卵白アルブミン、オボアルブミン、オバルブミン
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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2015/11/16 23:04:04」(JST)
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Ovalbumin (abbreviated OVA[1]) is the main protein found in egg white, making up 60-65% of the total protein.[2] Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily, but unlike most serpins it is not a serine protease inhibitor.[3] The function of ovalbumin is unknown, although it is presumed to be a storage protein.[4]
Contents
- 1 Research
- 2 Structure
- 3 Medicinal characteristics
- 4 See also
- 5 References
- 6 External links
Research
Ovalbumin is an important protein in several different areas of research, including:
- general studies of protein structure and properties (because it is available in large quantities).
- studies of serpin structure and function (the fact that ovalbumin does not inhibit proteases means that by comparing its structure with that of inhibitory serpins, the structural characteristics required for inhibition can be determined).
- proteomics (chicken egg ovalbumin is commonly used as a molecular weight marker for calibrating electrophoresis gels).
- immunology (commonly used to stimulate an allergic reaction in test subjects, e.g. established model allergen for airway hyper-responsiveness, AHR).
(For in vivo and in vitro studies based on ovalbumin it is important that the endotoxin content is less than 1 EU/mg.)[citation needed]
Structure
The ovalbumin protein of chickens is made up of 385 amino acids, and its relative molecular mass is 45 kDa.[5] Ovalbumin also has several modifications, including N-terminal acetylation (G1), phosphorylation (S68, S344), and glycosylation (N292).[5]
It is secreted from the cell. Unlike the signal sequences of most secreted proteins, Ovalbumin's signal sequence is not cleaved. Additionally it is not located directly on the N-terminus, but rather internally, starting at residue 21 and ending at residue 47.[6]
Medicinal characteristics
In cases where poisoning by heavy metals (such as Iron) is suspected, ovalbumin may be administered.[7] Ovalbumin chelates to heavy metals and traps the metal ions within the sulfhydryl bonds of the protein. Chelating prevents the absorption of the metals into the gastrointestinal tract and prevents poisoning.
See also
References
- ^ Sano Kunio; Kanna Haneda; Gen Tamura; Kunio Shirato (1999). "Ovalbumin (OVA) and Mycobacterium tuberculosis Bacilli Cooperatively Polarize Anti-OVA T-helper (Th) Cells toward a Th1-Dominant Phenotype and Ameliorate Murine Tracheal Eosinophilia". Am. J. Respir. Cell Mol. Biol. 20 (6): 1260–1267. doi:10.1165/ajrcmb.20.6.3546. Retrieved 28 December 2011.
- ^ Huntington JA; Stein PE (2001). "Structure and properties of ovalbumin.". Journal of Chromatography B 756 (1-2): 189–198. doi:10.1016/S0378-4347(01)00108-6. PMID 11419711.
- ^ Hu H.Y., Du H.N. (2000). "Alpha to Beta Structural Transformation of Ovalbumin: Heat and pH Effects". Journal of Protein Chemistry 19 (3): 177–183. doi:10.1023/A:1007099502179. PMID 10981809.
- ^ Gettins PGW (2002) Serpin structure, mechanism, and function. Chemical Reviews 102(12): 4751-4804.
- ^ a b Nisbet AD, Saundry RH, Moir AJG, Fothergill LA, Fothergill JE (1981) The complete amino-acid sequence of hen ovalbumin. European Journal of Biochemistry 115(2): 335.
- ^ Robinson A, Meredith C, Austen BM (1986) Isolation and properties of the signal region from ovalbumin. FEBS Letters 203(2): 243-246.
- ^ Dominiczak M, Baynes J, Medical Biochemistry, 2d edition (2004), p59.
External links
- Ovalbumin at the US National Library of Medicine Medical Subject Headings (MeSH)
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Serpins
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| inhibitory |
- Alpha 1-antichymotrypsin
- Alpha 1-antitrypsin
- Alpha 2-antiplasmin
- Antithrombin
- C1-inhibitor
- Heparin cofactor II
- Protein C inhibitor
- Plasminogen activator inhibitor-1
- Plasminogen activator inhibitor-2
- Protein Z-related protease inhibitor
- SERPINA1
- SERPINA2
- SERPINA3
- SERPINA4
- SERPINA5
- SERPINA6
- SERPINA7
- SERPINA8
- SERPINA9
- SERPINA14
- SERPINB1
- SERPINB2
- SERPINB3
- SERPINB4
- SERPINB5
- SERPINB6
- SERPINB7
- SERPINB8
- SERPINB9
- SERPINB13
- SERPINC1
- SERPIND1
- SERPINE1
- SERPINE2
- SERPINE2
- SERPINF1
- SERPING1
- SERPINH1
- SERPINI1
- SERPINI2
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| Cross class inhibitory |
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| noninhibitory |
- Heat shock protein 47
- Maspin
- Ovalbumin
- SERPINF1
- Thyroxine-binding globulin
- Transcortin
- SERPINF1
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|
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see also disorders of globin and globulin proteins
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Proteins: Globular proteins
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| Serum globulins |
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Alpha globulins
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serpins:
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- alpha-1 (Alpha 1-antichymotrypsin, Alpha 1-antitrypsin)
- alpha-2 (Alpha 2-antiplasmin)
- Antithrombin (Heparin cofactor II)
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carrier proteins:
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- alpha-1 (Transcortin)
- alpha-2 (Ceruloplasmin)
- Retinol binding protein
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other:
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- alpha-1 (Orosomucoid)
- alpha-2 (alpha-2-Macroglobulin, Haptoglobin)
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Beta globulins
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carrier proteins:
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- Sex hormone-binding globulin
- Transferrin
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|
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other:
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- Angiostatin
- Hemopexin
- Beta-2 microglobulin
- Factor H
- Plasminogen
- Properdin
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Gamma globulin
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Other
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- Fibronectin (fFN: Fetal fibronectin)
- Macroglobulin/Microglobulin
- Transcobalamin
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| Other globulins |
- Beta-lactoglobulin
- Thyroglobulin
- Alpha-lactalbumin
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| Albumins |
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Egg white
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- Conalbumin
- Ovalbumin
- Avidin
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Serum albumin
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- Human serum albumin
- Bovine serum albumin
- Prealbumin
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Other
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- C-reactive protein
- Lactalbumin (Alpha-lactalbumin)
- Parvalbumin
- Ricin
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- see also disorders of globin and globulin proteins
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Index of proteins
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| Description |
- Proteins
- Membrane
- Globular
- Antibodies
- Fibrous
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UpToDate Contents
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English Journal
- Comparison of glycation in conventionally and microwave-heated ovalbumin by high resolution mass spectrometry.
- Wang H, Tu ZC, Liu GX, Liu CM, Huang XQ, Xiao H.SourceEngineering Research Center for Biomass Conversion, Ministry of Education, Nanchang University, Nanchang, Jiangxi 330047, China.
- Food chemistry.Food Chem.2013 Nov 15;141(2):985-91. doi: 10.1016/j.foodchem.2013.04.045. Epub 2013 Apr 28.
- The glycation extent of ovalbumin under two heating conditions, conventional and microwave heating was monitored by high resolution mass spectrometry, following pepsin digestion. The sequence coverage of the unglycated and glycated ovalbumin was 100% and 95%, respectively. About 35.2% of the lysines
- PMID 23790877
- Human parainfluenza type 3 virus impairs the efficacy of glucocorticoids to limit allergy-induced pulmonary inflammation in guinea-pigs.
- Ford WR, Blair AE, Evans RL, John E, Bugert JJ, Broadley KJ, Kidd EJ.Source*Cardiff School of Pharmacy and Pharmaceutical Sciences, School of Medicine, Cardiff University, Cardiff, U.K.
- Clinical science (London, England : 1979).Clin Sci (Lond).2013 Nov 1;125(10):471-82. doi: 10.1042/CS20130130.
- Viral exacerbations of allergen-induced pulmonary inflammation in pre-clinical models reportedly reduce the efficacy of glucocorticoids to limit pulmonary inflammation and airways hyper-responsiveness to inhaled spasmogens. However, exacerbations of airway obstruction induced by allergen challenge h
- PMID 23678868
- Epigenetic regulation of neural stem cell fate during corticogenesis.
- Muhchyi C, Juliandi B, Matsuda T, Nakashima K.SourceLaboratory of Molecular Neuroscience, Graduate School of Biological Sciences, Nara Institute of Science and Technology, Takayama 8916-5, Ikoma, Nara 630-0192, Japan.
- International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience.Int J Dev Neurosci.2013 Oct;31(6):424-33. doi: 10.1016/j.ijdevneu.2013.02.006. Epub 2013 Mar 4.
- The cerebral cortex comprises over three quarters of the brain, and serves as structural basis for the sophisticated perceptual and cognitive functions. It develops from common multipotent neural stem cells (NSCs) that line the neural tube. Development of the NSCs encompasses sequential phases of pr
- PMID 23466416
Japanese Journal
- The protective effect of α-hederin, the active constituent of Nigella sativa, on tracheal responsiveness and lung inflammation in ovalbumin-sensitized guinea pigs
- Saadat Saeideh,Mohammadi Mostafa,Fallahi Maryam [他]
- The journal of physiological sciences 65(3), 285-292, 2015-05
- NAID 40020444009
- Fucoidan from Macrocystis pyrifera Has Powerful Immune-Modulatory Effects Compared to Three Other Fucoidans
- Zhang Wei,Oda Tatsuya,Yu Qing,Jin Jun-O
- Marine Drugs 13(3), 1084-1104, 2015-02-19
- … pyrifera fucoidan induced the strongest activation of spleen DCs and T cells and ovalbumin (OVA) specific immune responses compared to other fucoidans. …
- NAID 120005603866
- 脂肪酸塩誘導卵白アルブミンゲルの形成に及ぼす魚肉水溶性タンパク質添加の効果
- 太田 尚子
- 日本海水学会誌 = Bulletin of the Society of Sea Water Science, Japan 69(1), 49-52, 2015-02
- NAID 40020344479
Related Links
- Ovalbumin (abbreviated OVA) is the main protein found in egg white, making up 60-65% of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily, but unlike most serpins it is not a serine ...
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