Wikipedia preview
出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2016/09/13 17:46:45」(JST)
wiki en
UpToDate Contents
全文を閲覧するには購読必要です。 To read the full text you will need to subscribe.
- 1. HIV血清陰性患者におけるクリプトコッカス髄膜脳炎の臨床症状および診断 clinical manifestations and diagnosis of cryptococcal meningoencephalitis in hiv seronegative patients
- 2. 色素内視鏡検査 chromoendoscopy
- 3. 中枢神経系以外のクリプトコッカス感染症 cryptococcal infection outside the central nervous system
- 4. 原発性線毛機能不全（線毛不動症候群） primary ciliary dyskinesia immotile cilia syndrome
- 5. プリオンの生物学および遺伝学 biology and genetics of prions
English Journal
- Pleural mesothelium lubrication after phospholipase treatment.
- Bodega F1, Sironi C1, Porta C1, Zocchi L1, Agostoni E2.Author information 1Dipartimento di Fisiopatologia e dei Trapianti, Sezione di Fisiologia Umana, Università degli Studi di Milano, Milan, Italy.2Dipartimento di Fisiopatologia e dei Trapianti, Sezione di Fisiologia Umana, Università degli Studi di Milano, Milan, Italy. Electronic address: emilio.agostoni@unimi.it.AbstractCoefficient of kinetic friction (μ) of rabbit pleural mesothelium increased after short treatment of specimens with phospholipase C. This increase was removed by addition of a solution with hyaluronan or sialomucin, as previously shown in post-blotting Ringer or after short pronase treatment. After phospholipase μ decreased with increase in sliding velocity, but at highest velocity it was still greater than control; this difference was removed by addition of hyaluronan or sialomucin, as in post-blotting Ringer or after short pronase treatment. Hyaluronan placed on specimen before phospholipase treatment reduced increase in μ by protecting phospholipids from enzyme, as shown by others for alveolar and synovial phospholipids. Samples of parietal pleura stained with silver nitrate showed that mesothelial cells were not disrupted by short phospholipase treatment. Instead, they were disrupted if this treatment was preceded by a short pronase treatment; but even after this disruption addition of hyaluronan or sialomucin brought μ back to control.
- Respiratory physiology & neurobiology.Respir Physiol Neurobiol.2014 Apr 1;194:49-53. doi: 10.1016/j.resp.2014.01.016. Epub 2014 Jan 28.
- Coefficient of kinetic friction (μ) of rabbit pleural mesothelium increased after short treatment of specimens with phospholipase C. This increase was removed by addition of a solution with hyaluronan or sialomucin, as previously shown in post-blotting Ringer or after short pronase treatment. After
- PMID 24486606
- Zebrafish (Danio rerio) as a possible bioindicator of epigenetic factors present in drinking water that may affect reproductive function: is chorion an issue?
- Martinez-Sales M, García-Ximénez F, Espinós F.Author information Aquaculture and Environmental Research Group (ACUMA), Universidad Politécnica de Valencia, Camino de Vera 14, 46022, Valencia, Spain.AbstractSummary Emerging organic contaminants have been monitored in stream waters, raw and finished waters and wastewater effluents. Most of these contaminants, such as epigenetic substances, have been detected at very low levels. Unfortunately, their complete monitoring and/or removal are very difficult, given the increasing presence of new contaminants and due to analytical and economic considerations. For this reason, bioindicators are used as an alternative to monitor their presence. To this end, zebrafish is being used to assess certain contaminants in water quality studies. As our long-term aim is to determine if zebrafish (Danio rerio) can be used to detect environmental epigenetic factors in drinking waters with effects on human reproduction, an initial question is whether the chorion could interfere with the possible action of epigenetic factors in two reproductive events: genital ridge formation and migration of the primordial germ cells (PGCs) to these genital ridges. In the first experiment, we attempted to partially degrade the chorion of mid blastula transition (MBT) embryos with pronase, with acceptable survival rates at 5 days post fertilisation (dpf), with the group exposed for 15 min giving the best survival results. As denuded early embryos require a specific culture medium, in the next experiment embryo survival was evaluated when they were cultured up to 5 dpf in drinking waters from six different sources. Results showed a negative effect on embryo survival at 5 dpf from several waters but not in others, thus distorting the survival outcomes. These results suggest using embryos with the chorion intact from the outset when drinking waters from different sources are to be tested.
- Zygote (Cambridge, England).Zygote.2014 Mar 5:1-6. [Epub ahead of print]
- Summary Emerging organic contaminants have been monitored in stream waters, raw and finished waters and wastewater effluents. Most of these contaminants, such as epigenetic substances, have been detected at very low levels. Unfortunately, their complete monitoring and/or removal are very difficult,
- PMID 24598355
- Binding of Clostridium botulinum C3 exoenzyme to intact cells.
- Rohrbeck A1, von Elsner L, Hagemann S, Just I.Author information 1Institute of Toxicology, Hannover Medical School, Carl-Neuberg-Str. 1, D-30625, Hannover, Germany, rohrbeck.astrid@mh-hannover.de.AbstractC3 from Clostridium botulinum (C3) specifically modifies Rho GTPases RhoA, RhoB, and RhoC by mono-ADP-ribosylation. The confined substrate profile of C3 is the basis for its use as pharmacological tool in cell biology to study cellular functions of Rho GTPases. Although C3 exoenzyme does not possess a cell-binding/-translocation domain, C3 is taken up by intact cells via an unknown mechanism. In the present work, binding of C3 to the hippocampus-derived HT22 cells and J774A.1 macrophages was characterized. C3 bound concentration-dependent to HT22 and J774A.1 cells. Pronase treatment of intact cells significantly reduced both C3 binding and C3 cell entry. Removal of sugar residues by glycosidase F treatment resulted in an increased binding of C3, but a reduced cell entry. To explore the involvement of phosphorylation in the binding process of C3, intact HT22 and J774A.1 cells were pre-treated with vanadate prior to incubation with C3. Inhibition of de-phosphorylation by vanadate resulted in an increased binding of C3. To differentiate between intracellular and extracellular phosphorylation, intact cells were treated with CIP (calf intestine phosphatase) to remove extracellular phosphate residues. The removal of phosphate residues resulted in a strong reduction in binding of C3 to cells. In sum, the C3 membranous binding partner is proteinaceous, and the glycosylation as well as the phosphorylation state is critical for efficient binding of C3.
- Naunyn-Schmiedeberg's archives of pharmacology.Naunyn Schmiedebergs Arch Pharmacol.2014 Mar 2. [Epub ahead of print]
- C3 from Clostridium botulinum (C3) specifically modifies Rho GTPases RhoA, RhoB, and RhoC by mono-ADP-ribosylation. The confined substrate profile of C3 is the basis for its use as pharmacological tool in cell biology to study cellular functions of Rho GTPases. Although C3 exoenzyme does not possess
- PMID 24584821
Japanese Journal
- 腸管洗浄液の胃内洗浄効果に関する臨床研究
- Progress of Digestive Endoscopy 86(1), 49-52, 2015
- NAID 130005084337
- プロナーゼを用いた透明帯菲薄化処理が牛胚の発育及び受胎率に及ぼす影響
- 日本獣医師会雑誌 67(11), 833-838, 2014
- NAID 130004888047
- Evaluation of Zona Pellucida Function for Sperm Penetration During In Vitro Fertilization in Pigs
- The Journal of reproduction and development 59(4), 385-392, 2013-08-01
- NAID 10031195876
Related Links
- Pronase 抽出源： Streptomyces griseus ヌクレアーゼフリー 凍結乾燥，非滅菌 製品番号 梱包単位 希望価格 国内在庫 有効期限 保管温度 毒劇 10165921001 1 g ￥12,500 国内在庫 11-20 現出荷品有効期限 2015-03-31 冷蔵 (2-8 ) ...
- PRONASE MS 薬価基準収載年月 1995年5月 販売開始年月 1995年6月 貯法・使用期限等 貯法 室温保存、気密容器 使用期限 外箱に表示 注意 取扱い上の注意の項参照 規制区分 **処方せん医薬品 （注意－医師等の処方せんにより ...
Related Pictures
★リンクテーブル★
| リンク元 | 「プロナーゼ」 |
「プロナーゼ」
- タンパク分解酵素の混合物