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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2015/05/15 10:47:49」(JST)
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English Journal
- A unique N-terminal Sequence in the Carnation Italian Ringspot Virus p36 Replicase-Associated Protein Interacts with the Host-Cell ESCRT-I Component Vps23.
- Richardson LG1, Clendening EA, Sheen H, Gidda SK, White KA, Mullen RT.Author information 1Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada.AbstractLike most positive-strand RNA viruses, infection by plant tombusviruses results in extensive rearrangement of specific host-cell organelle membranes that serve as the sites of viral replication. The tombusvirus Tomato bushy stunt virus (TBSV) replicates within spherules derived from the peroxisomal boundary membrane, a process that involves the coordinated action of various viral and cellular factors, including constituents of the endosomal sorting complex required for transport (ESCRT). ESCRT is comprised of a series of protein sub-complexes (i.e., ESCRT-0 -I, -II and -III) that normally participate in late endosome biogenesis and some of which are also hijacked by certain enveloped retroviruses (e.g., HIV) for viral budding from the plasma membrane. Here we show that the replication of Carnation Italian ringspot virus (CIRV), a tombusvirus that replicates at mitochondrial membranes also relies on ESCRT. In plant cells, CIRV recruits the ESCRT-I protein, Vps23, to mitochondria through an interaction that involves a unique region in the N terminus of the p36 replicase-associated protein that is not conserved in TBSV or other peroxisome-targeted tombusviruses. The interaction between p36 and Vps23 also involves the Vps23 C-terminal steadiness box domain and not its N-terminal ubiquitin E2 variant domain, which in the case of TBSV (and enveloped retroviruses) mediates the interaction with ESCRT. Overall, these results provide evidence that CIRV uses a unique N-terminal sequence for the recruitment of Vps23 that is distinct from those used by TBSV and certain mammalian viruses for ESCRT recruitment. Characterization of this novel interaction with Vps23 contributes to our understanding of how CIRV may have evolved to exploit key differences in the plant ESCRT machinery.
- Journal of virology.J Virol.2014 Mar 26. [Epub ahead of print]
- Like most positive-strand RNA viruses, infection by plant tombusviruses results in extensive rearrangement of specific host-cell organelle membranes that serve as the sites of viral replication. The tombusvirus Tomato bushy stunt virus (TBSV) replicates within spherules derived from the peroxisomal
- PMID 24672030
- Dazzling history.
- Sumner T.
- Science (New York, N.Y.).Science.2014 Mar 7;343(6175):1092-3. doi: 10.1126/science.343.6175.1092.
- PMID 24604191
- A template role of double-stranded RNA in tombusvirus replication.
- Kovalev N1, Pogany J, Nagy PD.Author information 1Department of Plant Pathology, University of Kentucky, Lexington, KY 40546.AbstractReplication of plus-strand RNA viruses of plants is a relatively simple process that involves complementary (-)RNA synthesis and subsequent (+)RNA synthesis. However, the actual replicative form of the (-)RNA template in case of plant (+)RNA viruses is not yet established unambiguously. In this paper, using a cell-free replication assay supporting full cycle of viral replication, we show that replication of Tomato bushy stunt virus (TBSV) leads to the formation of double-stranded (ds)RNA. Using ribonuclease digestion, DNAzyme, and RNA mobility-shift assays, we demonstrate the absence of naked (-)RNA templates during replication. Time course experiments showed the rapid appearance of dsRNA earlier than the bulk production of new (+)RNAs, suggesting an active role for dsRNA in replication. Radioactive nucleotide chase experiments showed that the mechanism of TBSV replication involves the use of dsRNA templates in strand displacement reaction, where the newly synthesized (+)strand replaces the original (+)strand RNA in the dsRNA. We propose that the use of dsRNA as a template for (+)RNA synthesis by the viral replicase is facilitated by recruited host DEAD-box helicases and the viral p33 RNA chaperone protein. Altogether, this replication strategy allows TBSV to separate (-) and (+)-strand synthesis in time and regulate asymmetrical RNA replication that leads to abundant (+)RNA progeny.
- Journal of virology.J Virol.2014 Mar 5. [Epub ahead of print]
- Replication of plus-strand RNA viruses of plants is a relatively simple process that involves complementary (-)RNA synthesis and subsequent (+)RNA synthesis. However, the actual replicative form of the (-)RNA template in case of plant (+)RNA viruses is not yet established unambiguously. In this pape
- PMID 24600009
Japanese Journal
- 新潟県下のトルコギキョウにおけるLisianthus necrotic stunt virusの発生
- 伊藤 由衣,小田 正之,棚橋 恵,佐野 義孝,Ito Yui,Oda Masayuki,Tanahashi Megumu,Sano Yoshitaka
- 新潟大学農学部研究報告 64(1), 37-41, 2011-09
- … Sequence identity at the nucleotide level was 99% with Nag-4 isolate of Lisianthus necrotic stunt virus (LiNSV),a novel tombusvirus recently found in Nagano prefecture. …
- NAID 120005239931
- 新潟県下のトルコギキョウにおけるLisianthus necrotic stunt virusの発生
- 伊藤 由衣,小田 正之,棚橋 恵 [他],佐野 義孝
- 新潟大学農学部研究報告 64(1), 37-41, 2011-09
- 2009年10月、新潟県のトルコギキョウ（Eustoma grangiflorum）にえそ萎縮症状を引き起こすウイルス病が発生した。ウイルス（Nig-09株）感染葉からトータルRNAを抽出し、各種トンブスウイルスの外被タンパク質（CP）遺伝子の全域を増幅できるプライマーを用いたRT-PCRにより、CP領域を含むおよそ1300bpのcDNAが増幅された。塩基配列を解析し、CP領域のアミノ酸配列を他の …
- NAID 120005068696
- トルコギキョウに発生する2種トンブスウイルスのRT-PCR法による識別検出
- 伊山 幸秀,守川 俊幸,島 嘉輝 [他]
- 北陸病害虫研究会報 (58), 19-23, 2009-12
- NAID 40017017388
Related Links
- Year of event Event Reference 1935 First report of a tombusvirus Smith KM (1935) Ann Appl Biol 22:731–741 1978 Crystal structure of TBSV solved Harrison SC et al (1978) Nature 276:368–373 1987 First report of a plant virus ...
- A knowledge resource to understand virus diversity ... VIRION Non-enveloped, spherical, icosahedral, about 28-34 nm in diameter, capsid with a T=3 icosahedral symmetry. 30 hexameric capsomers per nucleocapsid (virion ...
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★リンクテーブル★
| リンク元 | 「トンブスウイルス」「Tombusviridae」「tomato bushy stunt virus」「dianthovirus」 |
「トンブスウイルス」
「Tombusviridae」
- ンブスウイルス科