pH緩衝剤

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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2014/06/12 23:16:45」(JST)

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PIPES is the common name for piperazine-N,N′-bis(2-ethanesulfonic acid), and frequently used buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s.^[1]

Applications

PIPES has pKa (6.76 at 25°C) near the physiological pH which makes it useful in cell culture work. PIPES has been documented minimizing lipid loss when buffering glutaraldehyde histology in plant and animal tissues.^[2]^[3] Fungal zoospore fixation for fluorescence microscopy and electron microscopy were optimized with a combination of glutaraldehyde and formaldehyde in PIPES buffer.^[4] It has a negligible capacity to bind divalent ions.

References

^ Good, Norman E.; Winget, G. Douglas; Winter, Wilhelmina; Connolly, Thomas N.; Izawa, Seikichi; Singh, Raizada M. M. (1966). "Hydrogen Ion Buffers for Biological Research". Biochemistry 5 (2): 467–77. doi:10.1021/bi00866a011. PMID 5942950.
^ Salema, R. and Brando, I., J. Submicr. Cytol., 9, 79 (1973).
^ Schiff, R.I. and Gennaro, J.F., Scaning Electron Microsc., 3, 449 (1979).
^ Hardham, A.R. (1985). "Studies on the cell surface of zoospores and cysts of the fungus Phytophthora cinnamomi: The influence of fixation on patterns of lectin binding". Journal of Histochemistry 33 (2): 110–8. doi:10.1177/33.2.3918095. PMID 3918095.

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「PIP」

PIPES
	IUPAC name 1,4-Piperazinediethanesulfonic acid (IUPAC)
	Other names PIPES
Identifiers
CAS number	5625-37-6
PubChem	6992709
ChemSpider	72022
Jmol-3D images	Image 1
	SMILES C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O ;
	InChI InChI=1S/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16) ; Key: IHPYMWDTONKSCO-UHFFFAOYSA-N InChI=1/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16); Key: IHPYMWDTONKSCO-UHFFFAOYAG ;
Properties
Molecular formula	C8H18N2O6S2
Molar mass	302.37
Appearance	White powder
Melting point	Decomposes above 300 °C
Boiling point	Decomposes
Solubility in water	1 g/L (100 °C)
Hazards
MSDS	External MSDS
Main hazards	Irritant
NFPA 704	0 1 0
	Except where noted otherwise, data are given for materials in their standard state (at 25 °C (77 °F), 100 kPa)
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	Infobox references

　　[★]

[1] Good, Norman E.; Winget, G. Douglas; Winter, Wilhelmina; Connolly, Thomas N.; Izawa, Seikichi; Singh, Raizada M. M. (1966). "Hydrogen Ion Buffers for Biological Research". Biochemistry 5 (2): 467–77. doi:10.1021/bi00866a011. PMID 5942950.

[2] Salema, R. and Brando, I., J. Submicr. Cytol., 9, 79 (1973).

[3] Schiff, R.I. and Gennaro, J.F., Scaning Electron Microsc., 3, 449 (1979).

[4] Hardham, A.R. (1985). "Studies on the cell surface of zoospores and cysts of the fungus Phytophthora cinnamomi: The influence of fixation on patterns of lectin binding". Journal of Histochemistry 33 (2): 110–8. doi:10.1177/33.2.3918095. PMID 3918095.

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PIPES