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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2013/11/16 21:27:31」(JST)
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English Journal
- A single secreted luciferase-based gene reporter assay.
- Barriscale KA1, O'Sullivan SA1, McCarthy TV2.Author information 1School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland.2School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland. Electronic address: tmccarthy@ucc.ie.AbstractPromoter analysis typically employs a reporter gene fused to a test promoter combined with a second reporter fused to a control promoter that is used for normalization purposes. However, this approach is not valid when experimental conditions affect the control promoter. We have developed and validated a single secreted luciferase reporter (SSLR) assay for promoter analysis that avoids the use of a control reporter. The approach uses an early level of expression of a secreted luciferase linked to a test promoter as an internal normalization control for subsequent analysis of the same promoter. Comparison of the SSLR assay with the dual luciferase reporter (DLR) assay using HMGCR (3-hydroxy-3-methylglutaryl-coenzyme A reductase) and LDLR (low-density lipoprotein receptor) promoter constructs, which are down-regulated by 25-hydroxycholesterol, show that both assays yield similar results. Comparison of the response of the HMGCR promoter in SSLR transient assays compared very favorably with the response of the same promoter in the stable cell line. Overall, the SSLR assay proved to be a valid alternative to the DLR assay for certain applications and had significant advantages in that measurement of only one luciferase is required and monitoring can be continuous because cell lysis is not necessary.
- Analytical biochemistry.Anal Biochem.2014 May 15;453:44-9. doi: 10.1016/j.ab.2014.02.019. Epub 2014 Feb 28.
- Promoter analysis typically employs a reporter gene fused to a test promoter combined with a second reporter fused to a control promoter that is used for normalization purposes. However, this approach is not valid when experimental conditions affect the control promoter. We have developed and valida
- PMID 24583246
- The Alcohol Use Disorders Identification Test-Consumption (AUDIT-C) in the assessment of alcohol use disorders among acute injury patients.
- Wade D1, Varker T, Forbes D, O'Donnell M.Author information 1Australian Centre for Posttraumatic Mental Health, University of Melbourne, East Melbourne, Vic., Australia.AbstractBACKGROUND: The Alcohol Use Disorders Identification Test-Consumption (AUDIT-C) is a brief alcohol screening test and a candidate for inclusion in recommended screening and brief intervention protocols for acute injury patients. The objective of the current study was to examine the performance of the AUDIT-C to risk stratify injury patients with regard to their probability of having an alcohol use disorder.
- Alcoholism, clinical and experimental research.Alcohol Clin Exp Res.2014 Jan;38(1):294-9. doi: 10.1111/acer.12247. Epub 2013 Aug 22.
- BACKGROUND: The Alcohol Use Disorders Identification Test-Consumption (AUDIT-C) is a brief alcohol screening test and a candidate for inclusion in recommended screening and brief intervention protocols for acute injury patients. The objective of the current study was to examine the performance of th
- PMID 24033497
- Intestinal mucosal permeability of children with cefaclor-associated serum sickness-like reactions.
- Zhang Z1, Xiang Y, Wang B, Chen H, Cai X, Wang X, Mei L, Zheng Y.Author information 1Department of Laboratory Medicine, Wuhan Medical and Health Center for Women and Children, Tongji Medical College, Huazhong University of Science and Technology, 100 Xianggang Rd., Jiang An District, Wuhan, Hubei 430016, People's Republic of China.AbstractAlthough the serum sickness-like reaction (SSLR) in children after the administration of cefaclor has long been recognized, the exact mechanism of cefaclor-associated SSLR remains unclear. This study aims to investigate the association between intestinal mucosal permeability and cefaclor-associated SSLR in children. A total of 82 pediatric patients with upper respiratory tract infection following the cefaclor therapy was divided into cefaclor-associated SSLR positive group and negative group based on the presence or absence of SSLR after taking cefaclor, and 30 healthy volunteers served as control group. Urinary lactulose/mannitol (L/M) ratios and serum diamine oxidase (DAO) levels were determined in all cases on days 7, 9, 11, 13, and 15 after oral administration of cefaclor. The children in the control group were given the same measurements after enrollment in this study. From days 7 to 13, the urinary L/M ratio of children with cefaclor SSLR gradually increased and reached to the highest level of 0.38 ± 0.14 on day 13. Compared with the cefaclor-associated SSLR negative group and control group, urinary L/M ratios increased significantly in the cefaclor SSLR positive group on days 7, 9, 11, 13, and 15 after taking cefaclor, and serum levels of DAO following the treatment of cefaclor increased significantly in children with cefaclor SSLR on days 9, 11, 13, and 15. No significant difference in urinary L/M ratios and serum levels of DAO between SSLR negative group and control group through the entire experiment was observed. In conclusion, administration of cefaclor may induce SSLR in children by increasing the intestinal mucosal permeability and/or affecting the integrity of the intestinal mucosa. Determinations of urinary L/M ratios and serum DAO levels may be helpful for observing or predicting the occurrence of SSLR after administration of cefaclor, which will encourage physicians to proceed with extreme caution when prescribing cefaclor for pediatric patients.
- European journal of pediatrics.Eur J Pediatr.2013 Apr;172(4):537-43. doi: 10.1007/s00431-012-1926-y. Epub 2013 Jan 8.
- Although the serum sickness-like reaction (SSLR) in children after the administration of cefaclor has long been recognized, the exact mechanism of cefaclor-associated SSLR remains unclear. This study aims to investigate the association between intestinal mucosal permeability and cefaclor-associated
- PMID 23296953
Japanese Journal
- The Rapid Dementia Screening Test (RDST) (特集 軽度認知症をスクリーニングするための神経心理学的検査)
- 酒井 佳永
- 老年精神医学雑誌 21(2), 190-197, 2010-02
- NAID 40017010135
- 子どもの抑うつを測定する自己評価尺度の比較 : CDI, DSRS, CES-Dのカットオフ値に基づく判別精度
- 佐藤 寛,石川 信一,下津 咲絵 [他],佐藤 容子
- 児童青年精神医学とその近接領域 50(3), 307-317, 2009-06-01
- … Results of the SSLRs further demonstrated that these three scales were useful in screening for depressive disorders in Japanese community adolescents, applying the optimal cut-off points as noted.本研究の目的は,子どもの抑うつを測定する自己評価尺度の判別精度を受信者操作特性(ROC)分析と層別尤度比(SSLR)の観点から検討することであった。 … SSLRを算出したところ,CDIでは0-21点で0.51,22-30点で1.57,31-54点で108.40となった。 …
- NAID 10025341784
- Difference in mental symptoms between schizophrenia and depression: comparative study using a stratum-specific likelihood ratio
- Miyachi Yuka,Miyaoka Hitoshi,Tanaka Katsutoshi [他]
- The Kitasato medical journal 38(1), 46-51, 2008-03
- NAID 40015932490
Related Links
- Formålet med støtteforeningen er at bidrage til at skabe de bedst mulige rammer for medlemmerne i SLR og medlemmernes mulighed for at dyrke ridesporten på alle niveauer i Slangerup-Lynge Rideklub. Støtteforeningen for Slangerup ...
- SSLR stands for SonarSource Language Recognizer. It is a lightweight Java library which provides everything required to analyse any piece of source code. Using SSLR, you can quickly create a lexer, a parser, and some AST visitors ...
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★リンクテーブル★
| リンク元 | 「serum sickness-like reaction」 |
| 関連記事 | 「SS」 |
「serum sickness-like reaction」
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- SSLR