Daptomycin antibiotic production processes in fed-batch fermentation by Streptomyces roseosporus NRRL11379 with precursor effect and medium optimization.
Ng IS, Ye C, Zhang Z, Lu Y, Jing K.SourceDepartment of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China, yswu@xmu.edu.cn.
Bioprocess and biosystems engineering.Bioprocess Biosyst Eng.2013 Jul 5. [Epub ahead of print]
Sodium decanoate was first found to be an effective precursor for synthesis of daptomycin from Streptomyces roseosporus NRRL11379 which was increased to 71.55-fold, compared with decanoic acid. The optimal flow rate of precursor was at 600 mg/(L day) after 48 h fermentation. From protein analysis
Intrinsic Acid-Base Properties of a Hexa-2'-deoxynucleoside Pentaphosphate, d(ApGpGpCpCpT): Neighboring Effects and Isomeric Equilibria.
Domínguez-Martín A, Johannsen S, Sigel A, Operschall BP, Song B, Sigel H, Okruszek A, González-Pérez JM, Niclós-Gutiérrez J, Sigel RK.SourceInstitute of Inorganic Chemistry, University of Zürich, Winterthurerstrasse 190, 8057 Zürich (Switzerland), Fax: (+41) 44-635-6802; Department of Inorganic Chemistry, Faculty of Pharmacy, University of Granada, Campus Cartuja, 18071 Granada (Spain).
Chemistry (Weinheim an der Bergstrasse, Germany).Chemistry.2013 Jun 17;19(25):8163-81. doi: 10.1002/chem.201203330. Epub 2013 Apr 17.
The intrinsic acid-base properties of the hexa-2'-deoxynucleoside pentaphosphate, d(ApGpGpCpCpT) [=(A1⋅G2⋅G3⋅C4⋅C5⋅T6)=(HNPP)(5-) ] have been determined by (1) H NMR shift experiments. The pKa values of the individual sites of the adenosine (A), guanosine (G), cytidine (C), and thymidine
Reversible Switching of Fluorophore Property Based on Intrinsic Conformational Transition of Adenylate Kinase during Its Catalytic Cycle.
Fujii A, Hirota S, Matsuo T.AbstractAdenylate kinase shows a conformational transition (OPEN and CLOSED forms) during substrate binding and product release to mediate the phosphoryl transfer between ADP and ATP/AMP. The protein motional characteristics will be useful to construct switching systems of fluorophore properties caused by the catalytic cycle of the enzyme. This paper demonstrates in-situ reversible switching of a fluorophore property driven by the conformational transition of the enzyme. The pyrene-conjugated mutant adenylate kinase is able to switch the monomer/excimer emission property of pyrene on addition of ADP or P1P5-di(adenosine-5')pentaphosphate (Ap5A, a transition state analog). The observation under the dilute condition (~ 0.1 μM) indicates that the emission spectral change was caused by the motion of a protein molecule and not led by protein-protein interactions through π-π stacking of pyrene rings. The switching can be reversibly conducted by using hexokinase-coupling reaction. The fashion of the changes in emission intensities at various ligand concentrations is different between ADP, Mg2+-bound ADP and Mg2+-bound Ap5A. The emission property switching is repeatable by a sequential addition of a substrate in one-pot process. It is proposed that the property of a synthetic molecule on the enzyme surface is switchable in response to the catalytic cycle of adenylate kinase.
Bioconjugate chemistry.Bioconjug Chem.2013 May 29. [Epub ahead of print]
Adenylate kinase shows a conformational transition (OPEN and CLOSED forms) during substrate binding and product release to mediate the phosphoryl transfer between ADP and ATP/AMP. The protein motional characteristics will be useful to construct switching systems of fluorophore properties caused by t
Guanosine tetra- and pentaphosphate synthetase activity in chloroplasts of a higher plant : association with 70S ribosomes and inhibition by tetracycline
pentaphosphate (plural pentaphosphates) A compound having five phosphate groups A polymeric anion composed of five linked phosphate groups; any salt containing this anion See also [] pentakisphosphate Retrieved from "" : ...
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