English Journal

Effects of canola proteins and hydrolysates on adipogenic differentiation of C3H10T/2 mesenchymal stem cells.

Alashi AM1, Blanchard CL2, Mailer RJ3, Agboola SO4, Mawson AJ4, Aluko RE5, Strappe P2.
Food chemistry.Food Chem.2015 Oct 15;185:226-32. doi: 10.1016/j.foodchem.2015.03.054. Epub 2015 Mar 27.
This study assessed the ability of canola protein isolate (CPI) and enzymatic hydrolysates (CPHs) to inhibit adipogenic differentiation of C3H10T1/2 murine mesenchymal stem cells in vitro. Cell viability was maintained at concentrations of 60 μg/ml of sample. Cells treated with Alcalase hydrolysate
PMID 25952862

Buprenorphine detection in urine using liquid chromatography-high-resolution mass spectrometry: comparison with cloned enzyme donor immunoassay (ThermoFisher) and homogeneous enzyme immunoassay (immunalysis).

Belsey SL1, Couchman L2, Flanagan RJ2.
Journal of analytical toxicology.J Anal Toxicol.2014 Sep;38(7):438-43. doi: 10.1093/jat/bku060. Epub 2014 Jun 12.
A sensitive liquid chromatographic-high-resolution mass spectrometric (LC-HR-MS) assay for buprenorphine and its urinary metabolites has been developed that requires minimal sample preparation. The results obtained have been compared with those given by (i) cloned enzyme donor immunoassay (CEDIA) an
PMID 24925983

A comparative rapid and sensitive method to screen l-asparaginase producing fungi.

Dhale MA1, Mohan-Kumari HP2.
Journal of microbiological methods.J Microbiol Methods.2014 Jul;102:66-8. doi: 10.1016/j.mimet.2014.04.010. Epub 2014 Apr 30.
Fungi are well known to produce various industrial enzymes and secondary metabolites with different colours. Fungi producing l-asparaginase enzyme are conventionally screened on medium containing phenol red (PR). The contrast between enzyme-hydrolysed zone and unhydrolysed l-asparagine is not very e
PMID 24794733