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- (ある地方・時代の)植物群(相),全植物
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- 1. 嫌気菌：ヒトの正常細菌叢における歴史および役割 anaerobic bacteria history and role in normal human flora
- 2. 嫌気性細菌感染 anaerobic bacterial infections
- 3. 羊水感染症（絨毛膜羊膜炎） intraamniotic infection chorioamnionitis
- 4. 女性における再発性尿路感染症 recurrent urinary tract infection in women
- 5. 細菌性膣炎 bacterial vaginosis
English Journal
- An activator for pyruvoyl-dependent l-aspartate α-decarboxylase is conserved in a small group of the γ-proteobacteria including Escherichia coli.
- Nozaki S1, Webb ME, Niki H.Author information 1Microbial Genetics Laboratory, Genetic Strains Research Center, National Institute of Genetics 1111 Yata, Mishima, Shizuoka, 411-8540, Japan.AbstractIn bacteria, β-alanine is formed via the action of l-aspartate α-decarboxylase (PanD) which is one of the small class of pyruvoyl-dependent enzymes. The pyruvoyl cofactor in these enzymes is formed via the intramolecular rearrangement of a serine residue in the peptide backbone leading to chain cleavage and formation of the covalently-bound cofactor from the serine residue. This reaction was previously thought to be uncatalysed. Here we show that in Escherichia coli, PanD is activated by the putative acetyltransferase YhhK, subsequently termed PanZ. Activation of PanD both in vivo and in vitro is PanZ-dependent. PanZ binds to PanD, and we demonstrate that a PanZ(N45A) site-directed mutant is unable to enhance cleavage of the proenzyme PanD despite retaining affinity for PanD. This suggests that the putative acetyltransferases domain of PanZ may be responsible for activation to enhance the processing of PanD. Although panD is conserved among most bacteria, the panZ gene is conserved only in E. coli-related enterobacterial species including Shigella, Salmonella, Klebsiella and Yersinia. These bacteria are found predominantly in the gut flora where pantothenate is abundant and regulation of PanD by PanZ allows these organisms to closely regulate production of β-alanine and hence pantothenate in response to metabolic demand.
- MicrobiologyOpen.Microbiologyopen.2012 Sep;1(3):298-310. doi: 10.1002/mbo3.34. Epub 2012 Aug 14.
- In bacteria, β-alanine is formed via the action of l-aspartate α-decarboxylase (PanD) which is one of the small class of pyruvoyl-dependent enzymes. The pyruvoyl cofactor in these enzymes is formed via the intramolecular rearrangement of a serine residue in the peptide backbone leading to chain cl
- PMID 23170229
- Variation in extragenic repetitive DNA sequences in Pseudomonas syringae and potential use of modified REP primers in the identification of closely related isolates.
- Cepni E1, Gürel F.Author information 1Department of Molecular Biology and Genetics, Faculty of Sciences, Istanbul University, Istanbul, Turkey.AbstractIn this study, Pseudomonas syringe pathovars isolated from olive, tomato and bean were identified by species-specific PCR and their genetic diversity was assessed by repetitive extragenic palindromic (REP)-PCR. Reverse universal primers for REP-PCR were designed by using the bases of A, T, G or C at the positions of 1, 4 and 11 to identify additional polymorphism in the banding patterns. Binding of the primers to different annealing sites in the genome revealed additional fingerprint patterns in eight isolates of P. savastanoi pv. savastanoi and two isolates of P. syringae pv. tomato. The use of four different bases in the primer sequences did not affect the PCR reproducibility and was very efficient in revealing intra-pathovar diversity, particularly in P. savastanoi pv. savastanoi. At the pathovar level, the primer BOX1AR yielded shared fragments, in addition to five bands that discriminated among the pathovars P. syringae pv. phaseolicola, P. savastanoi pv. savastanoi and P. syringae pv. tomato. REP-PCR with a modified primer containing C produced identical bands among the isolates in a pathovar but separated three pathovars more distinctly than four other primers. Although REP- and BOX-PCRs have been successfully used in the molecular identification of Pseudomonas isolates from Turkish flora, a PCR based on inter-enterobacterial repetitive intergenic concensus (ERIC) sequences failed to produce clear banding patterns in this study.
- Genetics and molecular biology.Genet Mol Biol.2012 Jul;35(3):650-6. doi: 10.1590/S1415-47572012005000040. Epub 2012 Jul 5.
- In this study, Pseudomonas syringe pathovars isolated from olive, tomato and bean were identified by species-specific PCR and their genetic diversity was assessed by repetitive extragenic palindromic (REP)-PCR. Reverse universal primers for REP-PCR were designed by using the bases of A, T, G or C at
- PMID 23055805
- Effects of immune stress on growth performance, immunity, and cecal microflora in chickens.
- Yang XJ1, Li WL, Feng Y, Yao JH.Author information 1Northwest A&F University, Yangling, Shaanxi, China.AbstractImmune stress is the loss of immune homeostasis by external forces. This study investigated the effects of different types of immune stress on growth performance, immunity, and the distribution of cecal microflora in broiler chickens. In total, 540 one-day-old Cobb 500 broilers were randomly assigned to receive 1 of 5 (n = 108 birds/group) treatments: 1) no vaccination; 2) simplified vaccination, which included the infectious bronchitis vaccine (H120), the inactivated avian influenza vaccine (AI), the live vaccine strain Clone-30 of the Newcastle disease virus (NDV), and the combined inactive vaccine for infectious bursal diseases and the Newcastle disease vaccine (ND-IB); 3) normal vaccination (simplified vaccination + second dose of ND-IB, H120, and AI); 4) lipopolysaccharide (LPS) stress (normal vaccination+LPS); or 5) cyclophosphamide (CPM) stress (normal vaccination+CPM).The results showed that the average BW and average feed intake decreased significantly after treatment with LPS or CPM (P < 0.05). Chickens that were challenged by LPS or CPM had a lower ileal CP digestibility than that of the control group (P < 0.01). Compared with the control group, the levels of secreted IgA decreased significantly at 42 d of age in the chickens that were treated with LPS or CPM (P < 0.01). The proliferation of the peripheral blood mononuclear cell and the levels of serum IgG in the LPS-challenged chickens were higher than those in the control group chickens at 21 and 42 d of age, respectively (P < 0.05). Six clusters were identified at 21 d of age, but cluster 6 was a single sample. Only 5 clusters were identified at 42 d of age. The enterobacterial repetitive intergenic consensus (ERIC)-PCR fingerprints of the cecal samples from the no vaccination and the simplified vaccination groups clustered together with high coefficients. The ERIC-PCR fingerprints of the 3 cecal samples from the CPM and LPS treatment groups clustered together with high coefficients among them. The ERIC-PCR fingerprints of the microbial flora of the cecal contents revealed the potential effects of immune stress on the microbial populations of treated birds. These data suggest that broilers with simplified vaccinations or without vaccinations can achieve the same growth performance as broilers with general vaccinations, but immune stress can break the homeostasis of cecal microflora and impair intestinal mucosal immune function.
- Poultry science.Poult Sci.2011 Dec;90(12):2740-6. doi: 10.3382/ps.2011-01591.
- Immune stress is the loss of immune homeostasis by external forces. This study investigated the effects of different types of immune stress on growth performance, immunity, and the distribution of cecal microflora in broiler chickens. In total, 540 one-day-old Cobb 500 broilers were randomly assigne
- PMID 22080012
Japanese Journal
- 市場動向 乳酸菌と腸内環境改善素材--免疫調整機能など進む多角的な提案
- 食品と開発 45(4), 43-51, 2010-04
- NAID 40017067823
- Effect of Nitric Oxide on β-Glucan/Indomethacin-Induced Septic Shock(Biopharmacy)
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- Biological & pharmaceutical bulletin 28(7), 1254-1258, 2005-07-01
- … The lethality would be strongly related to the translocation of enterobacterial flora to the peritoneal cavity and disruption of the cytokine network. … These results suggest a significant loss of the bactericidal activity of macrophages on the administration of a NOS inhibitor which enhanced the rate of enterobacterial invasion to the peritoneal cavity, resulting in systemic inflammatory response syndrome. …
- NAID 10016665495
- 新ガラクトオリゴ糖含有液糖がヒト腸内菌叢に及ぼす影響
- 松本 一政,高田 敏彦,結城 功勝,川上 幸治,酒井 隆史,野本 康二,木村 一雅,松本 圭介,飯野 久和
- 腸内細菌学雑誌 = Journal of intestinal microbiology 18(1), 25-35, 2004-01-01
- 4'-galactosyllactose (4'-GL) を含む2～4糖を主成分とする新規な組成のガラクトオリゴ糖 (新GOS) 液糖のプレバイオティクス作用を調べるため, 1) 22菌属62菌株の腸内細菌構成菌株による新GOS構成オリゴ糖構成分子のin vitro における資化性, および2) ヒト飲用試験における腸内菌叢への影響を解析した. in vitro 資化性試験においては, 新GOS2 …
- NAID 10027792477
Related Links
- Moreover, we have hypothesized that an additional role of the GI regulatory system is to control the condition of enterobacterial flora consisting of at least 1,000 species and >100 trillion bacteria. Recently, role of the enterobacterial ...
- Analysis of enterobacterial flora by RFLP analysis method Comments It has been possible to extract DNAs efficiently from hard cell-wall gram-positive bacteria predominantly present in intestine. While the reagents cost remained ...
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★リンクテーブル★
| リンク元 | 「intestinal bacterial flora」 |
| 関連記事 | 「enterobacterial」「enterobacteria」「flora」 |
「intestinal bacterial flora」
「enterobacterial」
- adj.
- 腸内細菌の、腸内細菌性の
「enterobacteria」
「flora」