ラルストニア、ルストニア属、Ralstonia属
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- 1. 新生児における酸素のモニタリングおよび治療 oxygen monitoring and therapy in the newborn
English Journal
- Dual-species biofilm formation by Escherichia coli O157:H7 and environmental bacteria isolated from fresh-cut processing facilities.
- Liu NT1, Nou X2, Lefcourt AM3, Shelton DR3, Lo YM4.Author information 1Environmental Microbial and Food Safety Laboratory, USDA Agricultural Research Service, Beltsville, MD 20705, United States; Department of Nutrition and Food Science, University of Maryland, College Park, MD 20740, United States.2Environmental Microbial and Food Safety Laboratory, USDA Agricultural Research Service, Beltsville, MD 20705, United States. Electronic address: xiangwu.nou@ars.usda.gov.3Environmental Microbial and Food Safety Laboratory, USDA Agricultural Research Service, Beltsville, MD 20705, United States.4Department of Nutrition and Food Science, University of Maryland, College Park, MD 20740, United States.AbstractBiofilm formation is a mechanism adapted by many microorganisms that enhances the survival in stressful environments. In food processing facilities, foodborne bacterial pathogens, which many are poor biofilm formers, could potentially take advantage of this protective mechanism by interacting with other strong biofilm producers. The objective of this study was to determine the influence of bacteria native to fresh produce processing environments on the incorporation of Escherichia coli O157:H7 in biofilms. Bacteria strains representing 13 Gram-negative species isolated from two fresh produce processing facilities in a previous study were tested for forming dual-species biofilms with E. coli O157:H7. Strong biofilm producing strains of Burkholderia caryophylli and Ralstonia insidiosa exhibited 180% and 63% increase in biofilm biomass, and significant thickening of the biofilms (B. caryophylli not tested), when co-cultured with E. coli O157:H7. E. coli O157:H7 populations increased by approximately 1 log in dual-species biofilms formed with B. caryophylli or R. insidiosa. While only a subset of environmental isolates with strong biofilm formation abilities increased the presence of E. coli O157:H7 in biofilms, all tested E. coli O157:H7 exhibited higher incorporation in dual-species biofilms with R. insidiosa. These observations support the notion that E. coli O157:H7 and specific strong biofilm producing bacteria interact synergistically in biofilm formation, and suggest a route for increased survival potential of E. coli O157:H7 in fresh produce processing environments.
- International journal of food microbiology.Int J Food Microbiol.2014 Feb 3;171:15-20. doi: 10.1016/j.ijfoodmicro.2013.11.007. Epub 2013 Nov 18.
- Biofilm formation is a mechanism adapted by many microorganisms that enhances the survival in stressful environments. In food processing facilities, foodborne bacterial pathogens, which many are poor biofilm formers, could potentially take advantage of this protective mechanism by interacting with o
- PMID 24296258
- PhaM Is the Physiological Activator of Poly(3-Hydroxybutyrate) (PHB) Synthase (PhaC1) in Ralstonia eutropha.
- Pfeiffer D, Jendrossek D.Author information Institute of Microbiology, University of Stuttgart, Stuttgart, Germany.AbstractPoly(3-hydroxybutyrate) (PHB) synthase (PhaC1) is the key enzyme of PHB synthesis in Ralstonia eutropha and other PHB-accumulating bacteria and catalyzes the polymerization of 3-hydroxybutyryl-CoA to PHB. Activity assays of R. eutropha PHB synthase are characterized by the presence of lag phases and by low specific activity. It is assumed that the lag phase is caused by the time necessary to convert the inactive PhaC1 monomer into the active dimeric form by an unknown priming process. The lag phase can be reduced by addition of nonionic detergents such as hecameg [6-O-(N-heptyl-carbamoyl)-methyl-α-d-glucopyranoside], which apparently accelerates the formation of PhaC1 dimers. We identified the PHB granule-associated protein (PGAP) PhaM as the natural primer (activator) of PHB synthase activity. PhaM was recently discovered as a novel type of PGAP with multiple functions in PHB metabolism. Addition of PhaM to PHB synthase assays resulted in immediate polymerization of 3HB coenzyme A with high specific activity and without a significant lag phase. The effect of PhaM on (i) PhaC1 activity, (ii) oligomerization of PhaC1, (iii) complex formation with PhaC1, and (iv) PHB granule formation in vitro and in vivo was shown by cross-linking experiments of purified proteins (PhaM, PhaC1) with glutardialdehyde, by size exclusion chromatography, and by fluorescence microscopic detection of de novo-synthesized PHB granules.
- Applied and environmental microbiology.Appl Environ Microbiol.2014 Jan;80(2):555-63. doi: 10.1128/AEM.02935-13. Epub 2013 Nov 8.
- Poly(3-hydroxybutyrate) (PHB) synthase (PhaC1) is the key enzyme of PHB synthesis in Ralstonia eutropha and other PHB-accumulating bacteria and catalyzes the polymerization of 3-hydroxybutyryl-CoA to PHB. Activity assays of R. eutropha PHB synthase are characterized by the presence of lag phases and
- PMID 24212577
- Metabolic engineering of Escherichia coli for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from glucose.
- Yang JE, Choi YJ, Lee SJ, Kang KH, Lee H, Oh YH, Lee SH, Park SJ, Lee SY.Author information Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program), Center for Systems and Synthetic Biotechnology, and Institute for the BioCentury, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon, 305-701, Republic of Korea.AbstractThe Escherichia coli XL1-blue strain was metabolically engineered to synthesize poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] through 2-ketobutyrate, which is generated via citramalate pathway, as a precursor for propionyl-CoA. Two different metabolic pathways were examined for the synthesis of propionyl-CoA from 2-ketobutyrate. The first pathway is composed of the Dickeya dadantii 3937 2-ketobutyrate oxidase or the E. coli pyruvate oxidase mutant (PoxB L253F V380A) for the conversion of 2-ketobutyrate into propionate and the Ralstonia eutropha propionyl-CoA synthetase (PrpE) or the E. coli acetyl-CoA:acetoacetyl-CoA transferase for further conversion of propionate into propionyl-CoA. The second pathway employs pyruvate formate lyase encoded by the E. coli tdcE gene or the Clostridium difficile pflB gene for the direct conversion of 2-ketobutyrate into propionyl-CoA. As the direct conversion of 2-ketobutyrate into propionyl-CoA could not support the efficient production of P(3HB-co-3HV) from glucose, the first metabolic pathway was further examined. When the recombinant E. coli XL1-blue strain equipped with citramalate pathway expressing the E. coli poxB L253F V380A gene and R. eutropha prpE gene together with the R. eutropha PHA biosynthesis genes was cultured in a chemically defined medium containing 20 g/L of glucose as a sole carbon source, P(3HB-co-2.3 mol% 3HV) was produced up to the polymer content of 61.7 wt.%. Moreover, the 3HV monomer fraction in P(3HB-co-3HV) could be increased up to 5.5 mol% by additional deletion of the prpC and scpC genes, which are responsible for the metabolism of propionyl-CoA in host strains.
- Applied microbiology and biotechnology.Appl Microbiol Biotechnol.2014 Jan;98(1):95-104. doi: 10.1007/s00253-013-5285-z. Epub 2013 Oct 11.
- The Escherichia coli XL1-blue strain was metabolically engineered to synthesize poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] through 2-ketobutyrate, which is generated via citramalate pathway, as a precursor for propionyl-CoA. Two different metabolic pathways were examined for the sy
- PMID 24113828
Japanese Journal
- Selective isolation of bacteria from soil with hydrophobic materials
- Oku Shota,Nishiyama Masaya,Takao Yuji
- World Journal of Microbiology and Biotechnology 27(8), 1941-1945, 2011-08
- … those closely related to Ralstonia pickettii, using ODS and DVB particles; …
- NAID 120002769905
- Ralstonia solanacearumの無病徴感染による感受性ナスの青枯病発病抑制
- 森 太郎,藤吉 智裕,稲田 達則,松崎 弘美,和島 孝浩,松添 直隆
- 農業生産技術管理学会誌 18(1), 23-28, 2011-06-15
- NAID 110008686411
Related Links
- Ralstonia is a genus of proteobacteria, previously included in the genus Pseudomonas. It is named after the American bacteriologist E. Ralston. Researchers at UCLA have genetically modified a variety of Ralstonia (Ralstonia eutropha H16) ...
- 2006年3月8日 ... 最近, “Ralstonia pickettii”についてMMWRの報告を読んだのですが, R. pickettiiは0.2 μmのフィルターも通過すると記載されていました。そこで教えていただきたいのですが, この細菌は人体にどのような影響を持っているのでしょうか??? また ...
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| リンク元 | 「ラルストニア」「ラルストニア属」 |
| 拡張検索 | 「Ralstonia eutropha」「Ralstonia solanacearum」「Ralstonia属」「Ralstoniaceae」 |