関: myosin light chain kinase

PrepTutorEJDIC

Medieval Latin

Wikipedia preview

出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2016/12/16 19:15:30」(JST)

wiki en

Human Myosin Light-Chain Kinase
The Crystal Structure of the Human Myosin Light Chain Kinase Loc340156.^[2]
Identifiers
Symbol	MYLK4
Entrez	340156
HUGO	27972
RefSeq	NM_001012418
UniProt	Q86YV6

Myosin light-chain kinase also known as MYLK or MLCK is a serine/threonine-specific protein kinase that phosphorylates a specific myosin light chain, namely, the regulatory light chain of myosin II.^[3]

Isoforms

Four different MLCK isoforms exist:^[4]

MYLK – smooth muscle
MYLK2 – skeletal
MYLK3 – cardiac
MYLK4 – novel

Function

These enzymes are important in the mechanism of contraction in muscle. Once there is an influx of calcium cations (Ca²⁺) into the muscle, either from the sarcoplasmic reticulum or from the extracellular space, contraction of smooth muscle fibres may begin. First, the calcium will bind to calmodulin. This binding will activate MLCK, which will go on to phosphorylate the myosin light chain at serine residue 19. This will enable the myosin crossbridge to bind to the actin filament and allow contraction to begin (through the crossbridge cycle). Since smooth muscle does not contain a troponin complex, as striated muscle does, this mechanism is the main pathway for regulating smooth muscle contraction. Reducing intracellular calcium concentration inactivates MLCK but does not stop smooth muscle contraction since the myosin light chain has been physically modified through phosphorylation(and not via ATPase activity). To stop smooth muscle contraction this change needs to be reversed. Dephosphorylation of the myosin light chain (and subsequent termination of muscle contraction) occurs through activity of a second enzyme known as myosin light-chain phosphatase (MLCP).

References

^ "RCSB Protein Data Bank - Structure Summary for 3KF9 - Crystal structure of the SdCen/skMLCK complex".
^ "RCSB Protein Data Bank - Structure Summary for 2X4F - The Crystal Structure of the Human Myosin Light Chain Kinase Loc340156.".
^ Gao Y, Ye LH, Kishi H, Okagaki T, Samizo K, Nakamura A, Kohama K (June 2001). "Myosin light chain kinase as a multifunctional regulatory protein of smooth muscle contraction". IUBMB Life. 51 (6): 337–44. doi:10.1080/152165401753366087. PMID 11758800.
^ Manning G, Whyte DB, Martinez R, Hunter T, Sudarsanam S (December 2002). "The protein kinase complement of the human genome". Science. 298 (5600): 1912–34. doi:10.1126/science.1075762. PMID 12471243.

External links

MYLK protein, human at the US National Library of Medicine Medical Subject Headings (MeSH)
Myosin-Light-Chain Kinase at the US National Library of Medicine Medical Subject Headings (MeSH)

This article incorporates text from the United States National Library of Medicine, which is in the public domain.

English Journal

L-NAME improves doxycycline and ML-7 cardioprotection from oxidative stress.

Bil-Lula I1, Krzywonos-Zawadzka A1, Sawicka J2, Bialy D3, Wawrzynska M4, Wozniak M5, Sawicki G6.
Frontiers in bioscience (Landmark edition).Front Biosci (Landmark Ed).2018 Jan 1;23:298-309.
PMID 28930548

Carbonic anhydrase inhibitor acetazolamide shifts synaptic vesicle recycling to a fast mode at the mouse neuromuscular junction.

Bertone NI1, Groisman AI1, Mazzone GL2, Cano R3, Tabares L3, Uchitel OD1.
Synapse (New York, N.Y.).Synapse.2017 Dec;71(12). doi: 10.1002/syn.22009. Epub 2017 Sep 21.
PMID 28873252

The signaling of protease-activated receptor-2 activating peptide-induced contraction in cat esophageal smooth muscle cells.

Ha HS1, Lee SE1, Lee HS1, Kim GH1, Yoon CJ1, Han JS1, Lee JY2, Sohn UD3.
Archives of pharmacal research.Arch Pharm Res.2017 Nov 2. doi: 10.1007/s12272-017-0975-1. [Epub ahead of print]
PMID 29098568

Japanese Journal

Induction of myosin light chain kinase and CPI-17 by TGF-β accelerates contractile activity in intestinal epithelial cells

Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ, 2018
NAID 130006725616

Hydrogen sulfide donor NaHS induces death of alveolar epithelial L2 cells that is associated with cellular shrinkage, transgelin expression and myosin phosphorylation

Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｔｏｘｉｃｏｌｏｇｉｃａｌ　Ｓｃｉｅｎｃｅｓ 41(5), 645-654, 2016
NAID 130005266228

Endothelin-1, but not angiotensin II, induces afferent arteriolar myosin diphosphorylation as a potential contributor to prolonged vasoconstriction

Kidney International 87(2), 370-381, 2015-02
NAID 120005728501

Related Pictures

★リンクテーブル★

リンク元	「ミオシン軽鎖キナーゼ」
関連記事	「ML」「MLC」

「ミオシン軽鎖キナーゼ」

myosin light-chain kinase 3, cardiac
Identifiers
Symbol	MYLK3
Entrez	91807
HUGO	29826
OMIM	612147
RefSeq	NM_182493
UniProt	Q32MK0
Other data
Locus	Chr. 16 q11.2

myosin light-chain kinase 2, skeletal muscle
	Crystal structure of the S. dubia centrin / human skeletal muscle myosin light-chain complex.
Identifiers
Symbol	MYLK2
Entrez	85366
HUGO	16243
OMIM	606566
RefSeq	NM_033118
UniProt	Q9H1R3
Other data
Locus	Chr. 20 q13.31

myosin light-polypeptide kinase, smooth muscle
Identifiers
Symbol	MYLK
Entrez	4638
HUGO	7590
OMIM	600922
RefSeq	NM_053025
UniProt	Q15746
Other data
EC number	2.7.11.18
Locus	Chr. 3 qcen-q21

　　[★]

英: myosin light chain kinase, MLCK
同: ミオシンL鎖キナーゼII
関: ミオシン軽鎖

「ML」

　　[★]

「MLC」

　　[★]

[3KF9-1] "RCSB Protein Data Bank - Structure Summary for 3KF9 - Crystal structure of the SdCen/skMLCK complex".

[2X4F-2] "RCSB Protein Data Bank - Structure Summary for 2X4F - The Crystal Structure of the Human Myosin Light Chain Kinase Loc340156.".

[pmid11758800-3] Gao Y, Ye LH, Kishi H, Okagaki T, Samizo K, Nakamura A, Kohama K (June 2001). "Myosin light chain kinase as a multifunctional regulatory protein of smooth muscle contraction". IUBMB Life. 51 (6): 337–44. doi:10.1080/152165401753366087. PMID 11758800.

[pmid12471243-4] Manning G, Whyte DB, Martinez R, Hunter T, Sudarsanam S (December 2002). "The protein kinase complement of the human genome". Science. 298 (5600): 1912–34. doi:10.1126/science.1075762. PMID 12471243.

v t e Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

匿名

検索

案内

案内

MLCK