Heterologous production of l-lysine ε-oxidase by directed evolution using a fusion reporter method.
Matsui D1, Asano Y.
Bioscience, biotechnology, and biochemistry.Biosci Biotechnol Biochem.2015 Apr 21:1-8. [Epub ahead of print]
For the heterologous production of l-lysine ε-oxidase (LodA), we constructed a new plasmid carrying LodA gene fused in-frame with an antibiotic (phleomycine) resistant gene. The new plasmid was randomly mutated and the mutated plasmids were transformed into Escherichia coli BL21 (DE3) harboring lod
Integrative expression vectors for overexpression of xylitol dehydrogenase (XYL2) in Osmotolerant yeast, Candida glycerinogenes WL2002-5.
Zhang C1, Zong H, Zhuge B, Lu X, Fang H, Zhuge J.
Journal of industrial microbiology & biotechnology.J Ind Microbiol Biotechnol.2015 Jan;42(1):113-24. doi: 10.1007/s10295-014-1530-4. Epub 2014 Nov 4.
Yeasts are excellent hosts for the production of recombinant proteins. Candida glycerinogenes WL2002-5, an osmotolerant yeast with extremely high glycerol productivity, provides an attractive eukaryotic expression platform. The integrative vectors PURGAP-gfp and PURGPD-gfp harbouring phleomycin-resi
BACKGROUND: Transformation of microalgae to obtain recombinant proteins, lipids or metabolites of economic value is of growing interest due to low costs associated with culture growth and scaling up. At present there are only three stable nuclear selection markers for the transformation of Chlamydom
Development of an efficient gene-targeting system in Aspergillus luchuensis by deletion of the non-homologous end joining system(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
Journal of bioscience and bioengineering 112(6), 529-534, 2011-12
InvivoGen provides Phleomycin, a selective antibiotic of the bleomycin family active against most bacteria, filamentous fungi, yeast, plant and animal cells. Phleomycin resistance is conferred by the Sh ble gene.
Phleomycin Selective antibiotic for the Sh ble gene Catalog # ant-ph-1, ant-ph-5, ant-ph-5b For research use only Version # 15A23-MM PRODUCT INFORMATION Content: Phleomycin is supplied as either 1 ml tubes or a 25 ml bottle