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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2013/02/20 01:53:15」(JST)
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- Partner Associations Across Sympatric Broad-headed Bug Species and Their Environmentally Acquired Bacterial Symbionts.
- Garcia JR, Laughton AM, Malik Z, Parker BJ, Trincot C, Chiang S, Chung E, Gerardo NM.Author information Biology Department, Emory University, O. Wayne Rollins Research Center, 1510 Clifton Rd, Atlanta, GA, 30322, USA.AbstractMany organisms have intimate associations with beneficial microbes acquired from the environment. These host-symbiont associations can be specific and stable, but they are prone to lower partner specificity and more partner switching than vertically transmitted mutualisms. To investigate partner specificity in an environmentally acquired insect symbiosis, we used 16S rRNA gene and multilocus sequencing to survey the bacterial population in the bacteria-harboring organ (crypts) of 49 individuals across four sympatric broad-headed bug species (Alydus calcaratus, A. conspersus, A. tomentosus, Megalotomus quinquespinosus). Similar to other insect-bacteria associations, Burkholderia spp. were the most common residents of the crypts in all four insect species (77.2% of recovered sequences), and Burkholderia presence was associated with prolonged survival to adulthood in A. tomentosus, suggesting a beneficial role of these specialized associations. Burkholderia were also found in environmental reservoirs in the insects' habitat, which may facilitate acquisition by insects by increasing Burkholderia - insect encounters. Symbiont establishment could also be facilitated by resistance to insect defenses; zone of inhibition assays demonstrated that Burkholderia and other bacteria isolated from crypts are resistant to insect defenses that limit growth of Escherichia coli. Alternatively, the insects' defenses may not efficiently kill a broad range of bacteria. Although the symbiosis is targeted to Burkholderia, the insects' crypts housed other bacteria, including non-Burkholderiaceae species. There is no significant effect of host insect species on Burkholderia distribution, suggesting a lack of strong partner specificity at finer scales. The presence of frequent partner switching between sympatric insects and their symbionts likely prevents tight co-evolutionary dynamics. This article is protected by copyright. All rights reserved.
- Molecular ecology.Mol Ecol.2014 Jan 2. doi: 10.1111/mec.12655. [Epub ahead of print]
- Many organisms have intimate associations with beneficial microbes acquired from the environment. These host-symbiont associations can be specific and stable, but they are prone to lower partner specificity and more partner switching than vertically transmitted mutualisms. To investigate partner spe
- PMID 24384031
- Correlates of immune protection following cutaneous immunization with an attenuated Burkholderia pseudomallei vaccine.
- Silva EB, Goodyear A, Sutherland MD, Podnecky NL, Gonzalez-Juarrero M, Schweizer HP, Dow SW.Author information Rocky Mountain Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research and Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado, USA.AbstractInfections with the Gram-negative bacterium Burkholderia pseudomallei (melioidosis) are associated with high mortality, and there is currently no approved vaccine to prevent the development of melioidosis in humans. Infected patients also do not develop protective immunity to reinfection, and some individuals will develop chronic, subclinical infections with B. pseudomallei. At present, our understanding of what constitutes effective protective immunity against B. pseudomallei infection remains incomplete. Therefore, we conducted a study to elucidate immune correlates of vaccine-induced protective immunity against acute B. pseudomallei infection. BALB/c and C57BL/6 mice were immunized subcutaneously with a highly attenuated, Select Agent-excluded purM deletion mutant of B. pseudomallei (strain Bp82) and then subjected to intranasal challenge with virulent B. pseudomallei strain 1026b. Immunization with Bp82 generated significant protection from challenge with B. pseudomallei, and protection was associated with a significant reduction in bacterial burden in lungs, liver, and spleen of immunized mice. Humoral immunity was critically important for vaccine-induced protection, as mice lacking B cells were not protected by immunization and serum from Bp82-vaccinated mice could transfer partial protection to nonvaccinated animals. In contrast, vaccine-induced protective immunity was found to be independent of both CD4 and CD8 T cells. Tracking studies demonstrated uptake of the Bp82 vaccine strain predominately by neutrophils in vaccine-draining lymph nodes and by smaller numbers of dendritic cells (DC) and monocytes. We concluded that protection following cutaneous immunization with a live attenuated Burkholderia vaccine strain was dependent primarily on generation of effective humoral immune responses.
- Infection and immunity.Infect Immun.2013 Dec;81(12):4626-34. doi: 10.1128/IAI.00915-13. Epub 2013 Oct 7.
- Infections with the Gram-negative bacterium Burkholderia pseudomallei (melioidosis) are associated with high mortality, and there is currently no approved vaccine to prevent the development of melioidosis in humans. Infected patients also do not develop protective immunity to reinfection, and some i
- PMID 24101688
- Structures of intermediate transport states of ZneA, a Zn(II)/proton antiporter.
- Pak JE, Ekendé EN, Kifle EG, O'Connell JD 3rd, De Angelis F, Tessema MB, Derfoufi KM, Robles-Colmenares Y, Robbins RA, Goormaghtigh E, Vandenbussche G, Stroud RM.Author information Department of Biochemistry and Biophysics, Center for the Structure of Membrane Proteins, Membrane Protein Expression Center, University of California, San Francisco, CA 94158.AbstractEfflux pumps belonging to the ubiquitous resistance-nodulation-cell division (RND) superfamily transport substrates out of cells by coupling proton conduction across the membrane to a conformationally driven pumping cycle. The heavy metal-resistant bacteria Cupriavidus metallidurans CH34 relies notably on as many as 12 heavy metal efflux pumps of the RND superfamily. Here we show that C. metallidurans CH34 ZneA is a proton driven efflux pump specific for Zn(II), and that transport of substrates through the transmembrane domain may be electrogenic. We report two X-ray crystal structures of ZneA in intermediate transport conformations, at 3.0 and 3.7 Å resolution. The trimeric ZneA structures capture protomer conformations that differ in the spatial arrangement and Zn(II) occupancies at a proximal and a distal substrate binding site. Structural comparison shows that transport of substrates through a tunnel that links the two binding sites, toward an exit portal, is mediated by the conformation of a short 14-aa loop. Taken together, the ZneA structures presented here provide mechanistic insights into the conformational changes required for substrate efflux by RND superfamily transporters.
- Proceedings of the National Academy of Sciences of the United States of America.Proc Natl Acad Sci U S A.2013 Nov 12;110(46):18484-9. doi: 10.1073/pnas.1318705110. Epub 2013 Oct 30.
- Efflux pumps belonging to the ubiquitous resistance-nodulation-cell division (RND) superfamily transport substrates out of cells by coupling proton conduction across the membrane to a conformationally driven pumping cycle. The heavy metal-resistant bacteria Cupriavidus metallidurans CH34 relies nota
- PMID 24173033
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★リンクテーブル★
| リンク元 | 「バークホルデリア属」「ラルストニア科」「Burkholderiaceae」 |
「バークホルデリア属」
- ラ
- Burkholderia
- 同
- ブルクホルデリア属、ブルクホルデリア、バークホルデリア、Burkholderia属
- 関
- グラム陰性桿菌、Burkholderiaceae、Ralstoniaceae、バークホルデリア科、ラルストニア科
- 類鼻疽 Burkholderia pseudomallei
- 嚢胞性線維症における感染症 Burkholderia cepacia complex