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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2013/04/26 15:22:11」(JST)

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CD146 (cluster of differentiation 146) also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. In humans, the CD146 protein is encoded by the MCAM gene.^[1]

Function

Its function is still poorly understood, but evidence points to it being part of the endothelial junction associated with the actin cytoskeleton. A member of the Immunoglobulin superfamily, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. It is expressed on chicken embryonic spleen and thymus, activated human T cells, endothelial progenitors such as angioblasts and mesenchymal stem cells, and strongly expressed on blood vessel endothelium and smooth muscle.

Two isoforms exist (MCAM long (MCAM-1), and MCAM short, or MCAM-s) which differ in the length of their cytoplasmic domain. Activation of these isoforms seems to produce functional differences as well. Natural killer cells transfected with MCAM-1 demonstrate decreased rolling velocity and increased cell adhesion to an endothelial cell monolayer and increased microvilli formation while cells transfected with MCAM-s showed no change in adhesion characteristics. Since these characteristics are important in leukocyte extravasation, MCAM-1 may be an important part of the inflammatory response.

CD146 has been demonstrated to appear on a small subset of T and B lymphocytes in the peripheral blood of healthy individuals. The CD146+ T cells display an immunophenotype consistent with effector memory cells and have a distinct gene profile from the CD146- T cells.^[2]^[3]

CD146 has been seen as a marker for mesenchymal stem cells isolated from multiple adult and fetal organs,^[4] and its expression may be linked to multipotency; mesenchymal stem cells with greater differentiation potential express higher levels of CD146 on the cell surface.^[5]

References

^ Kuske MD, Johnson JP (1999). "Assignment of the human melanoma cell adhesion molecule gene (MCAM) to chromosome 11 band q23.3 by radiation hybrid mapping". Cytogenet. Cell Genet. 87 (3–4): 258. doi:10.1159/000015439. PMID 10702685.
^ Elshal MF, Khan SS, Takahashi Y, Solomon MA, McCoy JP (October 2005). "CD146 (Mel-CAM), an adhesion marker of endothelial cells, is a novel marker of lymphocyte subset activation in normal peripheral blood". Blood 106 (8): 2923–4. doi:10.1182/blood-2005-06-2307. PMID 16204154.
^ Elshal MF, Khan SS, Raghavachari N, Takahashi Y, Barb J, Bailey JJ, Munson PJ, Solomon MA, Danner RL, McCoy JP (2007). "A unique population of effector memory lymphocytes identified by CD146 having a distinct immunophenotypic and genomic profile". BMC Immunol. 8: 29. doi:10.1186/1471-2172-8-29. PMC 2248207. PMID 17999761.
^ Covas DT, Panepucci RA, Fontes AM, Silva WA, Orellana MD, Freitas MC, Neder L, Santos AR, Peres LC, Jamur MC, Zago MA (May 2008). "Multipotent mesenchymal stromal cells obtained from diverse human tissues share functional properties and gene-expression profile with CD146+ perivascular cells and fibroblasts". Exp. Hematol. 36 (5): 642–54. doi:10.1016/j.exphem.2007.12.015. PMID 18295964.
^ Russell KC, Phinney DG, Lacey MR, Barrilleaux BL, Meyertholen KE, O'Connor KC (April 2010). "In vitro high-capacity assay to quantify the clonal heterogeneity in trilineage potential of mesenchymal stem cells reveals a complex hierarchy of lineage commitment". Stem Cells 28 (4): 788–98. doi:10.1002/stem.312. PMID 20127798.

External links

CD146 Antigen at the US National Library of Medicine Medical Subject Headings (MeSH)

UpToDate Contents

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1. 妊娠性絨毛性疾患：病理 gestational trophoblastic disease pathology

English Journal

Different expression of CD146 in human normal and osteosarcoma cell lines.

Schiano C, Grimaldi V, Casamassimi A, Infante T, Esposito A, Giovane A, Napoli C.SourceDepartment of General Pathology, Chair of Clinical Pathology, Excellence Research Centre on Cardiovascular Disease, U.O.C. Immunohematology, Transfusion Medicine and Transplant Immunology [SIMT], Regional Reference Laboratory of Transplant Immunology [LIT], Azienda Universitaria Policlinico (AOU), 1st School of Medicine, Second University of Naples, Piazza Miraglia 2, 80138, Naples, Italy.
Medical oncology (Northwood, London, England).Med Oncol.2012 Dec;29(4):2998-3002. doi: 10.1007/s12032-012-0158-3. Epub 2012 Jan 21.
The CD146 cell membrane adhesion molecule is highly expressed on the cell surface of several tumours. The level of its expression has been found to correlate directly with tumour progression and metastatic potential, thus establishing CD146 as an important candidate of tumour growth and metastasis.
PMID 22271434

Levels of a subpopulation of platelets, but not circulating endothelial cells, predict early treatment failure in prostate cancer patients after prostatectomy.

Wong CK, Namdarian B, Chua J, Chin X, Speirs R, Nguyen T, Fankhauser M, Pedersen J, Costello AJ, Corcoran NM, Hovens CM.SourceAustralian Prostate Cancer Research Centre at Epworth Hospital, Richmond, Victoria, Australia.
British journal of cancer.Br J Cancer.2012 Oct 9. doi: 10.1038/bjc.2012.439. [Epub ahead of print]
Background:Angiogenesis is one of the hallmarks of cancer driving tumour growth and ultimately metastasis. Circulating endothelial cells (CECs) and circulating endothelial progenitor (CEPs) cells have been reported as candidate surrogate markers for tumour vascularisation. Our aim was to investigate
PMID 23047552

Japanese Journal

Tridimensional Response of human Dental Follicular Stem Cells onto a Synthetic Hydroxyapatite Scaffold

Mastrangelo Filiberto,Nargi Elena,Carone Luigi,Dolci Marco,Caciagli Francesco,Ciccarelli Renata,Anna De Lutiis Maria,Karapanou Virginia,Shaik Basha Y.,Conti Pio,Tete Stefano
Journal of health science 54(2), 154-161, 2008-04-01
… Populations of CD29+, CD90+, CD146+ and CD166+ were sorted by FAC sorter (FACS) analysis and were cultured in osteogenic medium and then, onto the scaffold. …
NAID 110006649665

Serum adiponectin and markers of endothelial injury in hemodialysis patients with arteriosclerosis obliterans

SAITO Osamu,SAITO Takako,OKUDA Kousuke,OKUDA Kenji,KOTODA Atsushi,AKIMOTO Tetsu,ANDO Yasuhiro,MUTO Shigeaki,ISHIKAWA San-e,KUSANO Eiji
Clinical and experimental nephrology 12(1), 58-64, 2008-02-01
NAID 10025351844

Related Pictures

★リンクテーブル★

リンク元	「ギセリン」
関連記事	「CD14」「CD1」

「ギセリン」

Melanoma cell adhesion molecule
Identifiers
Symbols	MCAM; CD146; MUC18
External IDs	OMIM: 155735 MGI: 1933966 HomoloGene: 4742 GeneCards: MCAM Gene
Gene Ontology
Molecular function	• protein binding;
Cellular component	• plasma membrane; • external side of plasma membrane; • integral to membrane;
Biological process	• glomerular filtration; • cell adhesion; • anatomical structure morphogenesis; • vascular wound healing;
	Sources: Amigo / QuickGO
Orthologs
	This box: view; talk; edit;

　　[★]

英: gicerin
同: 神経突起伸展因子受容体, NOF受容体, CD146, MCAM, MUC18, Mel-CAM

「CD14」

　　[★]

同？: リポ多糖受容体 LPS受容体 lipopolysaccharide receptor, LPS receptor

単球、マクロファージ、好中球の細胞膜上に存在し、LPS-LBP複合体をTLRを共役して受容し、TNF-α、IL-1を産生するシグナルを伝達するらしい

LPSの受容 (SMB.117)

LPSはマクロファージ、単球、顆粒球、Bリンパ球と反応し、多彩な生物活性を発現する。この反応には細胞表面のToll-like receptor。TLR-4がLPSのレセブタ-としてはたらき, LPSのシグナルを細胞内へと伝達する。このTLR-4の機能不全はLPSに対する応答性を失わせる。 TLR-4のほかに, CD14やMD-2と呼ばれる分子もLPSの認識に関与している。このTLRは分子群を形成し,ペプチドグリカン,リポタンパク質DNAなどの菌体成分を認識し,感染防御に重要なシグナルを伝達する。

発現細胞

骨髄性単球細胞

機能

LPSの直接の受容体であり、Toll-like receptorとともに細胞内へのシグナル伝達に関わる。
LPS-LBP複合体の受容体　←　日本語がよく分かりません

「CD1」

　　[★]

同: leucine-6, CD1A through E

種類

CD1a, CD1b, CD1c, CD1d

発現組織

胸腺皮質、ランゲルハンス細胞、樹状細胞、B細胞(CD1c)、腸上皮、平滑筋、血管上皮(CD1d)

分子量

43-49

機能

MHC class I-like molecule, associated with β2-microgloulin. Has specialized role in presentation of lipid antigens

[pmid10702685-1] Kuske MD, Johnson JP (1999). "Assignment of the human melanoma cell adhesion molecule gene (MCAM) to chromosome 11 band q23.3 by radiation hybrid mapping". Cytogenet. Cell Genet. 87 (3–4): 258. doi:10.1159/000015439. PMID 10702685.

[pmid16204154-2] Elshal MF, Khan SS, Takahashi Y, Solomon MA, McCoy JP (October 2005). "CD146 (Mel-CAM), an adhesion marker of endothelial cells, is a novel marker of lymphocyte subset activation in normal peripheral blood". Blood 106 (8): 2923–4. doi:10.1182/blood-2005-06-2307. PMID 16204154.

[pmid17999761-3] Elshal MF, Khan SS, Raghavachari N, Takahashi Y, Barb J, Bailey JJ, Munson PJ, Solomon MA, Danner RL, McCoy JP (2007). "A unique population of effector memory lymphocytes identified by CD146 having a distinct immunophenotypic and genomic profile". BMC Immunol. 8: 29. doi:10.1186/1471-2172-8-29. PMC 2248207. PMID 17999761.

[pmid18295964-4] Covas DT, Panepucci RA, Fontes AM, Silva WA, Orellana MD, Freitas MC, Neder L, Santos AR, Peres LC, Jamur MC, Zago MA (May 2008). "Multipotent mesenchymal stromal cells obtained from diverse human tissues share functional properties and gene-expression profile with CD146+ perivascular cells and fibroblasts". Exp. Hematol. 36 (5): 642–54. doi:10.1016/j.exphem.2007.12.015. PMID 18295964.

[pmid20127798-5] Russell KC, Phinney DG, Lacey MR, Barrilleaux BL, Meyertholen KE, O'Connor KC (April 2010). "In vitro high-capacity assay to quantify the clonal heterogeneity in trilineage potential of mesenchymal stem cells reveals a complex hierarchy of lineage commitment". Stem Cells 28 (4): 788–98. doi:10.1002/stem.312. PMID 20127798.

匿名

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案内

案内

CD146