human genome

ヒトゲノム

WordNet

1. the ordering of genes in a haploid set of chromosomes of a particular organism; the full DNA sequence of an organism; "the human genome contains approximately three billion chemical base pairs"
2. sing with closed lips; "She hummed a melody"
3. a humming noise; "the hum of distant traffic" (同)humming
4. be noisy with activity; "This office is buzzing with activity" (同)buzz, seethe
5. sound with a monotonous hum (同)thrum
6. make a low continuous sound; "The refrigerator is humming"
7. relating to a person; "the experiment was conducted on 6 monkeys and 2 human subjects"
8. characteristic of humanity; "human nature"
9. having human form or attributes as opposed to those of animals or divine beings; "human beings"; "the human body"; "human kindness"; "human frailty"

PrepTutorEJDIC

1. 〈ハチ・機械などが〉『ブンブンいう』,ブーンと鳴る / 『鼻歌を歌う』,ハミングスル / (ちゅうちょ・当惑で)ふむふむいう / 《話》〈雰囲気・事業などが〉活気がある,活発に動く;(…で)活気づく《+『with』+『名』》 / …‘を'『ハミングで歌う』,鼻歌を歌って…‘を'(ある状熊に)する / (ハチ・機械などの)『ブンブン』[『うなる音』];鼻歌 / (雑踏などの)ガヤガヤ]いう音],騒音 / ふ‐む,う‐ん(ちゅうちょ・当惑・黙考・疑惑・不快などの声)
2. (動物・神に対して)『人間の』,人の / 『人間らいし』,人間的な,人情味のある / 〈C〉《複数形》(動物に怠して)人間(human being) / 〈U〉《the human》人類

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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2016/07/04 02:53:15」(JST)

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For a non-technical introduction to the topic, see Introduction to genetics.

Genomic information

Graphical representation of the idealized human diploid karyotype, showing the organization of the genome into chromosomes. This drawing shows both the female (XX) and male (XY) versions of the 23rd chromosome pair. Chromosomes are shown aligned at their centromeres. The mitochondrial DNA is not shown.
NCBI genome ID	51
Ploidy	diploid
Genome size	3,234.83 Mb (Mega-basepairs)
Number of chromosomes	23 pairs

The human genome is the complete set of nucleic acid sequence for humans (Homo sapiens), encoded as DNA within the 23 chromosome pairs in cell nuclei and in a small DNA molecule found within individual mitochondria. Human genomes include both protein-coding DNA genes and noncoding DNA. Haploid human genomes, which are contained in germ cells (the egg and sperm gamete cells created in the meiosis phase of sexual reproduction before fertilization creates a zygote) consist of three billion DNA base pairs, while diploid genomes (found in somatic cells) have twice the DNA content. While there are significant differences among the genomes of human individuals (on the order of 0.1%), these are considerably smaller than the differences between humans and their closest living relatives, the chimpanzees (approximately 4%) and bonobos.

The Human Genome Project produced the first complete sequences of individual human genomes, with the first draft sequence and initial analysis being published on February 12, 2001. The human genome was the first of all vertebrates to be completely sequenced. As of 2012, thousands of human genomes have been completely sequenced, and many more have been mapped at lower levels of resolution. The resulting data are used worldwide in biomedical science, anthropology, forensics and other branches of science. There is a widely held expectation that genomic studies will lead to advances in the diagnosis and treatment of diseases, and to new insights in many fields of biology, including human evolution.

Although the sequence of the human genome has been (almost) completely determined by DNA sequencing, it is not yet fully understood. Most (though probably not all) genes have been identified by a combination of high throughput experimental and bioinformatics approaches, yet much work still needs to be done to further elucidate the biological functions of their protein and RNA products. Recent results suggest that most of the vast quantities of noncoding DNA within the genome have associated biochemical activities, including regulation of gene expression, organization of chromosome architecture, and signals controlling epigenetic inheritance.

There are an estimated 20,000-25,000 human protein-coding genes. The estimate of the number of human genes has been repeatedly revised down from initial predictions of 100,000 or more as genome sequence quality and gene finding methods have improved, and could continue to drop further. Protein-coding sequences account for only a very small fraction of the genome (approximately 1.5%), and the rest is associated with non-coding RNA molecules, regulatory DNA sequences, LINEs, SINEs, introns, and sequences for which as yet no function has been determined.

In June 2016, scientists formally announced HGP-Write, a plan to synthesize the human genome.

Molecular organization and gene content

See also: Lists of human genes by chromosome

The total length of the human genome is over 3 billion base pairs. The genome is organized into 22 paired chromosomes, plus the X chromosome (one in males, two in females) and, in males only, one Y chromosome. These are all large linear DNA molecules contained within the cell nucleus. The genome also includes the mitochondrial DNA, a comparatively small circular molecule present in each mitochondrion. Basic information about these molecules and their gene content, based on a reference genome that does not represent the sequence of any specific individual, are provided in the following table. (Data source: Ensembl genome browser release 68, July 2012)

Chromosome	Length (mm)	Base pairs	Variations	Confirmed proteins	Putative proteins	Pseudogenes	miRNA	rRNA	snRNA	snoRNA	Misc ncRNA	Links	Centromere position (Mbp)	Cumulative (%)
1	85	249,250,621	4,401,091	2,012	31	1,130	134	66	221	145	106	EBI	125.0	7.9
2	83	243,199,373	4,607,702	1,203	50	948	115	40	161	117	93	EBI	93.3	16.2
3	67	198,022,430	3,894,345	1,040	25	719	99	29	138	87	77	EBI	91.0	23.0
4	65	191,154,276	3,673,892	718	39	698	92	24	120	56	71	EBI	50.4	29.6
5	62	180,915,260	3,436,667	849	24	676	83	25	106	61	68	EBI	48.4	35.8
6	58	171,115,067	3,360,890	1,002	39	731	81	26	111	73	67	EBI	61.0	41.6
7	54	159,138,663	3,045,992	866	34	803	90	24	90	76	70	EBI	59.9	47.1
8	50	146,364,022	2,890,692	659	39	568	80	28	86	52	42	EBI	45.6	52.0
9	48	141,213,431	2,581,827	785	15	714	69	19	66	51	55	EBI	49.0	56.3
10	46	135,534,747	2,609,802	745	18	500	64	32	87	56	56	EBI	40.2	60.9
11	46	135,006,516	2,607,254	1,258	48	775	63	24	74	76	53	EBI	53.7	65.4
12	45	133,851,895	2,482,194	1,003	47	582	72	27	106	62	69	EBI	35.8	70.0
13	39	115,169,878	1,814,242	318	8	323	42	16	45	34	36	EBI	17.9	73.4
14	36	107,349,540	1,712,799	601	50	472	92	10	65	97	46	EBI	17.6	76.4
15	35	102,531,392	1,577,346	562	43	473	78	13	63	136	39	EBI	19.0	79.3
16	31	90,354,753	1,747,136	805	65	429	52	32	53	58	34	EBI	36.6	82.0
17	28	81,195,210	1,491,841	1,158	44	300	61	15	80	71	46	EBI	24.0	84.8
18	27	78,077,248	1,448,602	268	20	59	32	13	51	36	25	EBI	17.2	87.4
19	20	59,128,983	1,171,356	1,399	26	181	110	13	29	31	15	EBI	26.5	89.3
20	21	63,025,520	1,206,753	533	13	213	57	15	46	37	34	EBI	27.5	91.4
21	16	48,129,895	787,784	225	8	150	16	5	21	19	8	EBI	13.2	92.6
22	17	51,304,566	745,778	431	21	308	31	5	23	23	23	EBI	14.7	93.8
X	53	155,270,560	2,174,952	815	23	780	128	22	85	64	52	EBI	60.6	99.1
Y	20	59,373,566	286,812	45	8	327	15	7	17	3	2	EBI	12.5	100.0
mtDNA	0.0054	16,569	929	13	0	0	0	2	0	0	22	EBI	N/A	100.0
total		3,095,693,981		19,313	738	12,859

Table 1 (above) summarizes the physical organization and gene content of the human reference genome, with links to the original analysis, as published in the Ensembl database at the European Bioinformatics Institute (EBI) and Wellcome Trust Sanger Institute. Chromosome lengths were estimated by multiplying the number of base pairs by 0.34 nanometers, the distance between base pairs in the DNA double helix. The number of proteins is based on the number of initial precursor mRNA transcripts, and does not include products of alternative pre-mRNA splicing, or modifications to protein structure that occur after translation.

The number of variations is a summary of unique DNA sequence changes that have been identified within the sequences analyzed by Ensembl as of July, 2012; that number is expected to increase as further personal genomes are sequenced and examined. In addition to the gene content shown in this table, a large number of non-expressed functional sequences have been identified throughout the human genome (see below). Links open windows to the reference chromosome sequence in the EBI genome browser. The table also describes prevalence of genes encoding structural RNAs in the genome.

MicroRNA, or miRNA, functions as a post-transcriptional regulator of gene expression. Ribosomal RNA, or rRNA, makes up the RNA portion of the ribosome and is critical in the synthesis of proteins. Small nuclear RNA, or snRNA, is found in the nucleus of the cell. Its primary function is in the processing of pre-mRNA molecules and also in the regulation of transcription factors. Small nucleolar RNA, or SnoRNA, primarily functions in guiding chemical modifications to other RNA molecules.

Completeness of the human genome sequence

Although the human genome has been completely sequenced for all practical purposes, there are still hundreds of gaps in the sequence. A recent study noted more than 160 euchromatic gaps of which 50 gaps were closed. However, there are still numerous gaps in the heterochromatic parts of the genome which is much harder to sequence due to numerous repeats and other intractable sequence features.

Coding vs. noncoding DNA

The content of the human genome is commonly divided into coding and noncoding DNA sequences. Coding DNA is defined as those sequences that can be transcribed into mRNA and translated into proteins during the human life cycle; these sequences occupy only a small fraction of the genome (<2%). Noncoding DNA is made up of all of those sequences (ca. 98% of the genome) that are not used to encode proteins.

Some noncoding DNA contains genes for RNA molecules with important biological functions (noncoding RNA, for example ribosomal RNA and transfer RNA). The exploration of the function and evolutionary origin of noncoding DNA is an important goal of contemporary genome research, including the ENCODE (Encyclopedia of DNA Elements) project, which aims to survey the entire human genome, using a variety of experimental tools whose results are indicative of molecular activity.

Because non-coding DNA greatly outnumbers coding DNA, the concept of the sequenced genome has become a more focused analytical concept than the classical concept of the DNA-coding gene.

Mutation Rate of Human Genome

Mutation rate of human genome is a very important factor in calculating evolutionary time points. Researchers calculated the number of genetic variations between human and apes. Dividing that number by age of fossil of most recent common ancestor of humans and ape, researchers calculated the mutation rate. Recent studies using next generation sequencing technologies concluded a slow mutation rate which doesn't add up with human migration pattern time points and suggesting a new evolutionary time scale. 100,000 year old human fossils found in Israel have served to compound this new found uncertainty of the human migration timeline.

Coding sequences (protein-coding genes)

Human genes categorized by function of the transcribed proteins, given both as number of encoding genes and percentage of all genes.

Protein-coding sequences represent the most widely studied and best understood component of the human genome. These sequences ultimately lead to the production of all human proteins, although several biological processes (e.g. DNA rearrangements and alternative pre-mRNA splicing) can lead to the production of many more unique proteins than the number of protein-coding genes.

The complete modular protein-coding capacity of the genome is contained within the exome, and consists of DNA sequences encoded by exons that can be translated into proteins. Because of its biological importance, and the fact that it constitutes less than 2% of the genome, sequencing of the exome was the first major milepost of the Human Genome Project.

Number of protein-coding genes. About 20,000 human proteins have been annotated in databases such as Uniprot. Historically, estimates for the number of protein genes have varied widely, ranging up to 2,000,000 in the late 1960s, but several researchers pointed out in the early 1970s that the estimated mutational load from deleterious mutations placed an upper limit of approximately 40,000 for the total number of functional loci (this includes protein-coding and functional non-coding genes).

The number of human protein-coding genes is not significantly larger than that of many less complex organisms, such as the roundworm and the fruit fly. This difference may result from the extensive use of alternative pre-mRNA splicing in humans, which provides the ability to build a very large number of modular proteins through the selective incorporation of exons.

Protein-coding capacity per chromosome. Protein-coding genes are distributed unevenly across the chromosomes, ranging from a few dozen to more than 2000, with an especially high gene density within chromosomes 19, 11, and 1 (Table 1). Each chromosome contains various gene-rich and gene-poor regions, which may be correlated with chromosome bands and GC-content. The significance of these nonrandom patterns of gene density is not well understood.

Size of protein-coding genes. The size of protein-coding genes within the human genome shows enormous variability (Table 2). For example, the gene for histone H1a (HIST1HIA) is relatively small and simple, lacking introns and encoding mRNA sequences of 781 nt and a 215 amino acid protein (648 nt open reading frame). Dystrophin (DMD) is the largest protein-coding gene in the human reference genome, spanning a total of 2.2 MB, while Titin (TTN) has the longest coding sequence (114,414 bp), the largest number of exons (363), and the longest single exon (17,106 bp). Over the whole genome, the median size of an exon is 122 bp (mean = 145 bp), the median number of exons is 7 (mean = 8.8), and the median coding sequence encodes 367 amino acids (mean = 447 amino acids; Table 21 in ).

Protein	Chrom	Gene	Length	Exons	Exon length	Intron length	Alt splicing
Breast cancer type 2 susceptibility protein	13	BRCA2	83,736	27	11,386	72,350	yes
Cystic fibrosis transmembrane conductance regulator	7	CFTR	202,881	27	4,440	198,441	yes
Cytochrome b	MT	MTCYB	1,140	1	1,140	0	no
Dystrophin	X	DMD	2,220,381	79	10,500	2,209,881	yes
Glyceraldehyde-3-phosphate dehydrogenase	12	GAPDH	4,444	9	1,425	3,019	yes
Hemoglobin beta subunit	11	HBB	1,605	3	626	979	no
Histone H1A	6	HIST1H1A	781	1	781	0	no
Titin	2	TTN	281,434	364	104,301	177,133	yes

Table 2. Examples of human protein-coding genes. Chrom, chromosome. Alt splicing, alternative pre-mRNA splicing. (Data source: Ensembl genome browser release 68, July 2012)

Noncoding DNA (ncDNA)

Main article: Noncoding DNA

Noncoding DNA is defined as all of the DNA sequences within a genome that are not found within protein-coding exons, and so are never represented within the amino acid sequence of expressed proteins. By this definition, more than 98% of the human genomes is composed of ncDNA.

Numerous classes of noncoding DNA have been identified, including genes for noncoding RNA (e.g. tRNA and rRNA), pseudogenes, introns, untranslated regions of mRNA, regulatory DNA sequences, repetitive DNA sequences, and sequences related to mobile genetic elements.

Numerous sequences that are included within genes are also defined as noncoding DNA. These include genes for noncoding RNA (e.g. tRNA, rRNA), and untranslated components of protein-coding genes (e.g. introns, and 5' and 3' untranslated regions of mRNA).

Protein-coding sequences (specifically, coding exons) constitute less than 1.5% of the human genome. In addition, about 26% of the human genome is introns. Aside from genes (exons and introns) and known regulatory sequences (8–20%), the human genome contains regions of noncoding DNA. The exact amount of noncoding DNA that plays a role in cell physiology has been hotly debated. Recent analysis by the ENCODE project indicates that 80% of the entire human genome is either transcribed, binds to regulatory proteins, or is associated with some other biochemical activity.

It however remains controversial whether all of this biochemical activity contributes to cell physiology, or whether a substantial portion of this is the result transcriptional and biochemical noise, which must be actively filtered out by the organism. Excluding protein-coding sequences, introns, and regulatory regions, much of the non-coding DNA is composed of: Many DNA sequences that do not play a role in gene expression have important biological functions. Comparative genomics studies indicate that about 5% of the genome contains sequences of noncoding DNA that are highly conserved, sometimes on time-scales representing hundreds of millions of years, implying that these noncoding regions are under strong evolutionary pressure and positive selection.

Many of these sequences regulate the structure of chromosomes by limiting the regions of heterochromatin formation and regulating structural features of the chromosomes, such as the telomeres and centromeres. Other noncoding regions serve as origins of DNA replication. Finally several regions are transcribed into functional noncoding RNA that regulate the expression of protein-coding genes (for example ), mRNA translation and stability (see miRNA), chromatin structure (including histone modifications, for example ), DNA methylation (for example ), DNA recombination (for example ), and cross-regulate other noncoding RNAs (for example ). It is also likely that many transcribed noncoding regions do not serve any role and that this transcription is the product of non-specific RNA Polymerase activity.

Pseudogenes

Main article: Pseudogene

Pseudogenes are inactive copies of protein-coding genes, often generated by gene duplication, that have become nonfunctional through the accumulation of inactivating mutations. Table 1 shows that the number of pseudogenes in the human genome is on the order of 13,000, and in some chromosomes is nearly the same as the number of functional protein-coding genes. Gene duplication is a major mechanism through which new genetic material is generated during molecular evolution.

For example, the olfactory receptor gene family is one of the best-documented examples of pseudogenes in the human genome. More than 60 percent of the genes in this family are non-functional pseudogenes in humans. By comparison, only 20 percent of genes in the mouse olfactory receptor gene family are pseudogenes. Research suggests that this is a species-specific characteristic, as the most closely related primates all have proportionally fewer pseudogenes. This genetic discovery helps to explain the less acute sense of smell in humans relative to other mammals.

Genes for noncoding RNA (ncRNA)

Main article: Noncoding RNA

Noncoding RNA molecules play many essential roles in cells, especially in the many reactions of protein synthesis and RNA processing. Noncoding RNA include tRNA, ribosomal RNA, microRNA, snRNA and other non-coding RNA genes including about 60,000 long non coding RNAs (lncRNAs). It should be noted that while the number of reported lncRNA genes continues to rise and the exact number in the human genome is yet to be defined, many of them are argued to be non-functional.

Many ncRNAs are critical elements in gene regulation and expression. Noncoding RNA also contributes to epigenetics, transcription, RNA splicing, and the translational machinery. The role of RNA in genetic regulation and disease offers a new potential level of unexplored genomic complexity.

Introns and untranslated regions of mRNA

In addition to the ncRNA molecules that are encoded by discrete genes, the initial transcripts of protein coding genes usually contain extensive noncoding sequences, in the form of introns, 5'-untranslated regions (5'-UTR), and 3'-untranslated regions (3'-UTR). Within most protein-coding genes of the human genome, the length of intron sequences is 10- to 100-times the length of exon sequences (Table 2).

Regulatory DNA sequences

The human genome has many different regulatory sequences which are crucial to controlling gene expression. Conservative estimates indicate that these sequences make up 8% of the genome, however extrapolations from the ENCODE project give that 20-40% of the genome is gene regulatory sequence. Some types of non-coding DNA are genetic "switches" that do not encode proteins, but do regulate when and where genes are expressed (called enhancers).

Regulatory sequences have been known since the late 1960s. The first identification of regulatory sequences in the human genome relied on recombinant DNA technology. Later with the advent of genomic sequencing, the identification of these sequences could be inferred by evolutionary conservation. The evolutionary branch between the primates and mouse, for example, occurred 70–90 million years ago. So computer comparisons of gene sequences that identify conserved non-coding sequences will be an indication of their importance in duties such as gene regulation.

Other genomes have been sequenced with the same intention of aiding conservation-guided methods, for exampled the pufferfish genome. However, regulatory sequences disappear and re-evolve during evolution at a high rate.

As of 2012, the efforts have shifted toward finding interactions between DNA and regulatory proteins by the technique ChIP-Seq, or gaps where the DNA is not packaged by histones (DNase hypersensitive sites), both of which tell where there are active regulatory sequences in the investigated cell type.

Repetitive DNA sequences

Repetitive DNA sequences comprise approximately 50% of the human genome.

About 8% of the human genome consists of tandem DNA arrays or tandem repeats, low complexity repeat sequences that have multiple adjacent copies (e.g. "CAGCAGCAG..."). The tandem sequences may be of variable lengths, from two nucleotides to tens of nucleotides. These sequences are highly variable, even among closely related individuals, and so are used for genealogical DNA testing and forensic DNA analysis.

Repeated sequences of fewer than ten nucleotides (e.g. the dinucleotide repeat (AC)_n) are termed microsatellite sequences. Among the microsatellite sequences, trinucleotide repeats are of particular importance, as sometimes occur within coding regions of genes for proteins and may lead to genetic disorders. For example, Huntington's disease results from an expansion of the trinucleotide repeat (CAG)_n within the Huntingtin gene on human chromosome 4. Telomeres (the ends of linear chromosomes) end with a microsatellite hexanucleotide repeat of the sequence (TTAGGG)_n.

Tandem repeats of longer sequences (arrays of repeated sequences 10–60 nucleotides long) are termed minisatellites.

Mobile genetic elements (transposons) and their relics

Transposable genetic elements, DNA sequences that can replicate and insert copies of themselves at other locations within a host genome, are an abundant component in the human genome. The most abundant transposon lineage, Alu, has about 50,000 active copies, and can be inserted into intragenic and intergenic regions. One other lineage, LINE-1, has about 100 active copies per genome (the number varies between people). Together with non-functional relics of old transposons, they account for over half of total human DNA. Sometimes called "jumping genes", transposons have played a major role in sculpting the human genome. Some of these sequences represent endogenous retroviruses, DNA copies of viral sequences that have become permanently integrated into the genome and are now passed on to succeeding generations.

Mobile elements within the human genome can be classified into LTR retrotransposons (8.3% of total genome), SINEs (13.1% of total genome) including Alu elements, LINEs (20.4% of total genome), SVAs and Class II DNA transposons (2.9% of total genome).

Genomic variation in humans

Main articles: Human genetic variation and Human genetic clustering

Human Reference Genome

With the exception of identical twins, all humans show significant variation in genomic DNA sequences. The Human Reference Genome (HRG) is used as a standard sequence reference.

There are several important points concerning the Human Reference Genome--

• The HRG is a haploid sequence. Each chromosome is represented once.
• The HRG is a composite sequence, and does not correspond to any actual human individual.
• The HRG is periodically updated to correct errors and ambiguities.
• The HRG in no way represents an "ideal" or "perfect" human individual. It is simply a standardized representation or model that is used for comparative purposes.

Measuring human genetic variation

Most studies of human genetic variation have focused on single-nucleotide polymorphisms (SNPs), which are substitutions in individual bases along a chromosome. Most analyses estimate that SNPs occur 1 in 1000 base pairs, on average, in the euchromatic human genome, although they do not occur at a uniform density. Thus follows the popular statement that "we are all, regardless of race, genetically 99.9% the same", although this would be somewhat qualified by most geneticists. For example, a much larger fraction of the genome is now thought to be involved in copy number variation. A large-scale collaborative effort to catalog SNP variations in the human genome is being undertaken by the International HapMap Project.

The genomic loci and length of certain types of small repetitive sequences are highly variable from person to person, which is the basis of DNA fingerprinting and DNA paternity testing technologies. The heterochromatic portions of the human genome, which total several hundred million base pairs, are also thought to be quite variable within the human population (they are so repetitive and so long that they cannot be accurately sequenced with current technology). These regions contain few genes, and it is unclear whether any significant phenotypic effect results from typical variation in repeats or heterochromatin.

Most gross genomic mutations in gamete germ cells probably result in inviable embryos; however, a number of human diseases are related to large-scale genomic abnormalities. Down syndrome, Turner Syndrome, and a number of other diseases result from nondisjunction of entire chromosomes. Cancer cells frequently have aneuploidy of chromosomes and chromosome arms, although a cause and effect relationship between aneuploidy and cancer has not been established.

Mapping human genomic variation

Whereas a genome sequence lists the order of every DNA base in a genome, a genome map identifies the landmarks. A genome map is less detailed than a genome sequence and aids in navigating around the genome.

An example of a variation map is the HapMap being developed by the International HapMap Project. The HapMap is a haplotype map of the human genome, "which will describe the common patterns of human DNA sequence variation." It catalogs the patterns of small-scale variations in the genome that involve single DNA letters, or bases.

Researchers published the first sequence-based map of large-scale structural variation across the human genome in the journal Nature in May 2008. Large-scale structural variations are differences in the genome among people that range from a few thousand to a few million DNA bases; some are gains or losses of stretches of genome sequence and others appear as re-arrangements of stretches of sequence. These variations include differences in the number of copies individuals have of a particular gene, deletions, translocations and inversions.

SNP Frequency across the Human Genome

Single-nucleotide polymorphisms (SNPs) do not occur homogeneously across the human genome. In fact, there is enormous diversity in SNP frequency between genes, reflecting different selective pressures on each gene as well as different mutation and recombination rates across the genome. However, studies on SNPs are biased towards coding regions, the data generated from them are unlikely to reflect the overall distribution of SNPs throughout the genome. Therefore, the SNP Consortium protocol was designed to identify SNPs with no bias towards coding regions and the Consortium's 100,000 SNPs generally reflect sequence diversity across the human chromosomes.The SNP Consortium aims to expand the number of SNPs identified across the genome to 300 000 by the end of the first quarter of 2001.

TSC SNP distribution along the long arm of chromosome 22 (from http://snp.cshl.org/ ). Each column represents a 1 Mb interval; the approximate cytogenetic position is given on the x-axis. Clear peaks and troughs of SNP density can be seen, possibly reflecting different rates of mutation, recombination and selection.

Changes in non-coding sequence and synonymous changes in coding sequence are generally more common than non-synonymous changes, reflecting greater selective pressure reducing diversity at positions dictating amino acid identity. Transitional changes are more common than transversions, with CpG dinucleotides showing the highest mutation rate, presumably due to deamination.

Personal genomes

See also: Personal genomics

A personal genome sequence is a (nearly) complete sequence of the chemical base pairs that make up the DNA of a single person. Because medical treatments have different effects on different people due to genetic variations such as single-nucleotide polymorphisms (SNPs), the analysis of personal genomes may lead to personalized medical treatment based on individual genotypes.

The first personal genome sequence to be determined was that of Craig Venter in 2007. Personal genomes had not been sequenced in the public Human Genome Project to protect the identity of volunteers who provided DNA samples. That sequence was derived from the DNA of several volunteers from a diverse population. However, early in the Venter-led Celera Genomics genome sequencing effort the decision was made to switch from sequencing a composite sample to using DNA from a single individual, later revealed to have been Venter himself. Thus the Celera human genome sequence released in 2000 was largely that of one man. Subsequent replacement of the early composite-derived data and determination of the diploid sequence, representing both sets of chromosomes, rather than a haploid sequence originally reported, allowed the release of the first personal genome. In April 2008, that of James Watson was also completed. Since then hundreds of personal genome sequences have been released, including those of Desmond Tutu, and of a Paleo-Eskimo. In November 2013, a Spanish family made their personal genomics data obtained by direct-to-consumer genetic testing with 23andMe publicly available under a Creative Commons public domain license. This is believed to be the first such public genomics dataset for a whole family.

The sequencing of individual genomes further unveiled levels of genetic complexity that had not been appreciated before. Personal genomics helped reveal the significant level of diversity in the human genome attributed not only to SNPs but structural variations as well. However, the application of such knowledge to the treatment of disease and in the medical field is only in its very beginnings. Exome sequencing has become increasingly popular as a tool to aid in diagnosis of genetic disease because the exome contributes only 1% of the genomic sequence but accounts for roughly 85% of mutations that contribute significantly to disease.

Human genetic disorders

For more details on this topic, see Genetic disorder.

Most aspects of human biology involve both genetic (inherited) and non-genetic (environmental) factors. Some inherited variation influences aspects of our biology that are not medical in nature (height, eye color, ability to taste or smell certain compounds, etc.). Moreover, some genetic disorders only cause disease in combination with the appropriate environmental factors (such as diet). With these caveats, genetic disorders may be described as clinically defined diseases caused by genomic DNA sequence variation. In the most straightforward cases, the disorder can be associated with variation in a single gene. For example, cystic fibrosis is caused by mutations in the CFTR gene, and is the most common recessive disorder in caucasian populations with over 1,300 different mutations known.

Disease-causing mutations in specific genes are usually severe in terms of gene function, and are fortunately rare, thus genetic disorders are similarly individually rare. However, since there are many genes that can vary to cause genetic disorders, in aggregate they constitute a significant component of known medical conditions, especially in pediatric medicine. Molecularly characterized genetic disorders are those for which the underlying causal gene has been identified, currently there are approximately 2,200 such disorders annotated in the OMIM database.

Studies of genetic disorders are often performed by means of family-based studies. In some instances population based approaches are employed, particularly in the case of so-called founder populations such as those in Finland, French-Canada, Utah, Sardinia, etc. Diagnosis and treatment of genetic disorders are usually performed by a geneticist-physician trained in clinical/medical genetics. The results of the Human Genome Project are likely to provide increased availability of genetic testing for gene-related disorders, and eventually improved treatment. Parents can be screened for hereditary conditions and counselled on the consequences, the probability it will be inherited, and how to avoid or ameliorate it in their offspring.

As noted above, there are many different kinds of DNA sequence variation, ranging from complete extra or missing chromosomes down to single nucleotide changes. It is generally presumed that much naturally occurring genetic variation in human populations is phenotypically neutral, i.e. has little or no detectable effect on the physiology of the individual (although there may be fractional differences in fitness defined over evolutionary time frames). Genetic disorders can be caused by any or all known types of sequence variation. To molecularly characterize a new genetic disorder, it is necessary to establish a causal link between a particular genomic sequence variant and the clinical disease under investigation. Such studies constitute the realm of human molecular genetics.

With the advent of the Human Genome and International HapMap Project, it has become feasible to explore subtle genetic influences on many common disease conditions such as diabetes, asthma, migraine, schizophrenia, etc. Although some causal links have been made between genomic sequence variants in particular genes and some of these diseases, often with much publicity in the general media, these are usually not considered to be genetic disorders per se as their causes are complex, involving many different genetic and environmental factors. Thus there may be disagreement in particular cases whether a specific medical condition should be termed a genetic disorder. The categorized table below provides the prevalence as well as the genes or chromosomes associated with some human genetic disorders.

Disorder	Prevalence	Chromosome or gene involved
Chromosomal conditions
Down syndrome	1:600	Chromosome 21
Klinefelter syndrome	1:500–1000 males	Additional X chromosome
Turner syndrome	1:2000 females	Loss of X chromosome
Sickle cell anemia	1 in 50 births in parts of Africa; rarer elsewhere	β-globin (on chromosome 11)
Cancers
Breast/Ovarian cancer (susceptibility)	~5% of cases of these cancer types	BRCA1, BRCA2
FAP (hereditary nonpolyposis coli)	1:3500	APC
Lynch syndrome	5–10% of all cases of bowel cancer	MLH1, MSH2, MSH6, PMS2
Neurological conditions
Huntington disease	1:20000	Huntingtin
Alzheimer disease ‐ early onset	1:2500	PS1, PS2, APP
Other conditions
Cystic fibrosis	1:2500	CFTR
Duchenne muscular dystrophy	1:3500 boys	Dystrophin

Evolution

See also: Human evolution and Chimpanzee Genome Project

Comparative genomics studies of mammalian genomes suggest that approximately 5% of the human genome has been conserved by evolution since the divergence of extant lineages approximately 200 million years ago, containing the vast majority of genes. The published chimpanzee genome differs from that of the human genome by 1.23% in direct sequence comparisons. Around 20% of this figure is accounted for by variation within each species, leaving only ~1.06% consistent sequence divergence between humans and chimps at shared genes. This nucleotide by nucleotide difference is dwarfed, however, by the portion of each genome that is not shared, including around 6% of functional genes that are unique to either humans or chimps.

In other words, the considerable observable differences between humans and chimps may be due as much or more to genome level variation in the number, function and expression of genes rather than DNA sequence changes in shared genes. Indeed, even within humans, there has been found to be a previously unappreciated amount of copy number variation (CNV) which can make up as much as 5 – 15% of the human genome. In other words, between humans, there could be +/- 500,000,000 base pairs of DNA, some being active genes, others inactivated, or active at different levels. The full significance of this finding remains to be seen. On average, a typical human protein-coding gene differs from its chimpanzee ortholog by only two amino acid substitutions; nearly one third of human genes have exactly the same protein translation as their chimpanzee orthologs. A major difference between the two genomes is human chromosome 2, which is equivalent to a fusion product of chimpanzee chromosomes 12 and 13. (later renamed to chromosomes 2A and 2B, respectively).

Humans have undergone an extraordinary loss of olfactory receptor genes during our recent evolution, which explains our relatively crude sense of smell compared to most other mammals. Evolutionary evidence suggests that the emergence of color vision in humans and several other primate species has diminished the need for the sense of smell.

Mitochondrial DNA

The human mitochondrial DNA is of tremendous interest to geneticists, since it undoubtedly plays a role in mitochondrial disease. It also sheds light on human evolution; for example, analysis of variation in the human mitochondrial genome has led to the postulation of a recent common ancestor for all humans on the maternal line of descent (see Mitochondrial Eve).

Due to the lack of a system for checking for copying errors, mitochondrial DNA (mtDNA) has a more rapid rate of variation than nuclear DNA. This 20-fold higher mutation rate allows mtDNA to be used for more accurate tracing of maternal ancestry. Studies of mtDNA in populations have allowed ancient migration paths to be traced, such as the migration of Native Americans from Siberia or Polynesians from southeastern Asia. It has also been used to show that there is no trace of Neanderthal DNA in the European gene mixture inherited through purely maternal lineage. Due to the restrictive all or none manner of mtDNA inheritance, this result (no trace of Neanderthal mtDNA) would be likely unless there were a large percentage of Neanderthal ancestry, or there was strong positive selection for that mtDNA (for example, going back 5 generations, only 1 of your 32 ancestors contributed to your mtDNA, so if one of these 32 was pure Neanderthal you would expect that ~3% of your autosomal DNA would be of Neanderthal origin, yet you would have a ~97% chance to have no trace of Neanderthal mtDNA).

Epigenome

See also: Epigenetics

Epigenetics describes a variety of features of the human genome that transcend its primary DNA sequence, such as chromatin packaging, histone modifications and DNA methylation, and which are important in regulating gene expression, genome replication and other cellular processes. Epigenetic markers strengthen and weaken transcription of certain genes but do not affect the actual sequence of DNA nucleotides. DNA methylation is a major form of epigenetic control over gene expression and one of the most highly studied topics in epigenetics. During development, the human DNA methylation profile experiences dramatic changes. In early germ line cells, the genome has very low methylation levels. These low levels generally describe active genes. As development progresses, parental imprinting tags lead to increased methylation activity.

Epigenetic patterns can be identified between tissues within an individual as well as between individuals themselves. Identical genes that have differences only in their epigenetic state are called epialleles. Epialleles can be placed into three categories: those directly determined by an individual’s genotype, those influenced by genotype, and those entirely independent of genotype. The epigenome is also influenced significantly by environmental factors. Diet, toxins, and hormones impact the epigenetic state. Studies in dietary manipulation have demonstrated that methyl-deficient diets are associated with hypomethylation of the epigenome. Such studies establish epigenetics as an important interface between the environment and the genome.

See also

• Genetics
• Genomics
• Genographic Project
• Genomic organization
• Low copy repeats
• Noncoding DNA
• Whole genome sequencing
• Universal Declaration on the Human Genome and Human Rights

References

External links

• The National Human Genome Research Institute
• Ensembl The Ensembl Genome Browser Project
• National Library of Medicine human genome viewer
• UCSC Genome Browser.
• Human Genome Project.
• The National Office of Public Health Genomics
• New findings challenge established views about human genome
• INMEGEN: Complete genetic map of some mexican native groups
• Missing bits of DNA may define humans

UpToDate Contents

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• 1. ゲノム疾患：概要 genomic disorders an overview
• 2. 遺伝学およびゲノミクスのためのツール：モデルシステム tools for genetics and genomics model systems
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• 5. 遺伝カウンセリングおよび遺伝子検査 genetic counseling and testing

English Journal

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• Alzheimer's disease (AD) is known to be a multifactorial neurodegenerative disorder, and is one of the main causes of dementia in the elderly. Many studies have demonstrated molecules involved in the pathogenesis of AD, however its underlying mechanisms remain obscure. It may be simplistic to try to
• PMID 26235086

• New Genome-Wide Methods for Elucidation of Candidate Copy Number Variations (CNVs) Contributing to Alzheimer's Disease Heritability.

• Szigeti K1.
• Methods in molecular biology (Clifton, N.J.).Methods Mol Biol.2016;1303:315-26. doi: 10.1007/978-1-4939-2627-5_19.
• The complexity of human genetic variation has been extended by the observation of abundant and widespread variation in the copy number of submicroscopic DNA segments. The discovery of this novel level of genome organization opened new possibilities concerning the genetic variation that may confer su
• PMID 26235076

• Pooled-DNA Sequencing for Elucidating New Genomic Risk Factors, Rare Variants Underlying Alzheimer's Disease.

• Jin SC1, Benitez BA, Deming Y, Cruchaga C.
• Methods in molecular biology (Clifton, N.J.).Methods Mol Biol.2016;1303:299-314. doi: 10.1007/978-1-4939-2627-5_18.
• Analyses of genome-wide association studies (GWAS) for complex disorders usually identify common variants with a relatively small effect size that only explain a small proportion of phenotypic heritability. Several studies have suggested that a significant fraction of heritability may be explained b
• PMID 26235075

Japanese Journal

• JB Review : Goldfish : an old and new model system to study vertebrate development, evolution and human disease

• Omori Yoshihiro,Kon Tetsuo
• The journal of biochemistry 165(3), 209-218, 2019-03
• NAID 40021845851

• がんゲノム医療における人工知能 : がんの理解は人知を超えてしまった (特集 人工知能の医療応用Update)

• 宮野 悟
• Brain and nerve 71(1), 25-32, 2019-01
• NAID 40021780919

• Ｔｈｅ　Ｋｅｉｏ　Ｊｏｕｒｎａｌ　ｏｆ　Ｍｅｄｉｃｉｎｅ

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• Ｔｈｅ　Ｋｅｉｏ　Ｊｏｕｒｎａｌ　ｏｆ　Ｍｅｄｉｃｉｎｅ 68(1), 26-26, 2019
• … We are exploring this natural diversity through bioinformatics, biochemical, and molecular work to better understand the fundamental ways in which microbes and other living organisms sense and respond to their environment and as possible to develop these natural systems as molecular tools and to improve human health. …
• NAID 130007620438

Related Links

• National Human Genome Research Institute (NHGRI) - Homepage

Home page of the National Human Genome Research Institute, the arm of the U.S. National Institutes of Health dedicated to advancing human health through genetic research. ... Video now available Genomic Medicine Meeting VIII ...

• All About The Human Genome Project (HGP) - National ...

Introduction to the Human Genome Project, published by the National Human Genome Research Institute. This brief overview is aimed at students, teachers and other non-scientists. ... All About The Human Genome Project (HGP) ...

Related Pictures

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★リンクテーブル★

リンク元	「ヒトゲノム」
拡張検索	「human genome sequence」「human genome draft sequence」
関連記事	「genome」「human」

「ヒトゲノム」

　　[★]

英

human genome

• ヒトゲノムのシークエンシング：解読作業は1991年から開始。2000年6月26日にドラフト配列の解読終了。2003年4月14日に解読完了。

「human genome sequence」

　　[★]

• ヒトゲノム配列、ヒトゲノム塩基配列

「human genome draft sequence」

　　[★]

• ヒトゲノムドラフト配列

「genome」

　　[★] <additional_journal_mednt> <status>20220</status> <pubmed_ins date=20110329>

• Production and characterization of Acidothermus cellulolyticus endoglucanase in Pichia pastoris.

• Lindenmuth BE, McDonald KA.
• Protein expression and purification.2011 Jun;77(2):153-8. Epub 2011 Jan 22.
• PMID 21262363

• Differential activities of the Drosophila JAK/STAT pathway ligands Upd, Upd2 and Upd3.

• Wright VM, Vogt KL, Smythe E, Zeidler MP.
• Cellular signalling.2011 May;23(5):920-7. Epub 2011 Jan 22.
• PMID 21262354

• Adaptive haemoglobin gene control in Daphnia pulex at different oxygen and temperature conditions.

• Gerke P, Börding C, Zeis B, Paul RJ.
• Comparative biochemistry and physiology. Part A, Molecular & integrative physiology.2011 May;159(1):56-65. Epub 2011 Jan 31.
• PMID 21281731

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Genome - Wikipedia, the free encyclopedia

In modern molecular biology and genetics, the genome is the entirety of an organism's hereditary information. It is encoded either in DNA or, for many types of virus, in RNA. The genome includes both the genes and the non-coding ...

[display]http://r.gnavi.co.jp/k506200

ぐるなび - 心斎橋 個室ダイニング Genome．

心斎橋 個室ダイニング Genome．(大阪府/地下鉄御堂筋線心斎橋駅、誕生日・大人の空間 )の店舗情報です。ぐるなびなら詳細なメニューの情報や地図など、「心斎橋 個室 ダイニング Genome．」の情報が満載です。【心斎橋】イタリアン部門 アクセス ランキング ...

[display]http://pubs.nrc-cnrc.gc.ca/cgi-bin/rp/rp2_desc_e?gen

Genome - NRC Research Press

3 Mar 2011 ... P. Donini (Philip Morris Products S.A., Switzerland), Cereal genetics, plant molecular genetics, plant molecular breeding, biodiversity, genome evolution, classical and molecular cytogenetics ...

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「human」

　　[★]

• n.

• ヒト

• adj.

• ヒトの、ヒト型の

関

ape、hominid、Hominidae、homo、Homo sapiens、human being、human race、human-type、man、Pongidae