A method for using direct injection of plasmid DNA to study cis-regulatory element activity in F0 Xenopus embryos and tadpoles.
Wang C1, Szaro BG2.
Developmental biology.Dev Biol.2015 Feb 1;398(1):11-23. doi: 10.1016/j.ydbio.2014.11.010. Epub 2014 Nov 20.
The ability to express exogenous reporter genes in intact, externally developing embryos, such as Xenopus, is a powerful tool for characterizing the activity of cis-regulatory gene elements during development. Although methods exist for generating transgenic Xenopus lines, more simplified methods fo
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