単純タンパク質

WordNet

unornamented; "a simple country schoolhouse"; "her black dress--simple to austerity"
any herbaceous plant having medicinal properties
(botany) of leaf shapes; of leaves having no divisions or subdivisions (同)unsubdivided
having few parts; not complex or complicated or involved; "a simple problem"; "simple mechanisms"; "a simple design"; "a simple substance"
any of a large group of nitrogenous organic compounds that are essential constituents of living cells; consist of polymers of amino acids; essential in the diet of animals for growth and for repair of tissues; can be obtained from meat and eggs and milk and legumes; "a diet high in protein"

PrepTutorEJDIC

『簡単な』容易な,分かりやすい / (複合に対して)単一の / 『単純な』,込み入っていない / 『純然たる』,全くの / 『飾り気のない』,簡素な,地味な,質素な / 『もったいぶらない』;誠実な,実直な / お人よしの,だまされやすい / 《文》地位のない,普通の,平(ひら)の
薬草,薬用植物
蛋白(たんばく)質

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1. 維持透析患者における蛋白質摂取 protein intake in maintenance hemodialysis patients
2. プロテインＳ欠乏症 protein s deficiency
3. プロテインＣ欠乏症 protein c deficiency
4. Factor V Leiden and activated protein C resistance
5. Hypertrophic cardiomyopathy: Gene mutations and clinical genetic testing

English Journal

Gold-nanobeacons for gene therapy: evaluation of genotoxicity, cell toxicity and proteome profiling analysis.

Conde J, Larguinho M, Cordeiro A, Raposo LR, Costa PM, Santos S, Diniz MS, Fernandes AR, Baptista PV.Author information CIGMH, DCV, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa , Caparica , Portugal.AbstractAbstract Antisense therapy is a powerful tool for post-transcriptional gene silencing suitable for down-regulating target genes associated to disease. Gold nanoparticles have been described as effective intracellular delivery vehicles for antisense oligonucleotides providing increased protection against nucleases and targeting capability via simple surface modification. We constructed an antisense gold-nanobeacon consisting of a stem-looped oligonucleotide double-labelled with 3'-Cy3 and 5'-Thiol-C6 and tested for the effective blocking of gene expression in colorectal cancer cells. Due to the beacon conformation, gene silencing was directly detected as fluorescence increases with hybridisation to target, which can be used to assess the level of silencing. Moreover, this system was extensively evaluated for the genotoxic, cytotoxic and proteomic effects of gold-nanobeacon exposure to cancer cells. The exposure was evaluated by two-dimensional protein electrophoresis followed by mass spectrometry to perform a proteomic profile and 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay, glutathione-S-transferase assay, micronucleus test and comet assay to assess the genotoxicity. This integrated toxicology evaluation showed that the proposed nanotheranostics strategy does not exhibit significant toxicity, which is extremely relevant when translating into in vivo systems.
Nanotoxicology.Nanotoxicology.2014 Aug;8:521-32. doi: 10.3109/17435390.2013.802821. Epub 2013 May 28.
Abstract Antisense therapy is a powerful tool for post-transcriptional gene silencing suitable for down-regulating target genes associated to disease. Gold nanoparticles have been described as effective intracellular delivery vehicles for antisense oligonucleotides providing increased protection aga
PMID 23642008

Use of principal component analysis for differentiation of gelatine sources based on polypeptide molecular weights.

Nur Azira T1, Che Man YB1, Raja Mohd Hafidz RN1, Aina MA1, Amin I2.Author information 1Laboratory of Halal Science Research, Halal Products Research Institute, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.2Laboratory of Halal Science Research, Halal Products Research Institute, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. Electronic address: aminis@upm.edu.my.AbstractThe study was aimed to differentiate between porcine and bovine gelatines in adulterated samples by utilising sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) combined with principal component analysis (PCA). The distinct polypeptide patterns of 6 porcine type A and 6 bovine type B gelatines at molecular weight ranged from 50 to 220kDa were studied. Experimental samples of raw gelatine were prepared by adding porcine gelatine in a proportion ranging from 5% to 50% (v/v) to bovine gelatine and vice versa. The method used was able to detect 5% porcine gelatine added to the bovine gelatine. There were no differences in the electrophoretic profiles of the jelly samples when the proteins were extracted with an acetone precipitation method. The simple approach employing SDS-PAGE and PCA reported in this paper may provide a useful tool for food authenticity issues concerning gelatine.
Food chemistry.Food Chem.2014 May 15;151:286-92. doi: 10.1016/j.foodchem.2013.11.066. Epub 2013 Nov 20.
The study was aimed to differentiate between porcine and bovine gelatines in adulterated samples by utilising sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) combined with principal component analysis (PCA). The distinct polypeptide patterns of 6 porcine type A and 6 bovine typ
PMID 24423534

Development and validation of a lateral flow assay for the detection of crustacean protein in processed foods.

Koizumi D1, Shirota K2, Akita R2, Oda H2, Akiyama H3.Author information 1Central Research Institute, Maruha Nichiro Holdings, Inc., 16-2 Wadai, Tsukuba, Ibaraki 300-4295, Japan. Electronic address: d-koizumi@maruha-nichiro.co.jp.2Central Research Institute, Maruha Nichiro Holdings, Inc., 16-2 Wadai, Tsukuba, Ibaraki 300-4295, Japan.3National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.AbstractWe developed and validated a novel lateral flow assay for the detection of crustacean protein in processed foods. This assay had high sensitivity; the visual detection limit for shrimp protein extract was 25μg/L, equivalent to 1μg/g protein in a food sample, and results could be obtained within 20min without sophisticated procedures or expensive equipment. Concordance between our assay and another validated quantitative enzyme-linked immunosorbent assay was 97% for commercially processed foods. This assay is rapid, simple, reliable, and highly correlated with validated enzyme-linked immunosorbent assays and is thus suitable for monitoring of food products, especially in food-processing facilities.
Food chemistry.Food Chem.2014 May 1;150:348-52. doi: 10.1016/j.foodchem.2013.10.130. Epub 2013 Nov 4.
We developed and validated a novel lateral flow assay for the detection of crustacean protein in processed foods. This assay had high sensitivity; the visual detection limit for shrimp protein extract was 25μg/L, equivalent to 1μg/g protein in a food sample, and results could be obtained within 20
PMID 24360461

Japanese Journal

Development of an improved method for quantitative analysis of skin blotting: Increasing reliability and applicability for skin assessment

Ogai Kazuhiro,Matsumoto Masaru,Minematsu T.,Kitamura K.,Kobayashi M. ,Sugama Junko,Sanada H.
International Journal of Cosmetic Science 37(4), 425-432, 2015-08-01
… Methods: To normalize individual differences, we utilized a total protein as a 'normalizer' with calibration curves. … For evaluation, we performed a simple simulation experiment, in which the same concentration of a protein of interest [tumour necrosis factor (TNF)-α] was applied at different volumes as a virtual individual difference. …
NAID 120005593971

Nanoconstriction-based electrodeless dielectrophoresis chip for nanoparticle and protein preconcentration

Chiou Chi-Han,Chien Liang-Ju,Kuo Ju-Nan
Appl. Phys. Express 8(8), 085201, 2015-07-15
… Notably, the nanoconstriction is fabricated using a simple thermal-oxidation shrinkage process. … Thus, the proposed EDEP chip has significant potential for achieving rapid and highly sensitive protein detection and for biomarker discovery applications. …
NAID 150000111046

特定原材料検査における乾燥海苔製品中のえび・かにDNA検出法の検討

橋本博之,本郷猛,林千恵子 [他],中村和宏,中西希代子,池田惠,安達玲子,穐山浩,手島玲子,矢野竹男
日本食品化学学会誌 22(1), 1-10, 2015-04-24
甲殻類タンパク質(トロポミオシン)は、海苔を含む加工食品で高頻度に検出されている。アレルギー物質の表示に関する日本の規制では、抽出されたエビとカニのDNAを検出するためのPCR法が、加工食品中のエビとカニを区別するために規定されている。海苔を含有した加工食品では、エビとカニのDNAを抽出することが難しい。我々は、DNeasy mericon Food kitのDNA抽出プロトコールを改良し、凍結乾 …
NAID 110009934911