高性能薄層クロマトグラフィー, high-performance thin-layer chromatography
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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2016/02/17 05:16:49」(JST)
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Analysis of food-dyes: (A) photo of HPTLC plate (developed from both sides), (B) multi-wavelength scan of mix 1, (C) calibration function, (D) mass spectra of selected zones, (E) results
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High performance thin layer chromatography (HPTLC) is an enhanced form of thin layer chromatography (TLC). A number of enhancements can be made to the basic method of thin layer chromatography to automate the different steps, to increase the resolution achieved and to allow more accurate quantitative measurements.
Automation is useful to overcome the uncertainty in droplet size and position when the sample is applied to the TLC plate by hand. One recent approach to automation has been the use of piezoelectric devices and inkjet printers for applying the sample.[2]
The spot capacity (analogous to peak capacity in HPLC) can be increased by developing the plate with two different solvents, using two-dimensional chromatography.[3] The procedure begins with development of sample loaded plate with first solvent. After removing it, the plate is rotated 90° and developed with a second solvent.
Instrumentation
Widely used instrument for HPTLC is from CAMAG,[4] Switzerland. It provides automated sample application (loading), plate development, detection and documentation.[5]
References
- Notes
- ^ G. Morlock, C. Oellig (2009), CAMAG Bibliography Service 103, 5
- ^ Morlock, Gertrud E.; Claudia Oellig, Louis W. Bezuidenhout, Michael J. Brett & Wolfgang Schwack (2010). "Miniaturized planar chromatography using office peripherals". Analytical Chemistry 82 (7): 2940–2946. doi:10.1021/ac902945t.
- ^ Nurok, David (1989). "Strategies for optimizing the mobile phase in planar chromatography". Chemical Reviews 89 (2): 363–375. doi:10.1021/cr00092a007.
- ^ "TLC / HPTLC, DBS, Alox - Made in Switzerland". CAMAG. Retrieved 2014-08-16.
- ^ "What is TLC/HPTLC". CAMAG. Retrieved 2014-08-16.
- Sources
- Puri, A., Ahmad, A. and Panda, B. P. (2010), Development of an HPTLC-based diagnostic method for invasive aspergillosis. Biomed. Chromatogr., 24: 887–892. doi: 10.1002/bmc.1382 [1]
- Ajaz Ahmad, M Mujeeb, Bibhu Prasad Panda (2010) An HPTLC Method for the Simultaneous Analysis of Compactin and Citrinin in Penicillium citrinum Fermentation Broth. Journal of Planar Chromatography-modern TLC 23 (4), 282–285 [2]
Bibliography
- F. Geiss (1987): Fundamentals of thin layer chromatography planar chromatography, Heidelberg, Hüthig, ISBN 3-7785-0854-7
- Jork, H., Funk, W., Fischer, W., Wimmer, H. (1990): Thin-Layer Chromatography: Reagents and Detection Methods, Volume 1a, VCH, Weinheim, ISBN 3-527-27834-6
- Jork, H., Funk, W., Fischer, W., Wimmer, H. (1994): Thin-Layer Chromatography: Reagents and Detection Methods, Volume 1b, VCH, Weinheim
- Hahn-Deinstorp, E. (2000): Applied Thin-Layer Chromatography. Best Practice and Avoidance of Mistakes, Wiley-VCH, Weinheim, ISBN 3-527-29839-8
- Spangenberg, B., Poole, C. F., Weins, C. (2011): Quantitative Thin-Layer Chromatography : A Practical Survey, Springer, Berlin, Heidelberg
- Zlatkis, A., Kaiser, RE. (editors), (1977): HPTLC : high performance thin-layer chromatography, Amsterdam, Elsevier
- Reich A., Schibli A. (2006): High Performance Thin-Layer Chromatography for the Analysis of Medicinal Plants, Thieme, New York, Stuttgart, ISBN 1-58890-409-1, ISBN 978-1-58890-409-6
- Sethi, P. D. (2011): Sethi’s HPTLC, High Performance Thin Layer Chromatography. Content Uniformity of Pharmaceutical Formulations. Kongposh Publications, New Delhi, ISBN 978-81-906264-3-9
- Gupta, A. K., Tandon, N., Sharma, M. (2003-2012): Quality Standards of Indian Medicinal Plants, volume 1-10,ICMR, New Delhi, ISSN 0972-7213
- Jaiswal, P.K. (2010): High Performance Thin Layer Chromatography in Food Analysis, CBS Publisher & Distributors, New Delhi, ISBN 978-81-239-1837-2
- Waksmundzka-Hajnos, M., Sherma, J., Kowalska, T. (2008): Thin Layer Chromatography in Phytochemistry, CRC Press (Taylor & Francis Group), Boca Raton, ISBN 978-1-4200-4677-9
- Touchstone, J. C. (1985): Techniques and Applications of Thin Layer Chromatography, John Wiley & Sons, Inc. New York, ISBN 0471880175, 9780471880172
- Tandon, N. (2010) Phytochemical Reference Standards of Selected Indian Medicinal Plants, volume 1, ICMR, New Delhi, ISSN 0976-6057
- Srivastava M. M. (2011) High Performance thin Layer Chromatography, Springer Verlag, Heidelberg, ISBN 978-3-642-14024-2
- Sethi P. D. (2013), Sethi’s HPTLC, High Performance Thin Layer Chromatography. Quantitative Analysis of Pharmaceutical Formulations. Volumes 1-3, CBS Publishers & Distributors, New Delhi, India ISBN 978-81-239-2279-9
English Journal
- A new high-performance thin layer chromatography-based assay of detergents and surfactants commonly used in membrane protein studies.
- Barret LA, Polidori A, Bonneté F, Bernard-Savary P, Jungas C.SourceCEA, IBEB, Lab Bioenerget Cellulaire, Saint-Paul-lez-Durance, F-13108, France; CNRS, UMR 7265 Biol Veget & Microbiol Environ, Saint-Paul-lez-Durance, F-13108, France; Aix-Marseille Univ, Saint-Paul-lez-Durance, F-13108, France; Université d'Avignon et des pays du Vaucluse, Equipe Chimie Bioorganique et Systèmes Amphiphiles, 33 rue Louis Pasteur, F 84000 Avignon, France; Institut des Biomolécules Max Mousseron, UMR 5247, CNRS-Universités Montpellier 1&2, 15 avenue Charles Flahaut, F-34093 Montpellier Cedex 05, France.
- Journal of chromatography. A.J Chromatogr A.2013 Mar 15;1281C:135-141. doi: 10.1016/j.chroma.2013.01.061. Epub 2013 Jan 24.
- The hydrophobic nature of membrane proteins (MPs) necessitates the use of detergents for their extraction, solubilization and purification. Because the concentration of amphiphiles is crucial in the crystallization process, detergent quantification is essential to routine analysis. Here we describe
- PMID 23398993
- Isolation and characterization of hyaluronic acid from the liver of marine stingray Aetobatus narinari.
- Sadhasivam G, Muthuvel A, Pachaiyappan A, Thangavel B.SourceFaculty of Marine Sciences, Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai 608502, Tamil Nadu, India. Electronic address: gijisadasivam@gmail.com.
- International journal of biological macromolecules.Int J Biol Macromol.2013 Mar;54:84-9. doi: 10.1016/j.ijbiomac.2012.11.028. Epub 2012 Dec 7.
- Although hyaluronic acid research pursuits ahead in exploring its biomedical perspective, very limited investigations were carried out in their isolation shape view point, furthermore, most of the investigations were targeted towards the terrestrial source. To swerve from that, the present study was
- PMID 23220595
- Validated HPLC and HPTLC Methods for Simultaneous Determination of Colchicine and Khellin in Pharmaceutical Formulations.
- Hadad GM, Badr JM, El-Nahriry K, Hassanean HA.Source1Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, University of Suez Canal, Ismailia 41522, Egypt.
- Journal of chromatographic science.J Chromatogr Sci.2013 Mar;51(3):258-65. doi: 10.1093/chromsci/bms135. Epub 2012 Aug 21.
- The present work describes validated high-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) methods for the simultaneous determination of colchicine and khellin. The isocratic reversed-phase HPLC separation was performed on a 5 µm C18 column (Luna, Phen
- PMID 22914568
Japanese Journal
- 4,8-Sphingadienine and 4-hydroxy-8-sphingenine activate ceramide production in the skin
- Shirakura Yoshiyuki,Kikuchi Kanako,Matsumura Kenji,Mukai Katsuyuki,Mitsutake Susumu,Igarashi Yasuyuki
- Lipids in Health and Disease 11, 108, 2012-08-31
- … The effect of d18:2 and t18:1 on stimulating ceramide production was evaluated using HPTLC analysis in a 3-dimensional human skin model. …
- NAID 120004979562
- Effect of saponification on composition of unsaponifiable matter in rice bran oil
- Deepam L. S. Afinisha,Arumughan C.
- Journal of Oleo Science 61(5), 241-247, 2012-05-00
- NAID 40019229931
- Effects of Blanching Method on Sugar and Protodioscin Contents of White Asparagus Spears
- Maeda Tomoo,Jishi Tatsuru,Honda Kazushige [他]
- Journal of the Japanese Society for Horticultural Science 81(2), 166-170, 2012-04-00
- NAID 40019226534
Related Links
- HPTLC 2014 : International Symposium for Thin-Layer Chromatography, Lyon france LYON, 02 nd-04 th July 2014 Thin-Layer Chromatography (TLC) is a form of liquid chromatography utilizing a flat open separation ...
- Manufacturer of densitometer and systems for TLC, HPTLC and planar chromatography. Assembly overviews, contacts for distributors, subsidiaries in Germany and USA, headquartered in Muttenz, Switzerland.
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