一本鎖DNA結合タンパク質

関: single-stranded DNA-binding protein

WordNet

any of a large group of nitrogenous organic compounds that are essential constituents of living cells; consist of polymers of amino acids; essential in the diet of animals for growth and for repair of tissues; can be obtained from meat and eggs and milk and legumes; "a diet high in protein"
the 19th letter of the Roman alphabet (同)s

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蛋白(たんばく)質
sulfurの化学記号 / {略}South[ern]

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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2013/02/03 23:14:57」(JST)

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Single-stranded DNA-binding protein, or SSB, binds to single-stranded regions of DNA to prevent premature annealing, to protect the single-stranded DNA from being digested by nucleases, and to remove secondary structure from the DNA to allow other enzymes to function effectively upon it. Single-stranded DNA is produced during all aspects of DNA metabolism: replication, recombination and repair. As well as stabilizing this single-stranded DNA, SSB proteins bind to and modulate the function of numerous proteins involved in all of these processes.

SSB proteins have been identified in organisms from viruses to humans. The only organisms known to lack them are Thermoproteales, a group of extremophile archaea, where they have been displaced by the protein ThermoDBP. While many phage and viral SSBs function as monomers and eukaryotes encode heterotrimeric RPA (Replication Protein A), the best characterized SSB is that from the bacteria E. coli which, like most bacterial SSBs exists as a tetramer. Active E. coli SSB is composed of four identical 19 kDa subunits. Binding of single-stranded DNA to the tetramer can occur in different "modes", with SSB occupying different numbers of DNA bases depending on a number of factors, including salt concentration. For example, the (SSB)₆₅ binding mode, in which approximately 65 nucleotides of DNA wrap around the SSB tetramer and contact all four of its subunits, is favoured at high salt concentrations in vitro. At lower salt concentrations, the (SSB)₃₅ binding mode, in which about 35 nucleotides bind to only two of the SSB subunits, tends to form. Further work is required to elucidate the functions of the various binding modes in vivo.

Viral SSB

Function

Herpes simple virus (HSV-1) single stranded DNA (ssDNA)-binding protein (SSB), ICP8, is a nuclear protein that, along other replication proteins is required for viral DNA replication during lytic infection.^[1]

Structure

The head consists of the eight alpha helices. The front side of the neck region consists of a five-stranded beta-sheet and two alpha helices whereas the back side is a three-stranded beta-sheet The shoulder part of the N-terminal domain contains an <alpha-helical and beta-sheet region.^[1]

Mechanism

Although the overall picture of Human cytomegalovirus (HHV-5) DNA synthesis appears typical of the Herpesviruses, some novel features are emerging. Six herpes virus-group-common genes encode proteins that likely constitute the replication fork machinery, including a two-subunit DNA polymerase, a helicas-primase complex and a single-stranded DNA-binding protein.^[2] The Human herpesvirus 1 (HHV-1) single-strand DNA-binding protein ICP8 is a 128kDa zinc metalloprotein. Photoaffinity labeling has shown that the region encompassing residues 368-902 contains the single-strand DNA-binding site of ICP8.^[3] The HHHV-1 UL5, UL8, and UL52 genes encode an essential heterotrimeric DNA helicase-primase that is responsible for concomitant DNA unwinding and primer synthesis at the viral DNA replication fork. ICP8 may stimulate DNA unwinding and enable bypass of cisplatin damaged DNA by recruiting the helicase-primase to the DNA.^[4]

Bacterial SSB

In molecular biology, SSB protein domain in bacteria are important in its function of maintaining DNA metabolism, more specifically DNA replication, repair and recombination.^[5] It has a structure of three beta-strands to a single six-stranded beta-sheet to form a dimer.^[6]

References

^ ^a ^b Mapelli M, Panjikar S, Tucker PA (2005). "The crystal structure of the herpes simplex virus 1 ssDNA-binding protein suggests the structural basis for flexible, cooperative single-stranded DNA binding.". J Biol Chem 280 (4): 2990–7. doi:10.1074/jbc.M406780200. PMID 15507432. http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=15507432.
^ Anders DG, McCue LA (1996). "The human cytomegalovirus genes and proteins required for DNA synthesis". Intervirology 39 (5-6): 378–88. PMID 9130047.
^ White EJ, Boehmer PE (October 1999). "Photoaffinity labeling of the herpes simplex virus type-1 single-strand DNA-binding protein (ICP8) with oligodeoxyribonucleotides". Biochem. Biophys. Res. Commun. 264 (2): 493–7. doi:10.1006/bbrc.1999.1566. PMID 10529391.
^ Tanguy Le Gac N, Villani G, Boehmer PE (May 1998). "Herpes simplex virus type-1 single-strand DNA-binding protein (ICP8) enhances the ability of the viral DNA helicase-primase to unwind cisplatin-modified DNA". J. Biol. Chem. 273 (22): 13801–7. PMID 9593724.
^ Meyer RR, Laine PS (December 1990). "The single-stranded DNA-binding protein of Escherichia coli". Microbiol. Rev. 54 (4): 342–80. PMC 372786. PMID 2087220. //www.ncbi.nlm.nih.gov/pmc/articles/PMC372786/.
^ Raghunathan S, Ricard CS, Lohman TM, Waksman G (June 1997). "Crystal structure of the homo-tetrameric DNA binding domain of Escherichia coli single-stranded DNA-binding protein determined by multiwavelength x-ray diffraction on the selenomethionyl protein at 2.9-A resolution". Proc. Natl. Acad. Sci. U.S.A. 94 (13): 6652–7. PMC 21213. PMID 9192620. //www.ncbi.nlm.nih.gov/pmc/articles/PMC21213/.

This article incorporates text from the public domain Pfam and InterPro IPR000635

External links

Single-Stranded+DNA+Binding+Proteins at the US National Library of Medicine Medical Subject Headings (MeSH)
SSB in PFAM

This protein-related article is a stub. You can help Wikipedia by expanding it.

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English Journal

Nucleotides sequestered at different subsite loci within DNA-binding pockets of two OB-fold single-stranded DNA-binding proteins are unstacked to different extents.

Nguyen HN, Zhao L, Gray CW, Gray DM, Xia T.SourceDepartment of Molecular and Cell Biology, University of Texas at Dallas, Richardson, TX, 75080.
Biopolymers.Biopolymers.2013 Jul;99(7):484-96. doi: 10.1002/bip.22204.
The gene 5 protein (g5p) encoded by the Ff strains of Escherichia coli bacteriophages is a dimeric single-stranded DNA-binding protein (SSB) that consists of two identical OB-fold (oligonucleotide/oligosaccharide-binding) motifs. Ultrafast time-resolved fluorescence measurements were carried out to
PMID 23616216

DNA-Damaging Agents Induce the RecA-Independent Homologous Recombination Functions of Integrating Conjugative Elements of the SXT/R391 Family.

Garriss G, Poulin-Laprade D, Burrus V.SourceDépartement de Biologie, Université de Sherbrooke, Sherbrooke, Québec, Canada.
Journal of bacteriology.J Bacteriol.2013 May;195(9):1991-2003. doi: 10.1128/JB.02090-12. Epub 2013 Feb 22.
Integrating conjugative elements (ICEs) of the SXT/R391 family are major contributors to the spread of antibiotic resistance genes. These elements also catalyze their own diversity by promoting inter-ICE recombination through the action of the RecA-independent homologous recombination system that th
PMID 23435980

Japanese Journal

Basic and aromatic residues in the C-terminal domain of PriC are involved in ssDNA and SSB binding

The journal of biochemistry 157(6), 529-537, 2015-06
NAID 40020489279

血管性浮腫を伴った低補体血症性蕁麻疹様血管炎症候群の1例

皮膚の科学 14(3), 123-129, 2015
NAID 130005103409

C-terminal region of activation-induced cytidine deaminase (AID) is required for efficient class switch recombination and gene conversion.

Proceedings of the National Academy of Sciences of the United States of America 111(6), 2253-2258, 2014-02-11
NAID 120005395696

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★リンクテーブル★

リンク元	「一本鎖DNA結合タンパク質」「single-stranded DNA-binding protein」
関連記事	「SS」「SSB」「S」

「一本鎖DNA結合タンパク質」

SSB
	Crystal structure of PriB- a primosomal DNA replication protein of Escherichia coli
Identifiers
Symbol	SSB
Pfam	PF00436
Pfam clan	CL0021
InterPro	IPR000424
PROSITE	PDOC00602
SCOP	1kaw
SUPERFAMILY	1kaw
TCDB	3.A.7
Available protein structures:
Pfam	structures
PDB	RCSB PDB; PDBe
PDBsum	structure summary

Viral_DNA_bp
	Single Stranded DNA-binding protein(icp8) from Herpes simplex virus-1
Identifiers
Symbol	Viral_DNA_bp
Pfam	PF00747
InterPro	IPR000635
Available protein structures:
Pfam	structures
PDB	RCSB PDB; PDBe
PDBsum	structure summary

　　[★]

英: single-stranded DNA-binding protein、SSB protein
同: SSB蛋白質、一本鎖DNA結合タンパク、一本鎖DNA結合蛋白、一本鎖DNA結合蛋白質
関: DNA結合タンパク質

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一本鎖DNA結合タンパク : 27 件
一本鎖DNA結合蛋白 : 9 件
一本鎖DNA結合タンパク質 : 73 件
一本鎖DNA結合蛋白質 : 34 件

「single-stranded DNA-binding protein」

　　[★]

一本鎖DNA結合タンパク質

関: DNA-binding protein、SSB protein

「SS」

　　[★]

「SSB」

　　[★]

SSB protein：一本鎖DNA結合タンパク質 single-stranded DNA-binding protein

「S」

　　[★]

[pmid15507432-1] Mapelli M, Panjikar S, Tucker PA (2005). "The crystal structure of the herpes simplex virus 1 ssDNA-binding protein suggests the structural basis for flexible, cooperative single-stranded DNA binding.". J Biol Chem 280 (4): 2990–7. doi:10.1074/jbc.M406780200. PMID 15507432. http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=15507432.

[pmid9130047-2] Anders DG, McCue LA (1996). "The human cytomegalovirus genes and proteins required for DNA synthesis". Intervirology 39 (5-6): 378–88. PMID 9130047.

[pmid10529391-3] White EJ, Boehmer PE (October 1999). "Photoaffinity labeling of the herpes simplex virus type-1 single-strand DNA-binding protein (ICP8) with oligodeoxyribonucleotides". Biochem. Biophys. Res. Commun. 264 (2): 493–7. doi:10.1006/bbrc.1999.1566. PMID 10529391.

[pmid9593724-4] Tanguy Le Gac N, Villani G, Boehmer PE (May 1998). "Herpes simplex virus type-1 single-strand DNA-binding protein (ICP8) enhances the ability of the viral DNA helicase-primase to unwind cisplatin-modified DNA". J. Biol. Chem. 273 (22): 13801–7. PMID 9593724.

[pmid2087220-5] Meyer RR, Laine PS (December 1990). "The single-stranded DNA-binding protein of Escherichia coli". Microbiol. Rev. 54 (4): 342–80. PMC 372786. PMID 2087220. //www.ncbi.nlm.nih.gov/pmc/articles/PMC372786/.

[pmid9192620-6] Raghunathan S, Ricard CS, Lohman TM, Waksman G (June 1997). "Crystal structure of the homo-tetrameric DNA binding domain of Escherichia coli single-stranded DNA-binding protein determined by multiwavelength x-ray diffraction on the selenomethionyl protein at 2.9-A resolution". Proc. Natl. Acad. Sci. U.S.A. 94 (13): 6652–7. PMC 21213. PMID 9192620. //www.ncbi.nlm.nih.gov/pmc/articles/PMC21213/.

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SSB protein