Choline-phosphate cytidylyltransferase (EC 2.7.7.15) is an enzyme that catalyzes the chemical reaction
CTP + choline phosphate diphosphate + CDP-choline
where the two substrates of this enzyme are CTP and choline phosphate, and the two products are diphosphate and CDP-choline. It is responsible for regulating phosphatidylcholine content in membranes.
This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing nucleotide groups (nucleotidyltransferases). The systematic name of this enzyme class is CTP:choline-phosphate cytidylyltransferase. Other names in common use include phosphorylcholine transferase, CDP-choline pyrophosphorylase, CDP-choline synthetase, choline phosphate cytidylyltransferase, CTP-phosphocholine cytidylyltransferase, CTP:phosphorylcholine cytidylyltransferase, cytidine diphosphocholine pyrophosphorylase, phosphocholine cytidylyltransferase, phosphorylcholine cytidylyltransferase, and phosphorylcholine:CTP cytidylyltransferase. This enzyme participates in aminophosphonate metabolism and glycerophospholipid metabolism.
Structural studies
As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 1PEH and 1PEI.
References
BORKENHAGEN LF, KENNEDY EP (1957). "The enzymatic synthesis of cytidine diphosphate choline". J. Biol. Chem. 227 (2): 951–62. PMID 13463016.
KENNEDY EP, WEISS SB (1956). "The function of cytidine coenzymes in the biosynthesis of phospholipides". J. Biol. Chem. 222 (1): 193–214. PMID 13366993.
Williams-Ashman HG, Banks J (1956). "Participation of cytidine coenzymes in the metabolism of choline by seminal vesicles". J. Biol. Chem. 223 (1): 509–521. PMID 13376620.
v
t
e
Metabolism: lipid metabolism
phospholipids
Anabolism
to diacylglycerol: Acyltransferase
Phosphatidate phosphatase
2C
Choline kinase
CHKA
CHKB
Choline-phosphate cytidylyltransferase
phosphatidylinositol glycan anchor biosynthesis:
PIGA
PIGB
PIGC
PIGF
PIGG
PIGH
PIGK
PIGL
PIGM
PIGN
PIGO
PIGP
PIGQ
PIGS
PIGT
PIGU
PIGV
PIGW
PIGX
PIGY
PIGZ
cardiolipin: Tafazzin
Catabolism
Phospholipase C
Diacylglycerol kinase
DGKA
DGKB
DGKD
DGKE
DGKG
DGKH
DGKI
DGKK
DGKQ
DGKZ
Phosphoric monoester hydrolases
Inositol-phosphate phosphatase
v
t
e
Transferases: phosphorus-containing groups (EC 2.7)
2.7.1-2.7.4: phosphotransferase/kinase (PO4)
2.7.1: OH acceptor
Hexo-
Gluco-
Fructo-
Hepatic
Galacto-
Phosphofructo-
1
Liver
Muscle
Platelet
2
Riboflavin
Shikimate
Thymidine
ADP-thymidine
NAD+
Glycerol
Pantothenate
Mevalonate
Pyruvate
Deoxycytidine
PFP
Diacylglycerol
Phosphoinositide 3
Class I PI 3
Class II PI 3
Sphingosine
Glucose-1,6-bisphosphate synthase
2.7.2: COOH acceptor
Phosphoglycerate
Aspartate kinase
2.7.3: N acceptor
Creatine
2.7.4: PO4 acceptor
Phosphomevalonate
Adenylate
Nucleoside-diphosphate
Uridylate
Guanylate
Thiamine-diphosphate
2.7.6: diphosphotransferase (P2O7)
Ribose-phosphate diphosphokinase
Thiamine diphosphokinase
2.7.7: nucleotidyltransferase (PO4-nucleoside)
Polymerase
DNA polymerase
DNA-directed DNA polymerase
I
II
III
IV
V
RNA-directed DNA polymerase
Reverse transcriptase
Telomerase
DNA nucleotidylexotransferase/Terminal deoxynucleotidyl transferase
5. 乳房のMRIおよび新しい技術 mri of the breast and emerging technologies
English Journal
Efficient multi-enzyme-catalyzed CDP-choline production driven by an ATP donor module.
Liu Y1,2,3, Wang J1,2, Xu C4, Chen Y1,2,3, Yang J4, Liu D1,2, Niu H1,2, Jiang Y4, Yang S5,6, Ying H1,2,3.
Applied microbiology and biotechnology.Appl Microbiol Biotechnol.2017 Feb;101(4):1409-1417. doi: 10.1007/s00253-016-7874-0. Epub 2016 Oct 13.
Cytidine diphosphate choline (CDP-choline) has been applied for treating acute craniocerebral injury and allowing recovery of consciousness after brain surgery. In this study, an acetate kinase (ACK)/acetyl phosphate system was used to supply ATP and combined with Escherichia coli-overexpressed CMP
The cytidylyltransferases are a family of enzymes that utilize cytidine 5'-triphosphate (CTP) to synthesize molecules that are typically precursors to membrane phospholipids. The most extensively studied cytidylyltransferase is CTP:phosphocholine cytidylyltransferase (CCT), which catalyzes conversio
Membrane lipid compositional sensing by the inducible amphipathic helix of CCT.
Cornell RB1.
Biochimica et biophysica acta.Biochim Biophys Acta.2016 Aug;1861(8 Pt B):847-61. doi: 10.1016/j.bbalip.2015.12.022. Epub 2015 Dec 31.
The amphipathic helical (AH) membrane binding motif is recognized as a major device for lipid compositional sensing. We explore the function and mechanism of sensing by the lipid biosynthetic enzyme, CTP:phosphocholine cytidylyltransferase (CCT). As the regulatory enzyme in phosphatidylcholine (PC)
… We developed new methods for the microdetermination of lung phospholipids and choline-containing metabolites in rat alveolar type II cells using high-performance liquid chromatography (HPLC). … This analytical study was performed in labeling experiments with [?H]choline to get insight into the rate-limiting step of phosphatidylcholine(PC) de novo synthesis in the cells. … The choline containing metabolites, i. …
Proteins known to be involved in the 2 steps of the subpathway in this organism are: Choline-phosphate cytidylyltransferase A (), Choline-phosphate cytidylyltransferase B (PCYT1B) Cholinephosphotransferase 1 (), Choline ...
Controls phosphatidylcholine synthesis. ... Proteins known to be involved in the 2 steps of the subpathway in this organism are: Choline-phosphate cytidylyltransferase A (PCYT1A), Choline-phosphate cytidylyltransferase B () ...
![Choline-Phosphate Cytidylyltransferase by Willy, Nethanel [Paperback] | Jet.com](https://1mg.info/0/j/2/0083.jpg)
