Biotechnology and bioengineering.Biotechnol Bioeng.2016 Jan;113(1):173-81. doi: 10.1002/bit.25694. Epub 2015 Aug 19.
More than 80 years after its discovery, penicillin is still a widely used and commercially highly important antibiotic. Here, we analyse the metabolic network of penicillin synthesis in Penicillium chrysogenum based on the concept of elementary flux modes. In particular, we consider the synthesis of
Metabolic flux analysis of the halophilic archaeon Haladaptatus paucihalophilus.
Liu G1, Zhang M1, Mo T2, He L3, Zhang W1, Yu Y4, Zhang Q5, Ding W6.
Biochemical and biophysical research communications.Biochem Biophys Res Commun.2015 Nov 27;467(4):1058-62. doi: 10.1016/j.bbrc.2015.09.174. Epub 2015 Oct 9.
This work reports the (13)C-assisted metabolic flux analysis of Haladaptatus paucihalophilus, a halophilic archaeon possessing an intriguing osmoadaption mechanism. We showed that the carbon flow is through the oxidative tricarboxylic acid (TCA) cycle whereas the reductive TCA cycle is not operative
Probing the chemical mechanism of saccharopine reductase from Saccharomyces cerevisiae using site-directed mutagenesis.
Vashishtha AK1, West AH2, Cook PF3.
Archives of biochemistry and biophysics.Arch Biochem Biophys.2015 Oct 15;584:98-106. doi: 10.1016/j.abb.2015.08.023. Epub 2015 Sep 3.
Saccharopine reductase catalyzes the reductive amination of l-α-aminoadipate-δ-semialdehyde with l-glutamate to give saccharopine. Two mechanisms have been proposed for the reductase, one that makes use of enzyme side chains as acid-base catalytic groups, and a second, in which the reaction is cat