WordNet
- a segment of DNA that can become integrated at many different sites along a chromosome (especially a segment of bacterial DNA that can be translocated as a whole) (同)jumping gene
- an event capable of causing a mutation
Wikipedia preview
出典(authority):フリー百科事典『ウィキペディア(Wikipedia)』「2013/01/22 16:55:58」(JST)
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Transposon mutagenesis, or transposition mutagenesis, is a biological process that allows genes to be transferred to a host organism's chromosome, interrupting or modifying the function of an extant gene on the chromosome and causing mutation.[1]
Contents
- 1 History
- 2 Dynamics
- 3 See also
- 4 References
- 5 External links
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History
Transposon mutagenesis was first studied by Barbara McClintock in the mid-20th century during her Nobel Prize-winning work with corn.[2][3]
Dynamics
In the case of bacteria, transposition mutagenesis is usually accomplished by way of a plasmid from which a transposon is extracted and inserted into the host chromosome. This usually requires a set of enzymes including transposase to be translated.[3]
See also
- Transposons as a genetic tool
References
- ^ "Feeding hungry mouths" - European Commission
- ^ "Detecting a transposon in corn" - Weed to Wonder
- ^ a b "Detecting a Transposon Tag in Arabidopsis" - Greenomes
External links
- GMO Safety: Jumping genes - from phenomenon to tool
- "Transposon Mutagenisis" - Food Ingredient News
- "Pathogenesis and Immunity in Pertussis" - JAMA
- "Transposon mutagenesis of nuclear photosynthetic genes in Zea mays" - SpringerLink
UpToDate Contents
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English Journal
- Identifying novel mycobacterial stress associated genes using a random mutagenesis screen in Mycobacterium smegmatis.
- Viswanathan G1, Joshi SV1, Sridhar A1, Dutta S1, Raghunand TR2.
- Gene.Gene.2015 Dec 10;574(1):20-7. doi: 10.1016/j.gene.2015.07.063. Epub 2015 Jul 23.
- Cell envelope associated components of Mycobacterium tuberculosis (M.tb) have been implicated in stress response, immune modulation and in vivo survival of the pathogen. Although many such factors have been identified, there is a large disparity between the number of genes predicted to be involved i
- PMID 26211627
- Buckle up! Transposon mutagenesis can differentiate melanoma drivers from their many passengers.
- Farthing HM, Marie KL, Merlino G1.
- Pigment cell & melanoma research.Pigment Cell Melanoma Res.2015 Nov;28(6):646-7. doi: 10.1111/pcmr.12416.
- PMID 26314840
- Random transposon mutagenesis of the Saccharopolyspora erythraea genome reveals additional genes influencing erythromycin biosynthesis.
- Fedashchin A1, Cernota WH1, Gonzalez MC1, Leach BI1, Kwan N1, Wesley RK1, Weber JM2.
- FEMS microbiology letters.FEMS Microbiol Lett.2015 Oct 13. pii: fnv180. [Epub ahead of print]
- A single cycle of strain improvement was performed in Saccharopolyspora erythraea mutB and 15 genotypes influencing erythromycin production were found. Genotypes generated by transposon mutagenesis appeared in the screen at a frequency of ∼3%. Mutations affecting central metabolism and regulatory
- PMID 26468041
Japanese Journal
- Analysis of genes involved in exopolysaccharide production of Rothia mucilaginosa clinically isolated from a persistent apical periodontitis lesion
- Fujihira Tomohiro,Yamane Kazuyoshi,Yoshida Masahiro,Nambu Takayuki,Hayashi Hiroyuki
- Journal of Osaka Dental University 46(1), 157-164, 2012-04-00
- … mucilaginosa, we isolated three meshwork-like structures defective mutants (strains FA1, FA7 and FA8) by random transposon mutagenesis using the Epicentre EZ-TN5 transposome system. … The transposon insertion region of strain FA1 was determined by PCR direct sequencing analysis. …
- NAID 110009436547
- Isolation of a biofilm-defective mutant of Actinomyces from a patient with gingivitis
- Ogiso Kazuki,Nambu Takayuki,Kotsu Yoshio,Fukushima Hisanori,Ueda Masatoshi
- Journal of Osaka Dental University 46(1), 91-99, 2012-04-00
- … Using a random mutagenesis and biofilm assay for A. … The transposon in AOM0010 was inserted into the ANA2550 encoding 2-C-methyl-D-erythritol 4-phosphate (MEP) cytidylyltransferase, suggesting that the function of this gene is required for cell growth in A. … Unfortunately, this study could not enhance our understanding of Actinomyces biofilm formation, whereas the strategy of transposon mutagenesis was applicable to A. …
- NAID 110009436540
- Selection of Shewanella oneidensis MR-1 Gene-Knockout Mutants That Adapt to an Electrode-Respiring Condition
- TAJIMA Nozomi,KOUZUMA Atsushi,HASHIMOTO Kazuhito [他],WATANABE Kazuya
- Bioscience, biotechnology, and biochemistry 75(11), 2229-2233, 2011-11-23
- … MR-1 that adapted to an electrode-respiring condition were selected from a random transposon-insertion mutant library to obtain active current-generating mutants and identify relevant cellular components. … Transposon-insertion sites in the isolated mutants were identified by inverse PCR coupled to sequence analyses. … Southern blotting using a transposon probe was also performed to detect mutants that grew abundantly on the electrode. …
- NAID 10030397053
Related Links
- Transposon mutagenesis. Biparental matings of E. coli and the Synechococcus sp. were carried out as previously described (5). E. coli strain BW20767, which harbors the plasposon delivery vector pRL27 (15), was used as the donor ...
- Generation of doubly transgenic “seed males” for SB mutagenesis Once suitable transposon and transposase transgenic lines have been selected the next step is to breed mice together to obtain males that carry both transgenes.
★リンクテーブル★
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- 英
- transposon mutagenesis
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変異原性
- 関
- mutagenetic、mutagenic、mutagenicity、mutagenize