Salmonella enteritidis

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1. サルモネラ症の微生物学および疫学 microbiology and epidemiology of salmonellosis
2. 便培養で非チフス性サルモネラが検出された患者へのアプローチ approach to the patient with nontyphoidal salmonella in a stool culture
3. サルモネラ胃腸炎の病因 pathogenesis of salmonella gastroenteritis
4. 微生物による食中毒疾患の鑑別診断 differential diagnosis of microbial foodborne disease
5. 腸チフスの疫学、微生物学、臨床症状、および診断 epidemiology microbiology clinical manifestations and diagnosis of typhoid fever

English Journal

Lateral flow biosensor for DNA extraction-free detection of Salmonella based on aptamer mediated strand displacement amplification.

Fang Z1, Wu W1, Lu X1, Zeng L2.Author information 1Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.2Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China. Electronic address: zeng_lingwen@gibh.ac.cn.AbstractConvenient and sensitive point-of-care rapid diagnostic tests for food-borne pathogens have been a long-felt need of clinicians. Traditional approaches such as culture-based methods have good sensitivity and specificity, but they tend to be tedious and time-consuming. Herein we present a simple and sensitive aptamer based biosensor for rapid detection of Salmonella enteritidis (S. enteritidis). One of the aptamers specific for the outmembrane of S. enteritidis was used for magnetic bead enrichments. Another aptamer against S. enteritidis was used as a reporter for this pathogen, which was amplified by isothermal strand displacement amplification (SDA) and further detected by a lateral flow biosensor. As low as 10(1) colony forming unit (CFU) of S. enteritidis was detected in this study. Without DNA extraction, the reduced handling and simpler equipment requirement render this assay a simple and rapid alternative to conventional methods.
Biosensors & bioelectronics.Biosens Bioelectron.2014 Jun 15;56:192-7. doi: 10.1016/j.bios.2014.01.015. Epub 2014 Jan 17.
Convenient and sensitive point-of-care rapid diagnostic tests for food-borne pathogens have been a long-felt need of clinicians. Traditional approaches such as culture-based methods have good sensitivity and specificity, but they tend to be tedious and time-consuming. Herein we present a simple and
PMID 24491961

Neutralization of the antimicrobial effect of glyphosate by humic acid in vitro.

Shehata AA1, Kühnert M2, Haufe S2, Krüger M3.Author information 1Institute of Bacteriology and Mycology, Faculty of Veterinary Medicine, Leipzig University, An den Tierkliniken 29, 04103 Leipzig, Germany; Albrecht Daniel Thaer-Institute of Agronomy at the University Leipzig, Gustav-Kuhn Straße 8, 04159 Leipzig, Germany; Avian and Rabbit Diseases Department, Faculty of Veterinary Medicine, Sadat City University, Egypt. Electronic address: shehata@vetmed.uni-leipzig.de.2WHPharmawerk Weinböhla GmbH, Poststr. 58, 01689 Weinböhla, Germany.3Institute of Bacteriology and Mycology, Faculty of Veterinary Medicine, Leipzig University, An den Tierkliniken 29, 04103 Leipzig, Germany.AbstractIn the present study, the neutralization ability of the antimicrobial effect of glyphosate by different humic acids was investigated. The minimal inhibitory concentrations of glyphosate for different bacteria such as Bacillus badius, Bifidobacterium adolescentis, Escherichia coli, E. coli 1917 strain Nissle, Enterococcus faecalis, Enterococcus faecium, Salmonella enteritidis and Salmonella typhimurium were determined in the presence or absence of different concentrations of humic acid (0.25, 0.5 and 1.0mgmL(-1)). Our findings indicated that humic acids inhibited the antimicrobial effect of glyphosate on different bacteria. This information can help overcome the negative impact of glyphosate residues in feed and water.
Chemosphere.Chemosphere.2014 Jun;104:258-61. doi: 10.1016/j.chemosphere.2013.10.064. Epub 2013 Nov 20.
In the present study, the neutralization ability of the antimicrobial effect of glyphosate by different humic acids was investigated. The minimal inhibitory concentrations of glyphosate for different bacteria such as Bacillus badius, Bifidobacterium adolescentis, Escherichia coli, E. coli 1917 strai
PMID 24268342

Nicking enzyme-assisted biosensor for Salmonella enteritidis detection based on fluorescence resonance energy transfer.

Song Y1, Li W2, Duan Y1, Li Z1, Deng L3.Author information 1Department of Microbiology, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China.2Department of Microbiology, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China; College of Life Sciences, Central-South University, Changsha, Hunan 410008, China.3Department of Microbiology, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China. Electronic address: dengle@hunnu.edu.cn.AbstractSalmonella enteritidis (S. enteritidis) outbreaks continue to occur, and have increased public awareness of this pathogen. Nicking endonuclease Nb.BbvC I is widely used for the detection of biomolecules and displays activity for specific double-stranded DNA (dsDNA). In this study, we developed a biosensor to detect S. enteritidis based on fluorescence resonance energy transfer (FRET) using nicking enzyme and carbon nanoparticles (CNPs). Because of the quenching effect of black hole quencher 1 (BHQ 1), the CNPs do not fluoresce in the reaction system. When the target bacteria are added, the nicking enzyme recognizes and cleaves the dsDNA fabricated by the interaction between probe and target. As a result, the CNPs dissociate from BHQ 1 and emit strong fluorescence. Using the nicking enzyme, the fluorescence signals of the biosensor are greatly amplified. The biosensor exhibited a linear relationship with the concentration of S. enteritidis ranging from 10(2) to 3 × 10(3)CFU/mL in water and from 1.5 × 10(2) to 3 × 10(3)CFU/mL in milk. The present results indicate that our FRET-based detection system can be widely employed for the effective detection of pathogens.
Biosensors & bioelectronics.Biosens Bioelectron.2014 May 15;55:400-4. doi: 10.1016/j.bios.2013.12.053. Epub 2013 Dec 31.
Salmonella enteritidis (S. enteritidis) outbreaks continue to occur, and have increased public awareness of this pathogen. Nicking endonuclease Nb.BbvC I is widely used for the detection of biomolecules and displays activity for specific double-stranded DNA (dsDNA). In this study, we developed a bio
PMID 24434495

Japanese Journal

ひな白痢急速診断用菌液を用いた Salmonella Enteritidis 感染鶏の摘発と清浄化対策

武平有理子,野上真,橋村紘美
鶏病研究会報 47(2), 104-110, 2011-08-25
NAID 10029470926

市販台所用合成洗剤の細菌付着阻害および抗菌効果

宮本敬久,大石彬靖,河岸丈太郎,石橋明子,木下義将,目加田遥子,本城賢一
日本食品科学工学会誌 : Nippon shokuhin kagaku kogaku kaishi = Journal of the Japanese Society for Food Science and Technology 58(3), 127-130, 2011-03-15
… Enteritidis IFO 3313, P. … Enteritidis IFO 3313の付着は50％以上阻害されたが， P. …
NAID 10027868982