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English Journal

Formation of the N(2)-acetyl-2,6-diaminopurine oligonucleotide impurity caused by acetyl capping.

Rodriguez AA1, Cedillo I2, Mowery BP2, Gaus HJ2, Krishnamoorthy SS2, McPherson AK2.
Bioorganic & medicinal chemistry letters.Bioorg Med Chem Lett.2014 Aug 1;24(15):3243-6. doi: 10.1016/j.bmcl.2014.06.025. Epub 2014 Jun 18.
The acetyl 'capping' reaction routinely employed during phosphorothioate oligonucleotide synthesis has been implicated in the formation of an impurity species with a mass 41amu greater than the expected oligonucleotide molecule. The impurity has been found to arise by conversion of a protected guani
PMID 24980055

Importance of rigorous in vitro evaluation of prospective cell binding aptamers.

Avci-Adali M1, Mludek K, Perle N, Stoll H, Schlensak C, Wendel HP.
Nucleic acid therapeutics.Nucleic Acid Ther.2014 Aug;24(4):250-7. doi: 10.1089/nat.2014.0487.
Hitherto, several aptamers have been selected against cell surface molecules. The use of these aptamers for in vivo applications requires the prior in-depth in vitro evaluation of cell specific binding. Here, we demonstrate the in vitro tests, which are imperatively necessary to evaluate aptamers pr
PMID 25054517

TCP1 complex proteins interact with phosphorothioate oligonucleotides and can co-localize in oligonucleotide-induced nuclear bodies in mammalian cells.

Liang XH1, Shen W2, Sun H2, Prakash TP3, Crooke ST2.
Nucleic acids research.Nucleic Acids Res.2014 Aug 1;42(12):7819-32. doi: 10.1093/nar/gku484. Epub 2014 May 26.
Phosphorothioate (PS) antisense oligonucleotides (ASOs) have been successfully developed as drugs to reduce the expression of disease-causing genes. PS-ASOs can be designed to induce degradation of complementary RNAs via the RNase H pathway and much is understood about that process. However, interac
PMID 24861627

The systemic administration of an anti-miRNA oligonucleotide encapsulated pH-sensitive liposome results in reduced level of hepatic microRNA-122 in mice

Hatakeyama Hiroto,Murata Manami,Sato Yusuke,Takahashi Mayumi,Minakawa Noriaki,Matsuda Akira,Harashima Hideyoshi
Journal of controlled release 173, 43-50, 2014-01-10
… Anti-microRNA oligonucleotides including 2'-OMe and phosphorothioate modifications against miR-122 (AMO122) were encapsulated in the YSK05-MEND. …
NAID 120005476214

In vitro optimization of 2 '-OMe-4 '-thioribonucleoside-modified anti-microRNA oligonucleotides and its targeting delivery to mouse liver using a liposomal nanoparticle

Takahashi Mayumi,Yamada Naoki,Hatakeyama Hiroto,Murata Manami,Sato Yusuke,Minakawa Noriaki,Harashima Hideyoshi,Matsuda Akira
Nucleic acids research 41(22), 10659-10667, 2013-12
… 2'-O-Methyl (2'-OMe)-4'-thioRNA is a hybrid type of chemically modified oligonucleotide, exhibiting high binding affinity to complementary RNAs and high resistance to nuclease degradation. … Further investigation showed that phosphorothioate modification contributed to long-term miR-122 inhibition by the 2'-OMe-4'-thioribonucleoside-modified AMO. …
NAID 120005394557

Restoration of antibiotic susceptibility in fluoroquinolone-resistant Escherichia coli by targeting acrB with antisense phosphorothioate oligonucleotide encapsulated in novel anion liposome

リンク元	「ホスホロチオエートオリゴヌクレオチド」
関連記事	「phosphorothioated」「oligonucleotide」「phosphorothioate」