yoan,Schiffmann Serge N.,de Kerchove d'Exaerde Alban,Qu Wei-Min,Urade Yoshihiro,Lazarus Michael,Huang Zhi-Li,Li Rui-Xi,ラザルスミハエル,裏出良博
Frontiers in Neuroanatomy 7, 43, 2013-12
… To better understand the functions of these receptors in sleep, projections of A(2A)R neurons were mapped utilizing adeno-associated virus (AAV) encoding humanized Renilla green fluorescent protein (hrGFP) as a tracer for long axonal pathways. … The Cre-dependent AAV was injected into the core (AcbC) and shell (AcbSh) of the Acb in A(2A)R-Cre mice. …
NAID 120005367979

Thromboxane A2 synthase inhibitors prevent production of infectious hepatitis C virus in mice with humanized livers.

Abe Yuichi,Aly Hussein Hassan,Hiraga Nobuhiko,Imamura Michio,Wakita Takaji,Shimotohno Kunitada,Chayama Kazuaki,Hijikata Makoto
Gastroenterology 145(3), 658-667.e11, 2013-09
… We investigated the effects of anti-HCV reagents that altered these signaling pathways in mice with humanized livers (carrying human hepatocytes). … The TXAS inhibitor and a prostaglandin I2 receptor agonist, which has effects that are opposite those of thromboxane A2, reduced serum levels of HCV and inhibited the infection of human hepatocytes by blood-borne HCV in mice. …
NAID 120005324909