同: HRP

WordNet

coarse Eurasian plant cultivated for its thick white pungent root (同)horse radish, red cole, Armoracia_rusticana
the root of the horseradish plant; it is grated or ground and used for seasoning (同)horseradish root
grated horseradish root
any of a group of enzymes (occurring especially in plant cells) that catalyze the oxidation of a compound by a peroxide

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西洋ワサビ

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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2015/01/21 19:34:55」(JST)

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The enzyme horseradish peroxidase (HRP), found in the roots of horseradish, is used extensively in biochemistry applications primarily for its ability to amplify a weak signal and increase detectability of a target molecule. It is a metalloenzyme with many isoforms, of which the most studied type is C.

Structure

The structure of the enzyme was first solved by X-ray crystallography in 1997^[2] and has since has been solved several times with various substrates.^[3] It is an all alpha-helical protein which binds heme as a cofactor.

Substrates

Alone, the HRP enzyme, or conjugates thereof, is of little value; its presence must be made visible using a substrate that, when oxidized by HRP using hydrogen peroxide as the oxidizing agent, yields a characteristic change that is detectable by spectrophotometric methods.^[4]^[5]

Numerous substrates for the horseradish peroxidase enzyme have been described and commercialized to exploit the desirable features of HRP. These substrates fall into several distinct categories. HRP catalyzes the conversion of chromogenic substrates (e.g., TMB, DAB, ABTS) into colored products, and produces light when acting on chemiluminescent substrates (e.g. ECL).

Applications

Horseradish peroxidase is a 44,173.9-dalton glycoprotein with 6 lysine residues which can be conjugated to a labeled molecule. It produces a coloured, fluorimetric, or luminescent derivative of the labeled molecule when incubated with a proper substrate, allowing it to be detected and quantified. HRP is often used in conjugates (molecules that have been joined genetically or chemically) to determine the presence of a molecular target. For example, an antibody conjugated to HRP may be used to detect a small amount of a specific protein in a western blot. Here, the antibody provides the specificity to locate the protein of interest, and the HRP enzyme, in the presence of a substrate, produces a detectable signal.^[6] Horseradish peroxidase is also commonly used in techniques such as ELISA and Immunohistochemistry due to its monomeric nature and the ease with which it produces coloured products. Peroxidase, a heme-containing oxidoreductase, is a commercially important enzyme which catalyses the reductive cleavage of hydrogen peroxide by an electron donor.

Horseradish peroxidase is ideal in many respects for these applications because it is smaller, more stable, and less expensive than other popular alternatives such as alkaline phosphatase. It also has a high turnover rate that allows generation of strong signals in a relatively short time span.^{[citation needed]}

Moreover, "In recent years the technique of marking neurons with the enzyme horseradish peroxidase has become a major tool. In its brief history, this method has probably been used by more neurobiologists than have used the Golgi stain since its discovery in 1870."^[7]

Enhanced chemiluminescence (ECL)

Horseradish peroxidase catalyses the oxidation of luminol to 3-aminophthalate via several intermediates. The reaction is accompanied by emission of low-intensity light at 428 nm. However, in the presence of certain chemicals, the light emitted is enhanced up to 1000-fold, making the light easier to detect and increasing the sensitivity of the reaction. The enhancement of light emission is called enhanced chemiluminescence (ECL). Several enhancers can be used, but the most effective are modified phenols, especially p-iodophenol. The intensity of light is a measure of the number of enzyme molecules reacting and thus of the amount of hybrid. ECL is simple to set up and is sensitive, detecting about 0.5 pg nucleic acid in Southern blots and in northern blots. Detection by chemiluminescent substrates has several advantages over chromogenic substrates. The sensitivity is 10- to 100-fold greater, and quantifying of light emission is possible over a wide dynamic range, whereas that for coloured precipitates is much more limited, about one order of magnitude less. Stripping filters are much easier when chemiluminescent substrates are used.

References

^ PDB 1w4y; Carlsson GH, Nicholls P, Svistunenko D, Berglund GI, Hajdu J (January 2005). "Complexes of horseradish peroxidase with formate, acetate, and carbon monoxide". Biochemistry 44 (2): 635–42. doi:10.1021/bi0483211. PMID 15641789.
^ PDB 1GWU; Gajhede M, Schuller DJ, Henriksen A, Smith AT, Poulos TL (December 1997). "Crystal structure of horseradish peroxidase C at 2.15 A resolution". Nat. Struct. Biol. 4 (12): 1032–8. doi:10.1038/nsb1297-1032. PMID 9406554.
^ "Peroxidase C1A Related PDB sequences". UniPDB. European Bioinformatics Institute.
^ Veitch NC (February 2004). "Horseradish peroxidase: a modern view of a classic enzyme". Phytochemistry 65 (3): 249–59. doi:10.1016/j.phytochem.2003.10.022. PMID 14751298.
^ Akkara JA, Senecal KJ, Kaplan DL (October 1991). "Synthesis and characterization of polymers produced by horseradish peroxidase in dioxane". Journal of Polymer Science 29 (11): 1561–74. doi:10.1002/pola.1991.080291105.
^ Chau YP, Lu KS (1995). "Investigation of the blood-ganglion barrier properties in rat sympathetic ganglia by using lanthanum ion and horseradish peroxidase as tracers". Acta Anat (Basel) 153 (2): 135–44. doi:10.1159/000313647. PMID 8560966.
^ Lichtman JW, Purves D (1985). "Cell marking with horseradish peroxidase". Principles of neural development. Sunderland, Mass: Sinauer Associates. p. 114. ISBN 0-87893-744-7.

External links

Horseradish peroxidase at the US National Library of Medicine Medical Subject Headings (MeSH)

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The photoelectrochemical exploration of multifunctional TiO2 mesocrystals and its enzyme-assisted biosensing application.

Dai H1, Zhang S2, Gong L2, Li Y3, Xu G2, Lin Y4, Hong Z5.
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Mesocrystals, as the assemblies of crystallographically oriented nanocrystals, have single-crystal-like atom structures and scattering features but with much higher porosity than single-crystalline materials, making them promising substitutes for conventional single crystals in photoelectrochemical
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Human alpha-fetal protein immunoassay using fluorescence suppression with fluorescent-bead/antibody conjugate and enzymatic reaction.

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The aim of the study was to develop a simple and rapid immunoassay using fluorescent microbeads and enzyme-substrate reactions to measure alpha-fetal protein (AFP) concentrations. We demonstrated the functionality of the fluorescent immunosensor using antibody-conjugated fluorescent latex beads (AB-
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Japanese Journal

溶解および固定化した西洋ワサビペルオキシダーゼを用いるο-フェニレンジアミンから2,3-ジアミノフェナジンへの反応速度

諸岡成治,山中貴之,山崎吉一 [他]
福岡大学工学集報 = Fukuoka University review of technological sciences (89), 49-58, 2012-09
NAID 40019475115

Production of endothelial cell-enclosing alginate-based hydrogel fibers with a cell adhesive surface through simultaneous cross-linking by horseradish peroxidase-catalyzed reaction in a hydrodynamic spinning process

Liu Yang,Sakai Shinji,Taya Masahito
Journal of bioscience and bioengineering 114(3), 353-359, 2012-09
NAID 40019425004

Related Pictures

★リンクテーブル★

リンク元	「HRP」「西洋わさびペルオキシダーゼ」
拡張検索	「wheat germ agglutinin-horseradish peroxidase conjugate」
関連記事	「horseradish」「peroxidase」

「HRP」

Horseradish peroxidase
	Horseradish peroxidase C1
Identifiers
Organism	Armoracia rusticana
Symbol	Peroxidase C1A
Alt. symbols	PRXC1A
PDB	1GWU More structures
UniProt	P00433
Other data
EC number	1.11.1.7

　　[★]

西洋わさびペルオキシダーゼ

関: horseradish peroxidase

同: horseradish peroxidase

「西洋わさびペルオキシダーゼ」

　　[★]

英: horseradish peroxidase、HRP

「wheat germ agglutinin-horseradish peroxidase conjugate」

　　[★]

小麦胚芽凝集素・西洋ワサビペルオキシダーゼ複合体

「horseradish」

　　[★]

セイヨウワサビ、西洋わさび、ワサビダイコン、ホースラディッシュ

関: Armoracia rusticana

「peroxidase」

　　[★] ペルオキシダーゼ、過酸化酵素

関: myeloperoxidase

[pmid15641789-1] PDB 1w4y; Carlsson GH, Nicholls P, Svistunenko D, Berglund GI, Hajdu J (January 2005). "Complexes of horseradish peroxidase with formate, acetate, and carbon monoxide". Biochemistry 44 (2): 635–42. doi:10.1021/bi0483211. PMID 15641789.

[pmid9406554-2] PDB 1GWU; Gajhede M, Schuller DJ, Henriksen A, Smith AT, Poulos TL (December 1997). "Crystal structure of horseradish peroxidase C at 2.15 A resolution". Nat. Struct. Biol. 4 (12): 1032–8. doi:10.1038/nsb1297-1032. PMID 9406554.

[urlUniPDB-3] "Peroxidase C1A Related PDB sequences". UniPDB. European Bioinformatics Institute.

[pmid14751298-4] Veitch NC (February 2004). "Horseradish peroxidase: a modern view of a classic enzyme". Phytochemistry 65 (3): 249–59. doi:10.1016/j.phytochem.2003.10.022. PMID 14751298.

[Akkara-5] Akkara JA, Senecal KJ, Kaplan DL (October 1991). "Synthesis and characterization of polymers produced by horseradish peroxidase in dioxane". Journal of Polymer Science 29 (11): 1561–74. doi:10.1002/pola.1991.080291105.

[pmid8560966-6] Chau YP, Lu KS (1995). "Investigation of the blood-ganglion barrier properties in rat sympathetic ganglia by using lanthanum ion and horseradish peroxidase as tracers". Acta Anat (Basel) 153 (2): 135–44. doi:10.1159/000313647. PMID 8560966.

[isbn0-87893-744-7-7] Lichtman JW, Purves D (1985). "Cell marking with horseradish peroxidase". Principles of neural development. Sunderland, Mass: Sinauer Associates. p. 114. ISBN 0-87893-744-7.

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案内

案内

horseradish peroxidase