抗毒素血清
WordNet
- an amber, watery fluid, rich in proteins, that separates out when blood coagulates (同)blood_serum
PrepTutorEJDIC
- リンパ液 / 血清
- serial / series / sermon
UpToDate Contents
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English Journal
- Study on camel IgG purification: A new approach to prepare Naja Naja Oxiana antivenom as passive immunization for therapy.
- Khamehchian S1, Zolfagharian H1, Mohammadpour Dounighi N1, Tebianian M1, Madani R2.Author information 1Department of Venomous Animals and Antivenom; Razi Vaccine and Serum Research Institute; Karaj, Iran.2Department of Biotechnology; Razi Vaccine and Serum Research Institute; Karaj, Iran.AbstractA combined process of ammonium sulfate precipitation (salting out) and ion-exchange chromatography on DEAE-Sepharose CL-6B was used to prepare camel antivenom (IgG) against Naja Naja Oxiana for therapy. In the ammonium sulfate precipitation, the best condition for fractionation of IgG from the other proteins in camel serum was 55% precipitate. The camel IgG presented as two bands with molecular masses of 250 and 100 kDa, the latter corresponding to heavy chain IgG, on 10% gel electrophoresis. A trace amount of non IgG proteins was not isolated and remained in this precipitate. Therefore in order to effectively separate albumin and the other nonspecific proteins from the IgG, the 25% precipitate of ammonium sulfate precipitation of serum was subjected to DEAE-Sepharose CL-6B column chromatography. A peak of antibody (IgG) could be obtained by elution with sodium phosphate buffer. In this stage, two bands of molecular masses of 150 and 75 kDa were observed on 7% gel electrophoresis. A comparative study was performed between camel IgG and conventional horse F(ab) 2 antivenoms in term of potency (serum neutralization test and ELISA). Our results showed that the potency of camel antivenom was 4-fold higher than that of horse. It is suggested the combined ammonium sulfate precipitation and ion-exchange chromatography process effectively removed residual proteins in the final camel IgG preparation and can be a suitable method for large-scale refinement of therapeutic camel antivenoms.
- Human vaccines & immunotherapeutics.Hum Vaccin Immunother.2014 Mar 18;10(6). [Epub ahead of print]
- A combined process of ammonium sulfate precipitation (salting out) and ion-exchange chromatography on DEAE-Sepharose CL-6B was used to prepare camel antivenom (IgG) against Naja Naja Oxiana for therapy. In the ammonium sulfate precipitation, the best condition for fractionation of IgG from the other
- PMID 24642472
- Rapid diagnosis of Naja atra snakebites.
- Hung DZ1, Lin JH, Mo JF, Huang CF, Liau MY.Author information 1Division of Toxicology, China Medical University Hospital , Taichung , Taiwan.AbstractAbstract Background. The clinical diagnosis of snakebites is critical and necessary in many parts of the world, especially in Southeastern Asia, where venomous snakebites are a burden on public health. It is difficult to define or recognize the species of venomous snake because of the overlapping clinical manifestations of envenomations. A quick and reliable method for identifying the snake species is necessary. We designed and tested a strip of lateral flow system for the diagnosis of cobra snake bites in Taiwan. Methods. We developed a kit based on an immunochromatographic method for rapid detection of cobra (Naja atra) venom in human serum. The test and control lines composed of 1 mg/ml polyclonal duck antivenom and 0.5 mg/ml goat anti-rabbit immunoglobulin antibody solutions, respectively, were coated on nitrocellulose strips. Colloidal gold was conjugated with rabbit polyclonal anti-cobra venom antibodies. From July 2007 to December 2012, we used the kit to test serum from snakebite patients and to examine the agreement between our rapid test and the currently used sandwich enzyme-linked immunosorbent assay (ELISA). Results. Our kit was able to detect cobra venom in serum samples in 20 minutes with a detection limit of 5 ng/ml. An absence of cross-reactivity with other non-cobra venoms from Taiwan was noted in vitro. A total of 88 snakebite patients (34 cobra and 54 other non-cobra) were tested. The sensitivity of the strips based on the ELISA results was 83.3% and the specificity was 100%. There was a strong agreement between the results of the ELISA and immunochromatographic strips (κ = 0.868). Discussion and conclusions. This data indicates that an immunochromatographic strip might be suitable for cobra venom detection and could be used as a quick diagnostic tool in cases of N. atra snakebite.
- Clinical toxicology (Philadelphia, Pa.).Clin Toxicol (Phila).2014 Mar;52(3):187-91. doi: 10.3109/15563650.2014.887725.
- Abstract Background. The clinical diagnosis of snakebites is critical and necessary in many parts of the world, especially in Southeastern Asia, where venomous snakebites are a burden on public health. It is difficult to define or recognize the species of venomous snake because of the overlapping cl
- PMID 24580058
- Immunological profile of antivenoms: Preclinical analysis of the efficacy of a polyspecific antivenom through antivenomics and neutralization assays.
- Gutiérrez JM1, Lomonte B2, Sanz L3, Calvete JJ4, Pla D5.Author information 1Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica. Electronic address: jose.gutierrez@ucr.ac.cr.2Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.3Instituto de Biomedicina de Valencia, CSIC, Spain.4Instituto de Biomedicina de Valencia, CSIC, Spain. Electronic address: jcalvete@csic.es.5Instituto de Biomedicina de Valencia, CSIC, Spain. Electronic address: dpla@ibv.csic.es.AbstractParenteral administration of animal-derived antivenoms constitutes the mainstay in the treatment of snakebite envenomings. Despite the fact that this therapy has been available for over a century, the detailed understanding of the neutralizing and immunoreactivity profiles of the majority of antivenoms is pending. Currently, a combination of preclinical neutralization tests and 'antivenomics', i.e. a proteomic-based assessment of antivenom immunoreactivity, provides a powerful analytical platform to investigate the preclinical efficacy of antivenoms. In this review, the studies performed on the polyvalent antivenom manufactured by Instituto Clodomiro Picado, Costa Rica, are summarized. This antivenom is prepared by immunizing horses with a mixture of the venoms of Bothrops asper, Crotalus simus and Lachesis stenophrys, and is used in Central America for the treatment of envenomings by viperid species. Overall, the antivenom shows a widespread pattern of immunological reactivity against homologous and heterologous venoms, which correlates with its ability to neutralize lethal, hemorrhagic, myotoxic, coagulant, defibrinogenating, phospholipase A2 and proteinase activities of viperid venoms. At the same time, antivenomics detected several venom components against which the antivenom shows only partial or negligible immunorecognition, such as low molecular mass vasoactive peptides, disintegrins, and some phospholipases A2, P-I metalloproteinases and serine proteinases. Such information can be used to design strategies for enhancing the antibody response of horses against poorly immunogenic, toxicologically-relevant venom components in order to further improve the efficacy of this antivenom.
- Journal of proteomics.J Proteomics.2014 Feb 28. pii: S1874-3919(14)00070-0. doi: 10.1016/j.jprot.2014.02.021. [Epub ahead of print]
- Parenteral administration of animal-derived antivenoms constitutes the mainstay in the treatment of snakebite envenomings. Despite the fact that this therapy has been available for over a century, the detailed understanding of the neutralizing and immunoreactivity profiles of the majority of antiven
- PMID 24583507
Japanese Journal
- 日本小児アレルギー学会誌 = The Japanese journal of pediatric allergy and clinical immunology 22(3), 357-362, 2008-08-01
- NAID 10026940449
- ハブ咬傷局所のEDTA-Ca塩治療効果に関する実験病理学的研究
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★リンクテーブル★
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- 英
- antitoxin serum, antivenom serum