WordNet

a base found in DNA (but not in RNA) and derived from pyrimidine; pairs with adenine (同)T
a phosphoric ester of a nucleoside; the basic structural unit of nucleic acids (DNA or RNA) (同)base

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(胸腺のDNAからとる)白色結晶ピリミジン　チミン

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1. 母体血中の無細胞核酸を使った非侵襲的出生前検査 noninvasive prenatal testing using cell free nucleic acids in maternal blood
2. 遺伝性疾患の基本原則 basic principles of genetic disease
3. スタージウェーバー症候群 sturge weber syndrome
4. 甲状腺刺激ホルモンおよび甲状腺刺激ホルモン放出ホルモンに対する抵抗性 resistance to thyrotropin and thyrotropin releasing hormone
5. 先端巨大症の原因および臨床症状 causes and clinical manifestations of acromegaly

English Journal

Coupled-cluster and density functional theory studies of the electronic 0-0 transitions of the DNA bases.

Ovchinnikov VA1, Sundholm D.Author information 1University of Helsinki, P.O. Box 55 (A.I. Virtanens plats 1), 00014 Helsinki, Finland. vasily@chem.helsinki.fi Dage.Sundholm@helsinki.fi.AbstractThe 0-0 transitions of the electronic excitation spectra of the lowest tautomers of the four nucleotide (DNA) bases have been studied using linear-response approximate coupled-cluster singles and doubles (CC2) calculations. Excitation energies have also been calculated at the linear-response time-dependent density functional theory (TDDFT) level using the B3LYP functional. Large basis sets have been employed for ensuring that the obtained excitation energies are close to the basis-set limit. Zero-point vibrational energy corrections have been calculated at the B3LYP and CC2 levels for the ground and excited states rendering direct comparisons with high-precision spectroscopy measurements feasible. The obtained excitation energies for the 0-0 transitions of the first excited states of guanine tautomers are in good agreement with experimental values confirming the experimental assignment of the energetic order of the tautomers of the DNA bases. For the experimentally detected guanine tautomers, the first excited state corresponds to a π → π* transition, whereas for the tautomers of adenine, thymine, and the lowest tautomer of cytosine the transition to the first excited state has n → π* character. The calculations suggest that the 0-0 transitions of adenine, thymine, and cytosine are not observed in the absorption spectrum due to the weak oscillator strength of the formally symmetry-forbidden transitions, while 0-0 transitions of thymine have been detected in fluorescence excitation spectra.
Physical chemistry chemical physics : PCCP.Phys Chem Chem Phys.2014 Mar 5. [Epub ahead of print]
The 0-0 transitions of the electronic excitation spectra of the lowest tautomers of the four nucleotide (DNA) bases have been studied using linear-response approximate coupled-cluster singles and doubles (CC2) calculations. Excitation energies have also been calculated at the linear-response time-de
PMID 24595333

FeON-FeOFF: the Helicobacter pylori Fur regulator commutates iron-responsive transcription by discriminative readout of opposed DNA grooves.

Agriesti F1, Roncarati D, Musiani F, Del Campo C, Iurlaro M, Sparla F, Ciurli S, Danielli A, Scarlato V.Author information 1Department of Pharmacy and Biotechnology (FaBiT), University of Bologna, 40126 Bologna, Italy.AbstractMost transcriptional regulators bind nucleotide motifs in the major groove, although some are able to recognize molecular determinants conferred by the minor groove of DNA. Here we report a transcriptional commutator switch that exploits the alternative readout of grooves to mediate opposite output regulation for the same input signal. This mechanism accounts for the ability of the Helicobacter pylori Fur regulator to repress the expression of both iron-inducible and iron-repressible genes. When iron is scarce, Fur binds to DNA as a dimer, through the readout of thymine pairs in the major groove, repressing iron-inducible transcription (FeON). Conversely, on iron-repressible elements the metal ion acts as corepressor, inducing Fur multimerization with consequent minor groove readout of AT-rich inverted repeats (FeOFF). Our results provide first evidence for a novel regulatory paradigm, in which the discriminative readout of DNA grooves enables to toggle between the repression of genes in a mutually exclusive manner.
Nucleic acids research.Nucleic Acids Res.2014 Mar 1;42(5):3138-51. doi: 10.1093/nar/gkt1258. Epub 2013 Dec 9.
Most transcriptional regulators bind nucleotide motifs in the major groove, although some are able to recognize molecular determinants conferred by the minor groove of DNA. Here we report a transcriptional commutator switch that exploits the alternative readout of grooves to mediate opposite output
PMID 24322295

A sensitive fluorescence anisotropy method for detection of lead (II) ion by a G-quadruplex-inducible DNA aptamer.

Zhang D1, Yin L2, Meng Z2, Yu A3, Guo L1, Wang H4.Author information 1State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, PR China.2School of Chemical Engineering and Environment, Beijing Institute of Technology, Beijing, 100081, PR China.3Department of Chemistry, Renmin University of China, Beijing, 100872, PR China.4State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, PR China. Electronic address: hlwang@rcees.ac.cn.AbstractSensitive and selective detection of Pb(2+) is of great importance to both human health and environmental protection. Here we propose a novel fluorescence anisotropy (FA) approach for sensing Pb(2+) in homogeneous solution by a G-rich thrombin binding aptamer (TBA). The TBA labeled with 6-carboxytetramethylrhodamine (TMR) at the seventh thymine nucleotide was used as a fluorescent probe for signaling Pb(2+). It was found that the aptamer probe had a high FA in the absence of Pb(2+). This is because the rotation of TMR is restricted by intramolecular interaction with the adjacent guanine bases, which results in photoinduced electron transfer (PET). When the aptamer probe binds to Pb(2+) to form G-quadruplex, the intramolecular interaction should be eliminated, resulting in faster rotation of the fluorophore TMR in solution. Therefore, FA of aptamer probe is expected to decrease significantly upon binding to Pb(2+). Indeed, we observed a decrease in FA of aptamer probe upon Pb(2+) binding. Circular dichroism, fluorescence spectra, and fluorescence lifetime measurement were used to verify the reliability and reasonability of the sensing mechanism. By monitoring the FA change of the aptamer probe, we were able to real-time detect binding between the TBA probe and Pb(2+). Moreover, the aptamer probe was exploited as a recognition element for quantification of Pb(2+) in homogeneous solution. The change in FA showed a linear response to Pb(2+) from 10nM to 2.0μM, with 1.0nM limit of detection. In addition, this sensing system exhibited good selectivity for Pb(2+) over other metal ions. The method is simple, quick and inherits the advantages of aptamer and FA.
Analytica chimica acta.Anal Chim Acta.2014 Feb 17;812:161-7. doi: 10.1016/j.aca.2013.12.029. Epub 2014 Jan 3.
Sensitive and selective detection of Pb(2+) is of great importance to both human health and environmental protection. Here we propose a novel fluorescence anisotropy (FA) approach for sensing Pb(2+) in homogeneous solution by a G-rich thrombin binding aptamer (TBA). The TBA labeled with 6-carboxytet
PMID 24491777

Japanese Journal

PA52 DNA添加による液晶場の分子配向構造変化(物理・物性,ポスター発表,2013年日本液晶学会討論会)

吉田将之,菅野光,岩端一樹,坂口謙吾,古江広和
日本液晶学会討論会講演予稿集 (2013), "PA52-1"-"PA52-2", 2013-09-08
… adenine, thymine, guanine, and cytosine constitute a DNA molecule. … If the characteristics of LC doped with DNA depend on the kind of base, the nucleotide sequence analysis can be performed by utilizing LC. …
NAID 110009899253

塩基配列選択的な金属化によるナノギャップ構造形成に向けた鋳型DNAの作製

三友秀之,渡辺雪江,松尾保孝 [他],新倉謙一,居城邦治
高分子論文集 70(7), 337-340, 2013-07-25
… We have already reported the fabrication of sequence-selective platinum nano-wires using poly(guanine) and poly(adenine-thymine) diblock DNA, which is enzymatically polymerized as a template. … In this study, we prepared triblock DNA sequences using guanine as a platinum-binding natural nucleotide and 7-deaza-guanine as a platinum-nonbinding unnatural nucleotide for the construction of platinum nano-wires with nano-gap structures. …
NAID 10031188190

Hypoxanthine-guanine phosphoribosyltransferase部分欠損症の1例:―高度関節破壊を認めた症例―

王興栄,並木脩,豊島洋一,稲垣克記,山田裕一
昭和学士会雑誌 73(1), 43-47, 2013
症例は45歳男性，15歳より繰り返す痛風発作と両手関節痛を認め，両足関節痛と可動域制限を主訴に当院を受診となった．患者は既往歴に精神疾患と他院での急性腎不全による入院治療歴があった．これらの経緯より，われわれはプリン核酸代謝異常を有する先天的疾患に罹患している可能性を推測した．Hypoxanthine-guanine phosphoribosyltransferase（HPRT）遺伝子（HPRT1 …
NAID 130005056844