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1. HIV-1 RNA定量の技術および解釈 techniques and interpretation of hiv 1 rna quantitation
2. 分子遺伝学の原理 principles of molecular genetics
3. 遺伝学およびゲノミクスのためのツール：遺伝子発現プロファイリング tools for genetics and genomics gene expression profiling
4. 慢性C型肝炎ウイルス感染の診断および評価 diagnosis and evaluation of chronic hepatitis c virus infection
5. 遺伝性疾患の基本原則 basic principles of genetic disease

Divalent ions attenuate DNA synthesis by human DNA polymerase α by changing the structure of the template/primer or by perturbing the polymerase reaction.

Zhang Y1, Baranovskiy AG2, Tahirov ET2, Tahirov TH3, Pavlov YI4.
DNA repair.DNA Repair (Amst).2016 Jul;43:24-33. doi: 10.1016/j.dnarep.2016.05.017. Epub 2016 May 12.
DNA polymerases (pols) are sophisticated protein machines operating in the replication, repair and recombination of genetic material in the complex environment of the cell. DNA pol reactions require at least two divalent metal ions for the phosphodiester bond formation. We explore two understudied r
PMID 27235627

The yeast mitochondrial RNA polymerase and transcription factor complex catalyzes efficient priming of DNA synthesis on single-stranded DNA.

Ramachandran A1, Nandakumar D1, Deshpande A1, Lucas TP2, Bhojappa RR3, Tang GQ1, Raney K3, Yin YW2, Patel SS4.
The Journal of biological chemistry.J Biol Chem.2016 Jun 16. pii: jbc.M116.740282. [Epub ahead of print]
Primases use single-stranded (ss) DNAs as templates to synthesize short oligoribonucleotide primers that initiate lagging strand DNA synthesis or reprime DNA synthesis after replication fork collapse, but the origin of this activity in the mitochondria remains unclear. Herein, we show that the Sacch
PMID 27311715

Primer release is the rate-limiting event in lagging-strand synthesis mediated by the T7 replisome.

Hernandez AJ1, Lee SJ1, Richardson CC2.
Proceedings of the National Academy of Sciences of the United States of America.Proc Natl Acad Sci U S A.2016 May 24;113(21):5916-21. doi: 10.1073/pnas.1604894113. Epub 2016 May 9.
DNA replication occurs semidiscontinuously due to the antiparallel DNA strands and polarity of enzymatic DNA synthesis. Although the leading strand is synthesized continuously, the lagging strand is synthesized in small segments designated Okazaki fragments. Lagging-strand synthesis is a complex eve
PMID 27162371

.BETA.-Sitosteryl (6'-O-linoleoyl)-glucoside of Soybean (Glycine max L.) Crude Extract Inhibits Y-Family DNA Polymerases

Horie Sho,Okuda Chiaki,Yamashita Takatoshi [他],Watanabe Kenichi,Sato Yoshihiro,Yamaguchi Yasuhiro,Takeuchi Toshifumi,Sugawara Fumio,Yoshida Hiromi,Mizushina Yoshiyuki
Journal of Oleo Science 59(11), 621-630, 2010
… In addition, compound 1 had no effect on prokaryotic pols or other DNA metabolic enzymes such as calf primase of pol α, T7 RNA polymerase, T4 polynucleotide kinase, or bovine deoxyribonuclease I. …
NAID 130000437428