DEAE-デキストラン
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English Journal
- Non-viral transfection methods optimized for gene delivery to a lung cancer cell line.
- Salimzadeh L, Jaberipour M, Hosseini A, Ghaderi A.SourceShiraz Institute for Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran.
- Avicenna journal of medical biotechnology.Avicenna J Med Biotechnol.2013 Apr;5(2):68-77.
- BACKGROUND: Mehr-80 is a newly established adherent human large cell lung cancer cell line that has not been transfected until now. This study aims to define the optimal transfection conditions and effects of some critical elements for enhancing gene delivery to this cell line by utilizing different
- PMID 23799175
- Optimization of the transductional efficiency of lentiviral vectors: effect of sera and polycations.
- Denning W, Das S, Guo S, Xu J, Kappes JC, Hel Z.SourceDepartment of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294-2182, USA.
- Molecular biotechnology.Mol Biotechnol.2013 Mar;53(3):308-14. doi: 10.1007/s12033-012-9528-5.
- Lentiviral vectors are widely used as effective gene-delivery vehicles. Optimization of the conditions for efficient lentiviral transduction is of a high importance for a variety of research applications. Presence of positively charged polycations reduces the electrostatic repulsion forces between a
- PMID 22407723
- Biochemical properties and comparative pharmacology of a coagulant from Deinagkistrodon acutus snake venom.
- Tang SS, Wang XH, Zhang JH, Tang BS, Qian L, Li PY, Luo LW.SourceGuangdong Key Laboratory of Pharmaceutical Bioactive Substances, School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou 510006, China. Electronic address: songstang@gmail.com.
- European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences.Eur J Pharm Sci.2013 Feb 18;49(1):90-98. doi: 10.1016/j.ejps.2013.02.002. [Epub ahead of print]
- A number of snake venom thrombin-like enzymes (TLEs) have already been characterized. Some TLEs play significant roles in vessel injury hemostasis. A novel TLE (Agacutase) was purified from Deinagkistrodon acutus snake venom by the means of Sephadex G-75, DEAE-Sepharose FF, and Sephadex G-25 column
- PMID 23429184
Japanese Journal
- DEAE-デキストラン共重合体を非ウイルス性遺伝子導入キャリアーとして用いた、培養細胞への外来遺伝子導入
- 江下 優樹,東原 絢子,大西 政康,水野 正明,吉田 純,住吉 秀明,吉岡 秀克,高崎 智彦,石川 雄一,久保田 直治,大西 靖彦,エシタ ユウキ,ヒガシハラ ジュンコ,オオニシ マサヤス,ミズノ マサアキ,ヨシダ ジュン,スミヨシ ヒデアキ,ヨシオカ ヒデカツ,タカサキ トシヒコ,イシカワ ユウイチ,クボタ ナオジ,オオニシ ヤスヒコ,Eshita Yuki,Higashihara Junko,Onishi Masayasu,Mizuno Masaaki,Yoshida Jun,Sumiyoshi Hideaki,Yoshioka Hidekatsu,Takasaki Toshihiko,Ishikawa Yuichi,Kubota Naoji,Onishi Yasuhiko
- 大分大学工学部研究報告 57, 1-8, 2009
- … Transfection efficiency of 2-diethyl aminoethyl-dextran-methyl methacrylate graft copolymer (DDMC) was evaluated with pGL3 DNA and COS-7 cells. … Results were as follows: 1) When DNA (0.05 ㎍/well) and DDMC (4.0 ㎍/well) were transfected into the cells, RLU was about double higher than DNA and DEAE-dextran. … 2) The first peak of RLU on DDMC and DEAE-dextran appeared at 72 hours and 48 hours after the transfection, respectively. …
- NAID 120002187927
- 15 新規高分子DEAE-デキストラン共重合体を非ウイルス性遺伝子導入キャリアーとして用いた,培養細胞への外来遺伝子導入(一般講演,第55回日本衛生動物学会南日本支部大会講演要旨)
- 東原 絢子,大西 靖彦,水野 正明,吉田 純,多森 直樹,Dieng H.,加藤 幸太郎,岡田 貴志,江下 優樹
- 衞生動物 57(2), 175, 2006-06-15
- NAID 110004799650
- 2-Diethylaminoethyl(DEAE)-Dextran-MMA Graft Copolymer for Non-Viral Gene Delivery
- Onishi Yasuhiko,Eshita Yuki,Murashita Aya [他]
- 日本文理大学環境科学研究所報告 (5), 8-13, 2006
- NAID 40015297832
Related Links
- Sigma-Aldrich offers Sigma-DEAE-Dextran hydrochloride for your research needs. Find product specific information, including CAS, MSDS, protocols and references.
- DEAE-Dextran (DEAE-D) is also used for transfecting animal cells with foreign DNA. It is added to solution containing DNA meant for transfection. It binds and interacts with negatively charged DNA molecules and via a largely unknown ...
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