nutrient agar

• 栄養寒天

WordNet

1. a colloidal extract of algae; used especially in culture media and as a gelling agent in foods (同)agar-agar
2. any culture medium that uses agar as the gelling agent (同)nutrient_agar
3. any substance (such as a chemical element or inorganic compound) that can be taken in by a green plant and used in organic synthesis

PrepTutorEJDIC

1. 寒天;(細菌の培養などに用いる)寒天培養基
2. 栄養に富む / 〈C〉栄養物,栄養剤

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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2015/11/27 20:09:12」(JST)

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Nutrient agar is a general purpose medium supporting growth of a wide range of non-fastidious organisms. It typically contains (mass/volume):

• 0.5% Peptone - this provides organic nitrogen
• 0.3% beef extract/yeast extract - the water-soluble content of these contribute vitamins, carbohydrates, nitrogen, and salts
• 1.5% agar - this gives the mixture solidity
• 0.5% Sodium Chloride - this gives the mixture proportions similar to those found in the cytoplasm of most organisms
• distilled water - water serves as a transport medium for the agar's various substances
• pH adjusted to neutral (6.8) at 25 °C.

These ingredients are combined and boiled for approximately one minute to ensure they are mixed and to sterilize them. Then they are cooled to around 50 °C (122 °F) and poured into Petri dishes which are covered immediately. Once the dishes hold solidified agar, they are stored upside down and are often refrigerated until used. Inoculation takes place on warm dishes rather than cool ones: if refrigerated for storage, the dishes must be rewarmed to room temperature prior to inoculation.

See also

• Plate count agar

References

Further reading

• Lapage S., Shelton J. and Mitchell T., 1970, Methods in Microbiology', Norris J. and Ribbons D., (Eds.), Vol. 3A, Academic Press, London.
• MacFaddin J. F., 2000, Biochemical Tests for Identification of Medical Bacteria, 3rd Ed., Lippincott, Williams and Wilkins, Baltimore.
• Downes F. P. and Ito K., (Ed.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., American Public Health Association, Washington, D.C.
• American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed., Washington D.C.

UpToDate Contents

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English Journal

• Properties and characterization of agar/CuNP bionanocomposite films prepared with different copper salts and reducing agents.

• Shankar S1, Teng X1, Rhim JW2.
• Carbohydrate polymers.Carbohydr Polym.2014 Dec 19;114:484-92. doi: 10.1016/j.carbpol.2014.08.036. Epub 2014 Aug 27.
• Various types of agar-based bio-nanocomposite (BNC) films were prepared by blending agar and six different copper nanoparticles (CuNPs) with different shapes and sizes obtained from three different sources of copper salts and two different reducing agents. The BNC films were characterized by UV-visi
• PMID 25263917

• Antimicrobial Susceptibility Profiles and Molecular Typing of Campylobacter jejuni and Campylobacter coli Isolates from Ducks in South Korea.

• Wei B1, Cha SY1, Kang M1, Roh JH1, Seo HS1, Yoon RH1, Jang HK2.
• Applied and environmental microbiology.Appl Environ Microbiol.2014 Dec 15;80(24):7604-10. doi: 10.1128/AEM.02469-14. Epub 2014 Sep 26.
• Campylobacter is a food-borne zoonotic pathogen that causes human gastroenteritis worldwide. Campylobacter bacteria are commensal in the intestines of many food production animals, including ducks and chickens. The objective of the study was to determine the prevalence of Campylobacter species in do
• PMID 25261524

• Characterization of the emerging clinically-relevant multidrug-resistant Salmonella enterica serotype 4,[5],12:i:- (monophasic variant of S. Typhimurium) clones.

• Mourão J1, Machado J, Novais C, Antunes P, Peixe L.
• European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology.Eur J Clin Microbiol Infect Dis.2014 Dec;33(12):2249-57. doi: 10.1007/s10096-014-2180-1. Epub 2014 Jul 15.
• To better understand the recent success/emergence of Salmonella enterica serotype 4,[5],12:i:- we characterized the population diversity, fljAB deletion patterns, antibiotic resistance features and associated genetic elements of a comprehensive collection obtained in the last decade from Portugal (2
• PMID 25022446

Japanese Journal

• Nutrient agar with sodium chloride supplementation for presumptive detection of Moraxella catarrhalis in clinical specimens

• NISHIYAMA Hiroyuki,SAITO Ryoichi,CHIDA Toshio,SANO Kazumitsu,TSUCHIYA Tatsuyuki,OKAMURA Noboru
• Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy 18(2), 219-227, 2012-04-01
• NAID 10030545656

• 鮮魚の35℃培養公定法による生菌数と20℃細菌培養法による生菌数の比較

• 福田 翼,古下 学,芝 恒男
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• 海産の鮮魚を汚染する細菌には、生息環境由来の好塩性低温細菌や沿岸域に流入する陸棲の非好塩性細菌、製造・流通過程で汚染する手指や器具由来の細菌群がある。食品衛生法で定める検査方法（公定法）ではNaClを含まない標準寒天培地を使用し、好気的条件下で35±1。0℃で48±3時間培養を行うこととなっているが、35℃培養法よりも20℃培養法の方の細菌計数値が高く、標準寒天培地よりもNaClを含有している普通 …
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• シイタケ腐敗病菌Ewingella americanaの分離用選択培地

• 有馬 忍,篠原 弘亮,根岸 寛光
• 日本きのこ学会誌 : mushroom science and biotechnology 19(4), 181-186, 2012-01-31
• シイタケ腐敗病細菌Ewingella americanaの選択培地を作製した.本培地(A-D3培地と命名)は蒸留水1,000mLにD(+)アラビトール10g,カザミノ酸ビタミンフリー5g,塩化リチウム7g,塩化ナトリウム5g,硫酸マグネシウム七水和物0.3g,ドデシル硫酸ナトリウム50mg,0.2%ブロモチモールブルー15mL,寒天15gの組成で,pH7.2に調整した.シイタケ病原細菌Ew. am …
• NAID 110009470738

Related Links

• Nutrient Agar 普通寒天

358. Merck Microbiology Manual 12th Edition. Nutrient Agar 普通寒天. 発育が あまり困難でない微生物の培養のための広範囲な培地。 本培地は、APHA の乳製品 の検査法（1985) に準拠。 組成 (g/litre). カゼインペプトン 5.0; 酵母エキス 3.0; 寒天 12.0.

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• 寒天、カンテン