Hydrolytic cationic ester microparticles for highly efficient DNA vaccine delivery.
Zhang L, Sinclair A, Cao Z, Ella-Menye JR, Xu X, Carr LR, Pun SH, Jiang S.SourceDepartment of Chemical Engineering, University of Washington Seattle, WA 98195, USA.
Small (Weinheim an der Bergstrasse, Germany).Small.2013 Oct 25;9(20):3439-44. doi: 10.1002/smll.201202727. Epub 2013 May 10.
DNA vaccination holds great potential to be a safer and more efficient alternative to traditional vaccination strategies, but the current lack of nontoxic and effective delivery systems is the greatest impediment to its clinical implementation. In this work, a convenient one-step method is used to p
Determination of the GH3.12 protein conformation through HPLC-integrated SAXS measurements combined with X-ray crystallography.
Round A, Brown E, Marcellin R, Kapp U, Westfall CS, Jez JM, Zubieta C.SourceEuropean Molecular Biology Laboratory, Grenoble Outstation, 6 Rue Jules Horowitz, 38042 Grenoble, France.
The combination of protein crystallography and small-angle X-ray scattering (SAXS) provides a powerful method to investigate changes in protein conformation. These complementary structural techniques were used to probe the solution structure of the apo and the ligand-bound forms of the Arabidopsis t
Expression, purification, crystallization and preliminary crystallographic study of FtsA from methicillin-resistant Staphylococcus aureus.
Fujita J, Miyazaki Y, Hirose M, Nagao C, Mizohata E, Matsumoto Y, Mizuguchi K, Inoue T, Matsumura H.SourceDepartment of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
FtsA from methicillin-resistant Staphylococcus aureus (MRSA) was cloned, overexpressed and purified. The protein was crystallized using the sitting-drop vapour-diffusion technique. A cocrystal with β-γ-imidoadenosine 5'-phosphate (AMPPNP; a nonhydrolysable ATP analogue) was grown using PEG 3350 as
This International Standard specifies a method for the determination, in detergents, of anionic-active matter hydrolyzable and non-hydrolyzable under acid conditions. This active matter includes alkyl sulfates and hydroxysulfates and ...