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a colorless crystalline amino acid found in protein; occurs in the hydrolysates of certain proteins; an essential component of human nutrition

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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2012/11/16 15:11:20」(JST)

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In enzymology, a threonine ammonia-lyase (EC 4.3.1.19) is an enzyme that catalyzes the chemical reaction

L-threonine 2-oxobutanoate + NH₃

Hence, this enzyme has one substrate, L-threonine, and two products, Alpha-Ketobutyric acid and NH₃.

This enzyme belongs to the family of lyases, specifically ammonia lyases, which cleave carbon-nitrogen bonds. The systematic name of this enzyme class is L-threonine ammonia-lyase (2-oxobutanoate-forming). Other names in common use include threonine deaminase, L-serine dehydratase, serine deaminase, L-threonine dehydratase, threonine dehydrase, L-threonine deaminase, threonine dehydratase, L-threonine hydro-lyase (deaminating), and L-threonine ammonia-lyase. This enzyme participates in glycine, serine and threonine metabolism and valine, leucine and isoleucine biosynthesis. It employs one cofactor, pyridoxal phosphate.

Enzyme regulation

This protein may use the morpheein model of allosteric regulation. ^[1]

Structural studies

As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes 1VE5, 2GN0, 2GN1, and 2GN2.

References

^ T. Selwood and E. K. Jaffe. (2011). "Dynamic dissociating homo-oligomers and the control of protein function.". Arch. Biochem. Biophys. 519 (2): 131–43. doi:10.1016/j.abb.2011.11.020. PMID 22182754. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=22182754.

Cohn MS, Phillips AT (1974). "Purification and characterization of a B6-independent threonine dehydratase from Pseudomonas putida". Biochemistry. 13 (6): 1208–14. doi:10.1021/bi00703a604. PMID 4814721.
NISHIMURA JS, GREENBERG DM (1961). "Purification and properties of L-threonine dehydrase of sheep liver". J. Biol. Chem. 236: 2684–91. PMID 14479973.
Phillips AT, Wood WA (1965). "The mechanism of action of 5'-adenylic acid-activated threonine dehydrase". J. Biol. Chem. 240 (12): 4703–9. PMID 5321308.
Shizuta Y, Nakazawa A, Tokushige M, Hayaishi O (1969). "Studies on the interaction between regulatory enzymes and effectors 3. Crystallization and characterization of adenosine 5'-monophosphate-dependent threonine deaminase from Escherichia coli". J. Biol. Chem. 244 (7): 1883–9. PMID 4889010.

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English Journal

13 C metabolic flux profiling of Pichia pastoris grown in aerobic batch cultures on glucose revealed high relative anabolic use of TCA cycle and limited incorporation of provided precursors of branched-chain amino acids.

Zhang M1, Yu XW1, Xu Y1, Jouhten P2, Swapna GVT3, Glaser RW4, Hunt JF5, Montelione GT3,6, Maaheimo H7, Szyperski T8.
The FEBS journal.FEBS J.2017 Sep;284(18):3100-3113. doi: 10.1111/febs.14180. Epub 2017 Aug 7.
PMID 28731268

Biochemical and functional characterization of MRA_1571 of Mycobacterium tuberculosis H37Ra and effect of its down-regulation on survival in macrophages.

Sharma R1, Keshari D1, Singh KS1, Singh SK2.
Biochemical and biophysical research communications.Biochem Biophys Res Commun.2017 Jun 10;487(4):892-897. doi: 10.1016/j.bbrc.2017.04.149. Epub 2017 May 2.
PMID 28465237

Isolation and amino acid sequence of a dehydratase acting on d-erythro-3-hydroxyaspartate from Pseudomonas sp. N99, and its application in the production of optically active 3-hydroxyaspartate.

Nagano H1, Shibano K1, Matsumoto Y1, Yokota A1, Wada M1.
Bioscience, biotechnology, and biochemistry.Biosci Biotechnol Biochem.2017 Jun;81(6):1156-1164. doi: 10.1080/09168451.2017.1295804. Epub 2017 Mar 14.
PMID 28290777

Japanese Journal

ビタミンB<SUB>6</SUB>欠乏食給餌ラットにおける分枝鎖アミノ酸添加の影響

開元多恵,木村元秋,細川紘美 [他],渋谷まゆみ,前田英雄
日本栄養・食糧学会誌 65(2), 65-73, 2012
ビタミンB<SUB>6</SUB> (B6) 欠乏食給餌下, 餌に4.75% (w/w) の分枝鎖アミノ酸 (BCAA) を添加した時にラットの生体内に与える影響について検討した。餌にB6を含有したBCAA無添加食群 (C群) と添加食群 (aaC群), B6を含有しないBCAA無添加食群 (D群) と添加食群 (aaD群) の4種類の食餌で5週齢のウィスター系雄ラット各6 …
NAID 130002129279

Gene Cloning and Expression of Pyridoxal 5'-Phosphate-Dependent L-threo-3-Hydroxyaspartate Dehydratase from Pseudomonas sp. T62, and Characterization of the Recombinant Enzyme

Murakami Tomoko,Maeda Takayuki,Yokota Atsushi [他],WADA Masaru
The journal of biochemistry 145(5), 661-668, 2009-05-01
… L-threo-3-Hydroxyaspartate dehydratase (L-THA DH, EC 4.3.1.16), which catalyses the cleavage of L-threo-3-hydroxyaspartate (L-THA) to oxalacetate and ammonia, has been purified from the soil bacterium Pseudomonas sp. …
NAID 10025589107

Effect of L-Serine on the Biosynthesis of Aromatic Amino Acids in Escherichia coli

Tazuya-Murayama Keiko,Aramaki Hironori,Mishima Motohiro [他],SAITO Keiko,ISHIDA Shiho,YAMADA Kazuko
Journal of nutritional science and vitaminology 52(4), 256-260, 2006-08
… It has been reported that this L-serine inhibition may be prevented by the addition of L-isoleucine or L-threonine to the medium. … The specific activity of prephenate dehydratase decreased in cells grown in the presence of L-serine. … However, L-serine did not inhibit in vitro prephenate dehydratase activity, and the expression of pheA, which encodes the prephenate dehydratase, was not depressed by L-serine. …
NAID 110004789053

「スレオニン脱水酵素」

L-threonine ammonia-lyase
Identifiers
EC number	4.3.1.19
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / EGO
Search
PMC	articles
PubMed	articles
NCBI Protein	search

　　[★]

英: threonine dehydratase
関: スレオニンデヒドラターゼ、トレオニンデヒドラターゼ

「スレオニンデヒドラターゼ」

　　[★]

英: threonine dehydratase
関: スレオニン脱水酵素、トレオニンデヒドラターゼ

「トレオニンデヒドラターゼ」

　　[★]

英: threonine dehydratase
関: スレオニン脱水酵素、スレオニンデヒドラターゼ

「dehydratase」

　　[★]

n.

デヒドラターゼ、脱水酵素

関: anhydrase、dehydrase、hydrase、hydro-lyase

「threonine」

　　[★] スレオニン L-threonine T Thr 　

[pmid22182754-1] T. Selwood and E. K. Jaffe. (2011). "Dynamic dissociating homo-oligomers and the control of protein function.". Arch. Biochem. Biophys. 519 (2): 131–43. doi:10.1016/j.abb.2011.11.020. PMID 22182754. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=22182754.

リンク元	「スレオニン脱水酵素」「スレオニンデヒドラターゼ」「トレオニンデヒドラターゼ」
関連記事	「dehydratase」「threonine」

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案内

案内

threonine dehydratase