同: erythrocyte glutatuione reductase

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the 5th letter of the Roman alphabet (同)e

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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2014/06/15 23:26:16」(JST)

English Journal

Dynamic encapsulation of hydrophilic nisin in hydrophobic poly (lactic acid) particles with controlled morphology by a single emulsion process.

Ji S1, Lu J2, Liu Z3, Srivastava D1, Song A1, Liu Y3, Lee I4.Author information 1Department of Chemical Engineering and Materials Science, Michigan State University, 2527 Engineering Building, East Lansing, MI 48824, USA.2Metna Co., 1926 Turner Street, Lansing, MI 48906, USA.3Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA.4Department of Chemical Engineering and Materials Science, Michigan State University, 2527 Engineering Building, East Lansing, MI 48824, USA. Electronic address: leeil@egr.msu.edu.AbstractHydrophilic nisin-loaded hydrophobic poly (lactic acid) (PLA) particles with controlled size and shape were successfully produced utilizing a one-step single emulsification method. Preliminary shear stress and temperature tests showed that there was no significant loss in the nisin inhibition activity during this process. PLA/nisin composite particles were prepared into solid nanocomposite spheres (50-200nm) or hollow microcomposite spheres (1-5μm) under the operative conditions developed in our previous study, in which the hydrophilic nisin in the aqueous phase solution could be entrapped in the hydrophobic polymer in the emulsification process generating either single or multiple emulsions. The incorporation of nisin in PLA had little effect on key processing conditions, which allows the dynamic control of the morphology and property of resulting particles. Microscopic and surface analyses suggested that nisin was dispersed uniformly inside the polymer matrix and adsorbed on the particle surface. The encapsulation amount and efficiency were enhanced with the increase in nisin loading in the aqueous solution. Unique reversible control of particle size and shape by this process was successfully applied in the nisin encapsulation. Alternating temperature in the repeating emulsification steps improved the encapsulation efficiency while generated particles in desired size and shape.
Journal of colloid and interface science.J Colloid Interface Sci.2014 Jun 1;423:85-93. doi: 10.1016/j.jcis.2014.02.025. Epub 2014 Mar 3.
Hydrophilic nisin-loaded hydrophobic poly (lactic acid) (PLA) particles with controlled size and shape were successfully produced utilizing a one-step single emulsification method. Preliminary shear stress and temperature tests showed that there was no significant loss in the nisin inhibition activi
PMID 24703672

Expression, purification and characterization of a functional carbohydrate-binding module from Streptomyces sp. SirexAA-E.

Lim S1, Chundawat SP2, Fox BG3.Author information 1Department of Biochemistry, University of Wisconsin, Madison, WI 53706, United States.2Department of Biochemistry, University of Wisconsin, Madison, WI 53706, United States; DOE Great Lakes Bioenergy Research Center (GLBRC), Madison, WI 53703, United States; Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI 48824, United States. Electronic address: chundawa@egr.msu.edu.3Department of Biochemistry, University of Wisconsin, Madison, WI 53706, United States; DOE Great Lakes Bioenergy Research Center (GLBRC), Madison, WI 53703, United States. Electronic address: bgfox@biochem.wisc.edu.AbstractStreptomyces sp. SirexAA-E (ActE) has been identified as a highly cellulolytic actinobacterium capable of deconstructing lignocellulosic biomass. SirexAA-E CAZymes most frequently contain a carbohydrate-binding module from family 2a (CBM2a). The DNA encoding the CBM2a from gene locus SACTE_0237, the most abundantly expressed cellulase from SirexAA-E, was cloned into an Escherichia coli expression vector and expressed as a C-terminal fusion protein to GFP. The GFP-CBM2a fusion protein was purified from insoluble inclusion bodies and refolded. The solubilized protein was separated by size-exclusion chromatography into high molecular weight GFP-CBM2a multimers and monomeric GFP-CBM2a. Only the monomeric CBM2a protein was found to have high relative affinity (partition coefficient of 0.62±0.04L/g) to cellulose. Binding of monomeric CBM2a prepared in this manner exhibits fully reversible, high affinity binding to cellulose.
Protein expression and purification.Protein Expr Purif.2014 Jun;98:1-9. doi: 10.1016/j.pep.2014.02.013. Epub 2014 Mar 7.
Streptomyces sp. SirexAA-E (ActE) has been identified as a highly cellulolytic actinobacterium capable of deconstructing lignocellulosic biomass. SirexAA-E CAZymes most frequently contain a carbohydrate-binding module from family 2a (CBM2a). The DNA encoding the CBM2a from gene locus SACTE_0237, the
PMID 24607362

Fetal hypoxia increases vulnerability of hypoxic-ischemic brain injury in neonatal rats: Role of glucocorticoid receptors.

Gonzalez-Rodriguez PJ1, Xiong F1, Li Y1, Zhou J1, Zhang L2.Author information 1Center for Perinatal Biology, Division of Pharmacology, Department of Basic Sciences, Loma Linda University School of Medicine, Loma Linda, CA 92350, USA.2Center for Perinatal Biology, Division of Pharmacology, Department of Basic Sciences, Loma Linda University School of Medicine, Loma Linda, CA 92350, USA. Electronic address: lzhang@llu.edu.AbstractGestational hypoxia is a common stress to the fetal development and increases the risk of neonatal morbidity. The present study tested the hypothesis that fetal hypoxia results in heightened brain vulnerability to hypoxic-ischemic (HI) injury in neonatal rats via down-regulation of glucocorticoid receptor (GR) in the developing brain. Time-dated pregnant rats were exposed to hypoxia (10.5% O2) from days 15 to 21 of gestation. Brain HI injury was determined in day 10 pups. Maternal hypoxia resulted in asymmetric intrauterine growth restriction in the fetus. The brain HI injury was significantly increased in maternal hypoxia-treated pups as compared with the normoxia control in both males and females. Activation of brain GR by dexamethasone injection into the right lateral ventricle produced a concentration-dependent reduction of HI-induced brain injury in control pups. Maternal hypoxia significantly decreased GR mRNA and protein abundance in the fetal brain and neonatal hippocampus and abolished the dexamethasone-mediated neuroprotective effect in pup brains. This decreased GR expression was resulted from increased DNA methylation, decreased binding of transcription factors Egr-1 and Sp1 to GR gene exon 17 and 111 promoters, and reduced expression of GR exon 17 and 111 mRNA variants. The results demonstrate that gestational hypoxia causes epigenetic repression of GR gene expression in the developing brain resulting in the heightened brain vulnerability to HI injury in neonatal rats.
Neurobiology of disease.Neurobiol Dis.2014 May;65:172-9. doi: 10.1016/j.nbd.2014.01.020. Epub 2014 Feb 7.
Gestational hypoxia is a common stress to the fetal development and increases the risk of neonatal morbidity. The present study tested the hypothesis that fetal hypoxia results in heightened brain vulnerability to hypoxic-ischemic (HI) injury in neonatal rats via down-regulation of glucocorticoid re
PMID 24513088

Japanese Journal

実機排ガスを含むメタン-空気混合気の層流燃焼速度の測定 : 第2報.層流燃焼速度に及ぼすEGRの効果

菊池祐輔,田中淳弥
工学院大学研究報告 (118), 73-78, 2015-04-30
… In thissecond report, the effect of EGR gas on laminar burning velocity is evaluated by using of Bunsen burner method. … Theexperiment data show that laminar burning velocity is capable of keeping sufficiently high within 10% EGR gas, then italso show the temperature of flame can be kept low to keep the engine performance and to suppress the NOx emission.In the case of mixing exhaust gas from the real engine, the unburnt fuel strongly affects laminar burning velocity. …
NAID 110009896025

実機排ガスを含むメタン-空気混合気の層流燃焼速度の測定 : 第1報.測定装置の基本特性

菊池祐輔,田中淳弥
工学院大学研究報告 (118), 67-71, 2015-04-30
… The exhaust gas recirculation (EGR) is widely used for commercial spark ignition engine not only to reduce emissionbut also to reduce pumping loss at partial load. … On the other hand, the high EGR rate with partial load operationdeteriorates the emission and engine output. … However, it is difficult to show the critical rate of EGR by the engine test.This is because each engine is designed in various ways for the commercial use. …
NAID 110009896024

実機排ガスを含むメタン-空気混合気の層流燃焼速度の測定(第2報)層流燃焼速度に及ぼすEGRの効果

菊池祐輔,田中淳弥
工学院大学研究報告 = Research reports of Kogakuin University (118), 73-78, 2015-04
NAID 40020472645

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