somatic cell nuclear transplantation

　

WordNet

1. moving a cell nucleus and its genetic material from one cell to another (同)somatic cell nuclear transfer, SCNT, nuclear_transplantation
2. small room in which a monk or nun lives (同)cubicle
3. a device that delivers an electric current as the result of a chemical reaction (同)electric cell
4. a room where a prisoner is kept (同)jail cell, prison cell
5. (biology) the basic structural and functional unit of all organisms; they may exist as independent units of life (as in monads) or may form colonies or tissues as in higher plants and animals
6. any small compartment; "the cells of a honeycomb"
7. a small unit serving as part of or as the nucleus of a larger political movement (同)cadre
8. of or relating to or constituting the nucleus of an atom; "nuclear physics"; "nuclear fission"; "nuclear forces"
9. (weapons) deriving destructive energy from the release of atomic energy; "nuclear war"; "nuclear weapons"; "atomic bombs" (同)atomic
10. constituting or like a nucleus; "annexation of the suburban fringe by the nuclear metropolis"; "the nuclear core of the congregation"
11. of or relating to or constituting the nucleus of a cell; "nuclear membrane"; "nuclear division"

PrepTutorEJDIC

1. (刑務所の)『独房』;(修道院の)小さい独居室 / (ミツバチの)みつ房,巣穴 / 小さい部屋 / 『細胞』 / 電池 / 花粉室 / (共産党などの)細胞
2. 『核の』,細胞核の / [『原子』]『核の』
3. 移植;移植した物 / 移住 / (内臓などの)移植術
4. 身体の / 体腔(たいこう)の / 体細胞の

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• 1. 身体化：疫学、病因、臨床的特徴、医学的評価、および診断 somatization epidemiology pathogenesis clinical features medical evaluation and diagnosis
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• 5. 本態性環境不耐性（多種化学物質過敏症）の概要 overview of idiopathic environmental intolerance multiple chemical sensitivity

English Journal

• Ovum pick up, intracytoplasmic sperm injection and somatic cell nuclear transfer in cattle, buffalo and horses: from the research laboratory to clinical practice.

• Galli C, Duchi R, Colleoni S, Lagutina I, Lazzari G.Author information Avantea, Laboratory of Reproductive Technologies, 26100 Cremona, Italy; Department of Veterinary Medical Sciences, University of Bologna, Italy; Fondazione Avantea, Cremona, Italy. Electronic address: cesaregalli@avantea.it.AbstractAssisted reproductive techniques developed for cattle in the last 25 years, like ovum pick up (OPU), intracytoplasmic sperm injection (ICSI), and somatic cell nuclear transfer, have been transferred and adapted to buffalo and horses. The successful clinical applications of these techniques require both the clinical skills specific to each animal species and an experienced laboratory team to support the in vitro phase of the work. In cattle, OPU can be considered a consolidated technology that is rapidly outpacing conventional superovulation for embryo transfer. In buffalo, OPU represents the only possibility for embryo production to advance the implementation of embryo-based biotechnologies in that industry, although it is still mainly in the developmental phase. In the horse, OPU is now an established procedure for breeding from infertile and sporting mares throughout the year. It requires ICSI that in the horse, contrary to what happens in cattle and buffalo, is very efficient and the only option because conventional IVF does not work. Somatic cell nuclear transfer is destined to fill a very small niche for generating animals of extremely high commercial value. The efficiency is low, but because normal animals can be generated it is likely that advancing our knowledge in that field might improve the technology and reduce its cost.
• Theriogenology.Theriogenology.2014 Jan 1;81(1):138-51. doi: 10.1016/j.theriogenology.2013.09.008.
• Assisted reproductive techniques developed for cattle in the last 25 years, like ovum pick up (OPU), intracytoplasmic sperm injection (ICSI), and somatic cell nuclear transfer, have been transferred and adapted to buffalo and horses. The successful clinical applications of these techniques require b
• PMID 24274418

• Production of diabetic offspring using cryopreserved epididymal sperm by in vitro fertilization and intrafallopian insemination techniques in transgenic pigs.

• Umeyama K, Honda K, Matsunari H, Nakano K, Hidaka T, Sekiguchi K, Mochizuki H, Takeuchi Y, Fujiwara T, Watanabe M, Nagaya M, Nagashima H.Author information Laboratory of Developmental Engineering, Department of Life Sciences, School of Agriculture, Meiji University, Kanagawa 214-8571, Japan.AbstractSomatic cell nuclear transfer (SCNT) is a useful technique for creating pig strains that model human diseases. However, production of numerous cloned disease model pigs by SCNT for large-scale experiments is impractical due to its complexity and inefficiency. In the present study, we aimed to establish an efficient procedure for proliferating the diabetes model pig carrying the mutant human hepatocyte nuclear factor-1α gene. A founder diabetes transgenic cloned pig was generated by SCNT and treated with insulin to allow for normal growth to maturity, at which point epididymal sperm could be collected for cryopreservation. In vitro fertilization and intrafallopian insemination using the cryopreserved epididymal sperm resulted in diabetes model transgenic offspring. These results suggest that artificial reproductive technology using cryopreserved epididymal sperm could be a practical option for proliferation of genetically modified disease model pigs.
• The Journal of reproduction and development.J Reprod Dev.2013 Dec 17;59(6):599-603. Epub 2013 Aug 24.
• Somatic cell nuclear transfer (SCNT) is a useful technique for creating pig strains that model human diseases. However, production of numerous cloned disease model pigs by SCNT for large-scale experiments is impractical due to its complexity and inefficiency. In the present study, we aimed to establ
• PMID 23979397

• RNAi-based inhibition of porcine reproductive and respiratory syndrome virus replication in transgenic pigs.

• Li L1, Li Q1, Bao Y2, Li J1, Chen Z1, Yu X3, Zhao Y1, Tian K4, Li N5.Author information 1State Key Laboratories of Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing 100193, China; Department of Molecular Biology, College of Biological Science, China Agricultural University, Beijing 100193, China.2Department of Basic Immunology, Xinxiang Medical University, Xinxiang 453003, China.3OIE Porcine Reproductive and Respiratory Syndrome Reference Laboratory, China Animal Disease Control Center, No. 20 Maizidian Road, Chaoyang District, Beijing 100125, China.4OIE Porcine Reproductive and Respiratory Syndrome Reference Laboratory, China Animal Disease Control Center, No. 20 Maizidian Road, Chaoyang District, Beijing 100125, China; National Research Center for Veterinary Medicine, Cuiwei Road, High-Tech. District, Luoyang, Henan Provice, China.5State Key Laboratories of Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing 100193, China; Department of Molecular Biology, College of Biological Science, China Agricultural University, Beijing 100193, China. Electronic address: ninglcau@cau.edu.cn.AbstractPorcine reproductive and respiratory syndrome (PRRS) is an economically devastating viral disease causing heavy losses to the swine industry worldwide. Many studies have shown that transient delivery of small interfering RNA (siRNA) or adenovirus-mediated RNA interfere (RNAi) could potentially inhibit porcine reproductive and respiratory syndrome virus (PRRSV) replication in vivo and in vitro. Here, we applied RNAi to produce transgenic (TG) pigs that constitutively expressed PRRSV-specific siRNA derived from small hairpin RNA (shRNA). First, we evaluated siRNA expression in the founding and F1 generation pigs and confirmed stable transmission. Then, we detected the expression of IFN-β and protein kinase R (PKR) and found no difference among TG, non-transgenic (NTG), and wild-type pigs. Lastly, the F1 generation pigs, including TG and NTG piglets, were challenged with 3×104.5 TCID50 of JXA1, a highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV). Our results showed that the in vivo siRNA expression substantially reduced the serum HP-PRRSV titers and increased survival time by 3 days when TG pigs were compared with the NTG controls. These data suggested that RNAi-based genetic modification might be used to breed viral-resistant livestock with stable siRNA expression with no complications of siRNA toxicity.
• Journal of biotechnology.J Biotechnol.2013 Dec 11. pii: S0168-1656(13)00527-0. doi: 10.1016/j.jbiotec.2013.11.022. [Epub ahead of print]
• Porcine reproductive and respiratory syndrome (PRRS) is an economically devastating viral disease causing heavy losses to the swine industry worldwide. Many studies have shown that transient delivery of small interfering RNA (siRNA) or adenovirus-mediated RNA interfere (RNAi) could potentially inhib
• PMID 24333125

Japanese Journal

• Ascorbic Acid Improves the Developmental Competence of Porcine Oocytes After Parthenogenetic Activation and Somatic Cell Nuclear Transplantation

• KERE Michel,SIRIBOON Chawalit,LO Neng-Wen,NGUYEN Ngoc Tan,JU Jyh-Cherng
• The Journal of reproduction and development 59(1), 78-84, 2013-02-01
• NAID 10031156815

• Ascorbic Acid Improves the Developmental Competence of Porcine Oocytes After Parthenogenetic Activation and Somatic Cell Nuclear Transplantation

• KERE Michel,SIRIBOON Chawalit,LO Neng-Wen [他],NGUYEN Ngoc Tan,JU Jyh-Cherng
• Journal of Reproduction and Development 59(1), 78-84, 2013
• … None of these vitamin C additions affected nuclear maturation of oocytes, yet supplementation at 50 μg/ml led to significantly increased intracellular glutathione (GSH) levels and reduced reactive oxygen species (ROS). … When cultured in IVM- and/or IVC-supplemented media, the group supplemented with 50 μg/ml of vitamin C showed improved cleavage rates, blastocyst rates and total cell numbers per blastocyst (P<0.05) compared with other groups (control, 25 μg/ml and 100 μg/ml). …
• NAID 130002475120

• Technical Development for Production of Gene-Modified Laboratory Rats

• Hirabayashi Masumi
• The Journal of reproduction and development 54(2), 95-99, 2008-04-01
• … Transgenic rats have been used as model animals for human diseases and organ transplantation and as animal bioreactors for protein production. … For successful production of transgenic rats with a modified endogenous gene, establishment of embryonic stem cell lines or alternatively male germline stem cell lines and technical development of somatic cell nuclear transfer are still necessary for this species. …
• NAID 10021218742

Related Links

• Somatic-cell nuclear transfer - Wikipedia, the free encyclopedia

In genetics and developmental biology, somatic-cell nuclear transfer (SCNT) is a laboratory technique for creating a clone embryo with a donor nucleus (see process below). It can be used in embryonic stem cell research, or, potentially, ...

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★リンクテーブル★

関連記事	「transplantation」「somatic」「nuclear」「cell」「somatic cell」

「transplantation」

　　[★]

• n.

• 移植、移植術

関

cell transplant、engraft、engraftment、graft、grafting、implant、implantation、organ graft、organ transplant、tissue graft、tissue transplant、transfer、transplant、transplant recipient

　

「somatic」

　　[★]

• adj.

• 体細胞の、体性の、細胞体の、体部の、体細胞性の

関

cell body、soma、somata、somatic cell、somatically

　

「nuclear」

　　[★]

• adj.

• 核の、(生物)細胞核の、(物理)原子核の

関

cell nuclei、cell nucleus、nuclei、nucleo、nucleus

　 　 　 　 　

「cell」

　　[★] 細胞 　 　 　 　 　 　 　

「somatic cell」

　　[★]

• 体細胞

関

somatic