A large-scale zebrafish gene knockout resource for the genome-wide study of gene function.
Varshney GK, Lu J, Gildea DE, Huang H, Pei W, Yang Z, Huang SC, Schoenfeld D, Pho NH, Casero D, Hirase T, Mosbrook-Davis D, Zhang S, Jao LE, Zhang B, Woods IG, Zimmerman S, Schier AF, Wolfsberg TG, Pellegrini M, Burgess SM, Lin S.SourceDevelopmental Genomics Section, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892, USA;
Genome research.Genome Res.2013 Feb 26. [Epub ahead of print]
With the completion of the zebrafish genome sequencing project, it becomes possible to analyze the function of zebrafish genes in a systematic way. The first step in such an analysis is to inactivate each protein-coding gene by targeted or random mutation. Here we describe a streamlined pipeline usi
Mutagenesis and modeling to predict structural and functional characteristics of the Staphylococcus aureus MepA multidrug efflux pump.
Schindler BD, Patel D, Seo SM, Kaatz GW.SourceJohn D. Dingell Department of Veterans Affairs Medical Center, Detroit, Michigan, USA.
Journal of bacteriology.J Bacteriol.2013 Feb;195(3):523-33. doi: 10.1128/JB.01679-12. Epub 2012 Nov 21.
MepA is a multidrug and toxin extrusion (MATE) family protein and the only MATE protein encoded within the Staphylococcus aureus genome. Structural data for MATE proteins are limited to a single high-resolution example, NorM of Vibrio cholerae. Substitution mutations were created in MepA using gradi
Pathways of genetic adaptation: multistep origin of mutants under selection without induced mutagenesis in Salmonella enterica.
Quiñones-Soto S, Reams AB, Roth JR.SourceDepartment of Microbiology, University of California, Davis, California 95616, USA.
Genetics.Genetics.2012 Nov;192(3):987-99. doi: 10.1534/genetics.112.142158. Epub 2012 Aug 10.
In several bacterial systems, mutant cell populations plated on growth-restricting medium give rise to revertant colonies that accumulate over several days. One model suggests that nongrowing parent cells mutagenize their own genome and thereby create beneficial mutations (stress-induced mutagenesis
… We will mutagenize the auxin inducible promoter :: GUS transgenic plants, and we will analyze the ts-emb mutants which show an abberant activity of GUS gene. …
mu·ta·gen·ize (my t -j n z, -j -n z ). tr.v. mu·ta·gen·ized, mu·ta·gen·iz·ing, mu·ta·gen· iz·es. To cause or induce mutation in (a cell or an organism). mutagenize, mutagenise [ˈmjuːtədʒəˌnaiz]. vb (tr). (Life Sciences & Allied Applications ...
Definition of mutagenizing in the Medical Dictionary. mutagenizing explanation. Information about mutagenizing in Free online English dictionary. What is mutagenizing? Meaning of mutagenizing medical term. What does mutagenizing mean?