Trizol

トリゾール

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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2014/05/16 17:46:35」(JST)

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TRIzol reagent contains Guanidinium thiocyanate and Phenol.

TRIzol is a chemical solution used in RNA/DNA/protein extraction, by the reference paper from Piotr Chomczyński and Sacchi, N. in 1987.

TRIzol is the brand name of the product from Ambion part of Life Technologies, and the brand name from MRC, which was founded by Chomczynski, is TRI Reagent.

The correct name of the method is Guanidinium thiocyanate-phenol-chloroform extraction. The use of TRIzol can result in DNA and RNA yields comparable to other extraction methods. An alternative method for RNA extraction is phenol extraction and TCA/acetone precipitation. Chloroform should be exchanged with 1-bromo-3-chloropropane when using the new generation TRI Reagent.

TRIzol is light sensitive and is often stored in a dark-colored, glass container covered in foil. It must be kept below room temperature.

When used, it resembles cough syrup, bright pink. The smell of the phenol is extremely strong. TRIzol works by maintaining RNA integrity during tissue homogenization, while at the same time disrupting and breaking down cells and cell components.

Caution should be taken while using TRIzol (specifically phenol) and Chloroform.

Hazards

Exposure to TRIzol can be a serious health hazard. Exposure can lead to serious chemical burns and permanent scarring. A lab coat, gloves and a plastic apron are recommended

References

External links

• RNA extraction using trizol protocol on OpenWetWare

English Journal

• MicroRNA-199a* regulates the expression of cyclooxygenase-2 in human chondrocytes.

• Akhtar N, Haqqi TM.SourceCorrespondence to Tariq M Haqqi, Department of Medicine/Rheumatology, Rammelkamp-327, MetroHealth Medical Center/Case Western Reserve University, 2500 Metro Health Drive, Cleveland, Ohio 44109, USA; thaqqi@metrohealth.org.
• Annals of the rheumatic diseases.Ann Rheum Dis.2012 Jun;71(6):1073-80. Epub 2012 Jan 31.
• OBJECTIVE: Cyclooxygenase-2 (COX-2) expression is associated with the pathogenesis of chronic inflammation and pain in osteoarthritis (OA). A study was undertaken to determine whether interleukin-1β (IL-1β)-mediated induction of COX-2 can be regulated by microRNAs (miRNAs) in OA.METHODS: Human cho
• PMID 22294637

• S262A mutation abolishes protective effects of connexin 43 against hypothermic preservation-induced injury in cardiomyocytes.

• Xu HJ, Chen WL, Wang Y, Zheng MZ, Chen YY, Shen YL.SourceDepartment of Physiology, Wenzhou Medical College, Wenzhou, China.
• The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation.J Heart Lung Transplant.2012 Jun;31(6):663-9.
• BACKGROUND: Alleviation of cold preservation-induced injury is a critical part of the heart transplantation process. In this study we investigate the protective effect of connexin 43 (Cx43) overexpression against hypothermic preservation-induced injury in cardiomyocytes.METHODS: Total RNA was prepar
• PMID 22608771

Japanese Journal

• ACCUMULATION OF INTER-α-TRYPSIN INHIBITOR CHAINS IN HUMAN BENIGN AND MALIGNANT LIPOMATOUS TUMORS.

• Tabata Izuru,Nishida Yoshihiro,Lisheng Zhuo,Yoneda Masahiko,Sugiura Hideshi,Nakashima Hiroatsu,Yamada Yoshihisa,Kimata Koji,Iwata Hisashi
• Connective tissue 34(1), 65, 2002-04
• … Total RNA was isolated with TRIzol, and subjected to RT-PCR analysis using the specific primers for bikunin, HC1, HC2 and HC3. …
• NAID 110004002493

Related Links

• TRIzol 試薬：インビトロジェン株式会社

インビトロジェンは研究者の方々にバイオ関連研究用試薬・ライフサイエンス研究用試薬 ・機器の輸入販売、受託サービス（生産・研究）をおこなっています。

• 核酸精製/TRIzol Plus RNA Purification Kit：インビトロジェン株式会社

TRIzol® Plus RNA Purification Kitを使えばマイクロアレイなどに適した純度のTotal RNAが得られます. 特長：. Q社R製品より高収率です。（図2）; トリゾルはRNA精製の スタンダードです。 PureLinkキットにトリゾル試薬を併用するとゲノムDNAのコンタミが 大幅 ...

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リンク元

「トリゾール」

「トリゾール」

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英

Trizol

• www.invitrogen.comの製品名。
• TRIzol®試薬は、トータルRNAを細胞および組織から分離するために最適なフェノールおよびグアニジンイソチオシアネートの、すぐに使用可能な単相溶液です。 サンプルのホモジナイズまたは溶解の間、TRIZOL®試薬は、細胞を破壊し、細胞成分を溶解しながらRNAの完全性を維持します。(参考1)

参考

• 1.

http://products.invitrogen.com/ivgn/product/15596026

• 2. RNA抽出の原理

http://lablogue.seesaa.net/article/143393682.html