ウイルス分離
WordNet
- (psychiatry) a defense mechanism in which memory of an unacceptable act or impulse is separated from the emotion originally associated with it
- a state of separation between persons or groups
- the act of isolating something; setting something apart from others (同)closing off
- a countrys withdrawal from international politics; "he opposed a policy of American isolation"
- a feeling of being disliked and alone
- relating to or caused by a virus; "viral infection"
PrepTutorEJDIC
- 孤立;隔離,分離
- ウィルスの;ウィルスが原因の
Wikipedia preview
出典(authority):フリー百科事典『ウィキペディア(Wikipedia)』「2017/09/20 07:59:11」(JST)
[Wiki en表示]
Viral culture is a laboratory test in which samples are placed with a cell type that the virus being tested for is able to infect. If the cells show changes, known as cytopathic effects, then the culture is positive.[1]
Traditional viral culture has been generally superseded by shell vial culture, in which the sample is centrifuged onto a single layer of cells and viral growth is measured by antigen detection methods. This greatly reduces the time to detection for slow growing viruses such as cytomegalovirus, for which the method was developed.[2] In addition, the centrifugation step in shell viral culture enhances the sensitivity of this method because after centrifugation, the viral particles of the sample are in close proximity to the cells.
Human and monkey cells are used in both traditional viral culture and shell vial culture.
Human virus types that can be identified by viral culture include adenovirus, cytomegalovirus, enteroviruses, herpes simplex virus, influenza virus, parainfluenza virus, rhinovirus, respiratory syncytial virus, varicella zoster virus, measles and mumps.[3] For these, the final identification method is generally by immunofluorescence, with exception of cytomegalovirus and rhinovirus, whose identification in a viral culture are determined by cytopathic effects.[3]
See also
- Cell culture
- Instruments used in microbiology
- Laboratory diagnosis of viral infections
- Viral plaque
References
- ^ Curtis, Jeanette; Caroline Rea (25 May 2007). "Viral culture". WebMD. Retrieved 2009-09-09.
- ^ Storch, Gregory A.; Bernard N. Fields; David Mahan Knipe; Peter M. Howley (2007). "Diagnostic virology". In David Mahan Knipe, Peter M. Howley. Fields' Virology. 1 (5th ed.). Lippincott Williams & Wilkins. p. 3177. ISBN 0-7817-6060-7.
- ^ a b Table 2 in: Leland DS, Ginocchio CC (January 2007). "Role of cell culture for virus detection in the age of technology". Clin. Microbiol. Rev. 20 (1): 49–78. PMC 1797634 . PMID 17223623. doi:10.1128/CMR.00002-06.
External links
- "Viral Culture, General". LabCorp. Retrieved 2009-09-09.
- "Growing viruses from clinical samples.". London Laboratory Services Group. Retrieved 2009-09-09.
Microbiology: Virus
|
Components |
- Viral envelope
- Capsid
- Viral protein
|
Viral life cycle |
- Viral entry
- Viral replication
- Viral shedding
- Virus latency
- Viroplasm
|
Genetics |
- Reassortment
- Antigenic shift
- Antigenic drift
- Phenotype mixing
|
By host |
- Bacteriophage
- Virophage
- Mycovirus
- Plant virus (Plant to Human)
- Animal virus
- Human virome
- Archea virus
- Amoeba virus
|
Other |
- Viral disease
- Helper virus
- Laboratory diagnosis of viral infections
- Viral load
- Virus-like particle
- Viral quantification
- Antiviral drug
- Neurotropic virus
- Oncovirus
- Social history of viruses
- Satellite virus
- Giant viruses
|
UpToDate Contents
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English Journal
- Progesterone-inducible cytokeratin 5-positive cells in luminal breast cancer exhibit progenitor properties.
- Axlund SD, Yoo BH, Rosen RB, Schaack J, Kabos P, Labarbera DV, Sartorius CA.SourceDepartment of Pathology, University of Colorado Denver, Anschutz Medical Center, Aurora, CO, USA.
- Hormones & cancer.Horm Cancer.2013 Feb;4(1):36-49. doi: 10.1007/s12672-012-0127-5. Epub 2012 Nov 27.
- Progestins play a deleterious role in the onset of breast cancer, yet their influence on existing breast cancer and tumor progression is not well understood. In luminal estrogen receptor (ER)- and progesterone receptor (PR)-positive breast cancer, progestins induce a fraction of cells to express cyt
- PMID 23184698
- Isolation and Characterization of Nucleotide-Binding Site Resistance Gene Homologues in Common Bean (Phaseolus vulgaris).
- Garzón LN, Oliveros OA, Rosen B, Ligarreto GA, Cook DR, Blair MW.AbstractABSTRACT Common bean production is constrained by many fungal, viral, and bacterial pathogens. Thus, the identification of resistance (R) genes is an important focal point of common bean research. The main goal of our study was to identify resistance gene homologues (RGH) in the crop, using degenerate primers designed from conserved sequences in the nucleotide-binding site (NBS) domains of R-genes from the model legume Medicago truncatula. Total DNA of the Andean common bean genotype G19833 was used for amplification of over 500 primer combinations. Sequencing of amplicons showed that 403 cloned fragments had uninterrupted open reading frames and were considered representative of functional RGH genes. The sequences were grouped at two levels of nucleotide identity (90 and 80%) and representative sequences of each group were used for phylogenetic analyses. The RGH sequence diversity of common bean was divided into TIR and non-TIR families, each with different clusters. The TIR sequences grouped into 14 clades while non-TIR sequences grouped into seven clades. Pairwise comparisons showed purifying selection, although some sequences may have been the result of diversifying selection. Knowledge about RGH genes in common bean can allow the design of molecular markers for pyramiding of resistance genes against various pathogens.
- Phytopathology.Phytopathology.2013 Feb;103(2):156-68. doi: 10.1094/PHYTO-07-12-0180-R.
- ABSTRACT Common bean production is constrained by many fungal, viral, and bacterial pathogens. Thus, the identification of resistance (R) genes is an important focal point of common bean research. The main goal of our study was to identify resistance gene homologues (RGH) in the crop, using degene
- PMID 23294404
Japanese Journal
- Epidemiological Survey of Severe Fever with Thrombocytopenia Syndrome Virus in Ticks in Nagasaki, Japan
- Survey on vertical infection of bovine viral diarrhea virus from fetal bovine sera in the field
- Epidemiological Survey of Severe Fever with Thrombocytopenia Syndrome Virus in Ticks in Nagasaki, Japan
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★リンクテーブル★
[★]
- 英
- viral isolation
- 関
- ウイルス
[★]
- 関
- virally、virogenic、virus
[★]