affinity electrophoresis

• アフィニティー電気泳動、親和電気泳動法

WordNet

1. a natural attraction or feeling of kinship; "an affinity for politics"; "the mysterious affinity between them"; "Jamess affinity with Sam"
2. inherent resemblance between persons or things
3. the force attracting atoms to each other and binding them together in a molecule; "basic dyes have an affinity for wool and silk" (同)chemical attraction
4. a close connection marked by community of interests or similarity in nature or character; "found a natural affinity with the immigrants"; "felt a deep kinship with the other students"; "anthropologys kinship with the humanities" (同)kinship
5. (biology) state of relationship between organisms or groups of organisms resulting in resemblance in structure or structural parts; "in anatomical structure prehistoric man shows close affinity with modern humans" (同)phylogenetic relation
6. (anthropology) kinship by marriage or adoption; not a blood relationship
7. (immunology) the attraction between an antigen and an antibody
8. the motion of charged particles in a colloid under the influence of an electric field; particles with a positive charge go to the cathode and negative to the anode (同)cataphoresis, dielectrolysis, ionophoresis

PrepTutorEJDIC

1. 〈C〉(人・物に対する)好み,親近感《+『for』(『to, with』)+『名』》 / 〈U〉〈C〉(2者の間の)密接な関係,類似《+『between』+『名』》;(…との)類縁《+『with』+『名』》 / 〈U〉〈C〉親和力(原子が化学反応を起こしやすい力)

Wikipedia preview

出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2015/12/14 09:37:59」(JST)

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[Wiki en表示]

The quantitative principle of affinity electrophoresis illustrated with electrophoresis at pH 8.6 of concanavalin A into an agarose gel containing blood serum (3.6 microliter per square cm). The bar indicates 1 cm. Electrophoresis performed overnight at less than 10 V/cm. The analysis was performed early in the 1970s at the Protein Laboratory

Affinity electrophoresis is a general name for many analytical methods used in biochemistry and biotechnology. Both qualitative and quantitative information may be obtained through affinity electrophoresis. The methods include the so-called mobility shift electrophoresis, charge shift electrophoresis and affinity capillary electrophoresis. The methods are based on changes in the electrophoretic pattern of molecules (mainly macromolecules) through biospecific interaction or complex formation. The interaction or binding of a molecule, charged or uncharged, will normally change the electrophoretic properties of a molecule. Membrane proteins may be identified by a shift in mobility induced by a charged detergent. Nucleic acids or nucleic acid fragments may be characterized by their affinity to other molecules. The methods has been used for estimation of binding constants, as for instance in lectin affinity electrophoresis or characterization of molecules with specific features like glycan content or ligand binding. For enzymes and other ligand-binding proteins, onedimensional electrophoresis similar to counter electrophoresis or to "rocket immunoelectrophoresis", affinity electrophoresis may be used as an alternative quantification of the protein. Some of the methods are similar to affinity chromatography by use of immobilized ligands.

See also

• Immunoelectrophoresis

External links

• Comprehensive texts edited by Niels H. Axelsen in Scandinavian Journal of Immunology, 1975 Volume 4 Supplement

UpToDate Contents

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• 1. 異常ヘモグロビン症の検査室診断 laboratory diagnosis of the hemoglobinopathies
• 2. 正常ヒトヘモグロビンの構造および機能 structure and function of normal human hemoglobins
• 3. ヘモグロビンの酸素親和性に関する遺伝性疾患 genetic disorders of hemoglobin oxygen affinity
• 4. ヘモグロビン変異の概要 introduction to hemoglobin mutations
• 5. 赤血球増加症患者に対する診断的アプローチ diagnostic approach to the patient with polycythemia

English Journal

• Effects of bile salts on propranolol distribution into liposomes studied by capillary electrophoresis.

• Yang L, Tucker IG, Ostergaard J.SourceSchool of Pharmacy, University of Otago, Dunedin, New Zealand.
• Journal of pharmaceutical and biomedical analysis.J Pharm Biomed Anal.2011 Nov 1;56(3):553-9. Epub 2011 Jul 2.
• The objective of this study was to study the effect of four different bile salts, cholate (C), deoxycholate (DC), taurocholate (TC), monoketocholate (MKC), on the membrane binding of a cationic model drug, propranolol, using capillary electrophoresis. The apparent distribution coefficient of propran
• PMID 21784594

• Characterization of glycation in an IgG1 by capillary electrophoresis sodium dodecyl sulfate and mass spectrometry.

• Kaschak T, Boyd D, Yan B.SourceDepartment of Pharma Technical Development, Genentech, Oceanside, CA 92056, USA.
• Analytical biochemistry.Anal Biochem.2011 Oct 15;417(2):256-63. Epub 2011 Jun 24.
• We report a case study of characterization of a non-enzymatically glycated IgG1 using reducing capillary electrophoresis sodium dodecyl sulfate (CE-SDS) and mass spectrometry (MS). Glycation was found to occur nonspecifically at multiple sites in both the light and heavy chains. The glycated light a
• PMID 21756870

Japanese Journal

• Detection of chymase-digested C-terminally truncated apolipoprotein A-I in normal human serum

• Usami Yoko,Matsuda Kazuyuki,Sugano Mitsutoshi,Ishimine Nau,Kurihara Yuriko,Sumida Tamaki,Yamauchi Kazuyoshi,Tozuka Minoru,山内 一由
• Journal of immunological methods 369(1-2), 51-58, 2011-06
• … A two-dimensional electrophoresis analysis also indicated that only two out of six spots that contained apoA-I fragments and had a molecular mass of 26 kDa after chymase digestion reacted with the 16–4 mAb. … We detected an extremely small amount of C-terminally truncated apoA-I in normal human serum by concentrating the serum through affinity chromatography using a 16–4 mAb-conjugated resin, and then performing Western blot analysis. …
• NAID 120003255801

• Planar Microfluidic System Based on Electrophoresis for Detection of 130-nm Magnetic Labels for Biosensing

• Takamura Tsukasa,Morimoto Yoshitaka,Sandhu Adarsh
• Jpn J Appl Phys 50(4), 04DL10-04DL10-3, 2011-04-25
• … For better biomolecular affinity and higher sensitivity, it is desirable to use sub-200-nm-diameter SPBs comparable in size to actual biomolecules. …
• NAID 150000055666

Related Links

• Affinity electrophoresis - Wikipedia, the free encyclopedia

Affinity electrophoresis is a general name for many analytical methods used in biochemistry and biotechnology. Both qualitative and quantitative information may be obtained through affinity electrophoresis. The methods include the so- called ...

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★リンクテーブル★

リンク元	「親和電気泳動法」「アフィニティー電気泳動」
関連記事	「affinity」「electrophoresis」

「親和電気泳動法」

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英

affinity electrophoresis

関

アフィニティー電気泳動

「アフィニティー電気泳動」

　　[★]

英

affinity electrophoresis

関

親和電気泳動法

「affinity」

　　[★]

同

親和性、アフィニティー

関

avidity

• 固有親和力
• 例えば、抗体の一方の抗原結合部位とB細胞エピトープとの間の平衡定数で表されるような親和性を指す

　 　 　 　 　 　 　 　

「electrophoresis」

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• 電気泳動、電気泳動法、泳動、泳動法

関

cataphoresis、electrophorese、electrophoretic、isotachophoresis、migration