- エキソヌクレアーゼ遺伝子。rec Aが関与する経路とは別の組み換え経路に関与する。rec Jとsbc Cの両方に変異をもつことにより、Z-DNAの再構成を抑制し、DNAの安定性を向上する。recJとrecBに両方変異をもつことにより、rec A変異株と同様の表現型を示す。SURE® やSURE® 2株はrecB、recJ、sbcC遺伝子を欠損している。(参考1)
参考
- http://www.chem-agilent.com/stratagene/strategies/pdfs/19.2/Strategies%20Vol.2%20No.2_p7.pdf
WordNet
- the syllable naming the second (supertonic) note of any major scale in solmization (同)ray
PrepTutorEJDIC
- レ(全音階の第2音)
- =recreation
English Journal
- RecF recombination pathway in Escherichia coli cells lacking RecQ, UvrD and HelD helicases.
- Buljubašić M, Repar J, Zahradka K, Dermić D, Zahradka D.SourceDivision of Molecular Biology, Ruđer Bošković Institute, Bijenička 54, 10000 Zagreb, Croatia.
- DNA repair.DNA Repair (Amst).2012 Feb 16. [Epub ahead of print]
- In recBCD sbcB sbcC(D) mutants of Escherichia coli homologous recombination proceeds via RecF pathway, which is thought to require RecQ, UvrD and HelD helicases at its initial stage. It was previously suggested that depletion of all three helicases totally abolishes the RecF pathway. The present stu
- PMID 22342069
- The archaeal CMG (CDC45/RecJ, MCM, GINS) complex is a conserved component of the DNA replication system in all archaea and eukaryotes.
- Makarova KS, Koonin EV, Kelman Z.AbstractABSTRACT:
- Biology direct.Biol Direct.2012 Feb 13;7(1):7. [Epub ahead of print]
- ABSTRACT:BACKGROUND: In eukaryotes, the CMG (CDC45, MCM, GINS) complex containing the replicative helicase MCM is a key player in DNA replication. Archaeal homologs of the eukaryotic MCM and GINS proteins have been identified but until recently no homolog of the CDC45 protein was known. Two recent
- PMID 22329974
- Involvement of pnp in survival to UV radiation in Escherichia coli K12.
- Rath D, Mangoli SH, Pagedar AR, Jawali N.SourceMolecular Biology Division, Bhabha Atomic Research Centre, Trombay, Mumbai-400 085, India.
- Microbiology (Reading, England).Microbiology.2012 Feb 9. [Epub ahead of print]
- Polynucleotide phosphorylase (PNPase), a multifunctional protein, is a 3',5' exoribonuclease or exodeoxyribonuclease in the presence of inorganic phosphate-(Pi) and extend a 3'-OH of RNA or ssDNA in the presence of ADP or dADP. In Escherichia coli PNPase is known to protect against H2O2- and MMC-ind
- PMID 22322961
Japanese Journal
- The Roles of RecJ and RecQ in Formation of Deletion Mutation in Escherichia coli
- DNAのエンドジョイニングの分子機構:2種類の非相同的組換え : 第28回大会シンポジウム : II.新しい変異原検出法へのアプローチ
- 環境のストレスによって誘導される非相同的組換え : DNAの切断と再結合の分子機構
- RecQ and RecJ process blocked replication forks prior to the resumption of replication in UV-irradiated Escherichia coli
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Related Pictures
★リンクテーブル★
[★]
- 関
- 大腸菌
- e14– (McrA–) △(mcrCB-hsdSMR-mrr)171 endA1 supE44 thi-1 gyrA96 relA1 lac recB recJ sbcC umuC::Tn5 (Kanr) uvrC [F'proAB lacIqZ△M15 Tn10 (Tetr)]
特徴
- 不安定なDNAのクローニングでも成功に導くコンピテントセル
- 逆位リピートやZ-DNAを含むDNAの安定性を向上
- 高品質ミニプレップDNAを生産
- F' エピソームを含み、一本鎖DNA レスキューや青/白スクリーニングが可能
参考
- http://www.chem-agilent.com/contents.php?id=300473
[★]
- エキソヌクレアーゼI遺伝子。DNAの組み換えに関与する。recJとsbcCの両方に変異をもつことにより、Z-DNAの再構成を抑制する。SURE® やSURE® 2株はこの遺伝子を欠損している。(参考1)
参考
- http://www.chem-agilent.com/stratagene/strategies/pdfs/19.2/Strategies%20Vol.2%20No.2_p7.pdf