English Journal

α-2,3-sialyltransferase expression level impacts the kinetics of lipooligosaccharide sialylation, complement resistance, and the ability of Neisseria gonorrhoeae to colonize the murine genital tract.

Lewis LA1, Gulati S2, Burrowes E2, Zheng B2, Ram S2, Rice PA2.
mBio.MBio.2015 Feb 3;6(1). pii: e02465-14. doi: 10.1128/mBio.02465-14.
Neisseria meningitidis and Neisseria gonorrhoeae modify the terminal lacto-N-neotetraose moiety of their lipooligosaccharide (LOS) with sialic acid. N. gonorrhoeae LOS sialylation blocks killing by complement, which is mediated at least in part by enhanced binding of the complement inhibitor facto
PMID 25650401

N-acetylgalactosaminide α2,6-sialyltransferase II is a candidate enzyme for sialylation of galactose-deficient IgA1, the key autoantigen in IgA nephropathy.

Stuchlova Horynova M1, Vrablikova A1, Stewart TJ2, Takahashi K3, Czernekova L1, Yamada K4, Suzuki H4, Julian BA5, Renfrow MB6, Novak J2, Raska M1.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association.Nephrol Dial Transplant.2015 Feb;30(2):234-8. doi: 10.1093/ndt/gfu308. Epub 2014 Oct 3.
BACKGROUND: Galactose-deficient O-glycans in the hinge region (HR) of immunoglobulin A1 (IgA1) play a key role in the pathogenesis of IgA nephropathy (IgAN). O-Glycans of circulatory IgA1 consist of N-acetylgalactosamine (GalNAc) with a β1,3-linked galactose; both sugars may be sialylated. In patie
PMID 25281698

Galactosyl-lactose sialylation using Trypanosoma cruzi trans-sialidase as the biocatalyst and bovine κ-casein-derived glycomacropeptide as the donor substrate.

Wilbrink MH1, ten Kate GA1, van Leeuwen SS1, Sanders P1, Sallomons E2, Hage JA3, Dijkhuizen L4, Kamerling JP1.
Applied and environmental microbiology.Appl Environ Microbiol.2014 Oct;80(19):5984-91. doi: 10.1128/AEM.01465-14. Epub 2014 Jul 25.
trans-Sialidase (TS) enzymes catalyze the transfer of sialyl (Sia) residues from Sia(α2-3)Gal(β1-x)-glycans (sialo-glycans) to Gal(β1-x)-glycans (asialo-glycans). Aiming to apply this concept for the sialylation of linear and branched (Gal)nGlc oligosaccharide mixtures (GOS) using bovine κ-casei
PMID 25063655