isoelectric focusing

出典: meddic

IEF&antibody heterogeneity

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出典(authority):フリー百科事典『ウィキペディア(Wikipedia)』「2012/07/10 23:18:39」(JST)

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  • Mapping of Epitopes Occurring in Bovine αs1-Casein Variants by Peptide Microarray Immunoassay.
  • Lisson M1, Erhardt G2.
  • Methods in molecular biology (Clifton, N.J.).Methods Mol Biol.2016;1352:279-96. doi: 10.1007/978-1-4939-3037-1_21.
  • Immunoglobulin E epitope mapping of milk proteins reveals important information about their immunologic properties. Genetic variants of αS1-casein, one of the major allergens in bovine milk, are until now not considered when discussing the allergenic potential. Here we describe the complete procedu
  • PMID 26490483
  • Use of isoelectric focusing to discriminate transient oligoclonal bands from monoclonal protein in treated myeloma.
  • Henry R, Glegg D.
  • Clinical chemistry and laboratory medicine : CCLM / FESCC.Clin Chem Lab Med.2015 Dec 7. pii: /j/cclm.ahead-of-print/cclm-2015-0642/cclm-2015-0642.xml. doi: 10.1515/cclm-2015-0642. [Epub ahead of print]
  • A 62-year-old diabetic man with prostate cancer first presented to our clinical laboratory in 2003 with a normal serum protein electrophoresis and immunofixation. In March 2009 he was diagnosed with an IgG κ myeloma. He underwent treatment and went into remission with the original paraprotein band
  • PMID 26641967
  • Structural Characterization of a Monoclonal Antibody (mAb) - Maytansinoid Immunoconjugate.
  • Luo Q, Chung HH, Borths CJ, Janson M, Wen J, Joubert MK, Wypych J.
  • Analytical chemistry.Anal Chem.2015 Dec 2. [Epub ahead of print]
  • Structural characterization was performed on an antibody drug conjugate (ADC), composed of an IgG1 monoclonal antibody (mAb), mertansine drug (DM1) and a non-cleavable linker. The DM1 molecules were conjugated through non-specific modification of the mAb at solvent exposed lysine residues. Due to th
  • PMID 26629796


  • Establishment of the Optimal Conditions for Two–Dimensional Gel Electrophoresis of Papaya Seed Proteome
  • Roan Su–Feng,Hsiung Tung–Chuan,Yang Kai–Yun,Liu Wei–Ting,Chang Ing–Feng,Wu Chun–Ta,Wakana Akira,若菜 章
  • Journal of the Faculty of Agriculture, Kyushu University 58(2), 239-246, 2013-09
  • … The resolution of 2D gel analysis for papaya seed proteome was further improved by utilizing pH 4-7 isoelectric focusing gel instead of pH 3-10 one. …
  • NAID 120005326648
  • Development of a highly sensitive three-dimensional gel electrophoresis method for characterization of monoclonal protein heterogeneity
  • Nakano Keiichi,Tamura Shogo,Otuka Kohei,Niizeki Noriyasu,Shigemura Masahiko,Shimizu Chikara,Matsuno Kazuhiko,Kobayashi Seiichi,Moriyama Takanori
  • Analytical Biochemistry 438(2), 117-123, 2013-07-15
  • … Three-dimensional gel electrophoresis (3-DE), which combines agarose gel electrophoresis and isoelectric focusing/SDS–PAGE, was developed to characterize monoclonal proteins (M-proteins). …
  • NAID 120005311827
  • Separation and characterization of the constituents of compost and soil humic acids by two-dimensional electrophoresis(Soil chemistry and soil mineralogy)
  • KARIM Saiful,OKUYAMA Yuji,AOYAMA Masakazu
  • Soil science and plant nutrition 59(2), 130-141, 2013-04
  • … In this method, HAs are first separated by isoelectric focusing (IEF) and then separated by polyacrylamide gel electrophoresis (PAGE). … Upon 2-D electrophoresis of HAs, dark-colored substances were spread out across the gel mainly in the isoelectric point (pI) range of 3.0-4.5. …
  • NAID 110009688303


isoelectric focusing 等電点電気泳動法 - アルクがお届けする進化するオンライン英和・和英辞書データベース。一般的な単語や連語から、イディオム、専門用語、スラングまで幅広く収録。
AES Application Focus Isoelectric Focusing Page 4 electrolyte strips. IPG strips for 2-D PAGE are often run with the gel facing down in dedicated IEF cells. Protocol 1. Casting gels for isoelectric focusing Equipment and ...


isoelectric focusing figure the principle Isoelectric focusingIsoelectric focusing (Electrofocusing) : Principle of Isoelectric Focusing (IEFIsoelectric focusing (Electrofocusing)Isoelectric Focusing | National

拡張検索gradient gel isoelectric focusing」「column isoelectric focusing



isoelectric focusingIEFchromatofocusingelectrofocusing



electrofocusingIEFisoelectric focusing



chromatofocusingIEFisoelectric focusing



chromatofocusingelectrofocusingisoelectric focusing



isoelectric focusingelectrofocusing

gradient gel isoelectric focusing」

  [★] 密度勾配等電点電気泳動法

column isoelectric focusing」

  [★] カラム等電点電気泳動法



  • 等電点の、等電の
isoelectric pointpI