nucleolin

Nucleolin
	PDB rendering based on 2fc8.
Available structures
PDB	Ortholog search: PDBe, RCSB
List of PDB id codes
	2FC8, 2FC9, 2KRR
Identifiers
Symbols	NCL ; C23
External IDs	OMIM: 164035 HomoloGene: 136488 GeneCards: NCL Gene
Gene ontology
Molecular function	• nucleotide binding; • single-stranded DNA binding; • RNA binding; • calcium ion binding; • protein binding; • protein C-terminus binding; • selenocysteine insertion sequence binding; • rRNA primary transcript binding; • telomeric DNA binding; • histone binding; • identical protein binding; • laminin binding; • poly(A) RNA binding; • ErbB-4 class receptor binding;
Cellular component	• fibrillar center; • dense fibrillar component; • nucleus; • nucleoplasm; • nucleolus; • cell cortex; • cell surface; • membrane; • ribonucleoprotein complex; • cytoplasmic ribonucleoprotein granule; • extracellular exosome;
Biological process	• angiogenesis; • endocytosis; • spermatogenesis; • positive regulation of tumor necrosis factor production; • negative regulation of apoptotic process; • positive regulation of transcription from RNA polymerase II promoter; • cellular response to lipopolysaccharide; • liver regeneration; • positive regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter; • regulation of rRNA processing; • positive regulation of interleukin-6 secretion;
	Sources: Amigo / QuickGO
RNA expression pattern
	More reference expression data
Orthologs
	v; t; e;

n.

(核小体構成タンパク質)ヌクレオリン

Wikipedia preview

出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2016/05/01 21:42:43」(JST)

wiki en

[Wiki en表示]

Nucleolin is a protein that in humans is encoded by the NCL gene.^[1]^[2]

Gene

The human NCL gene is located on chromosome 2 and consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.^[3]

Function

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes.^[3] The protein is located mainly in dense fibrillar regions of the nucleolus.

Nucleolin is also able to act as a transcriptional coactivator with Chicken Ovalbumin Upstream Promoter Transcription Factor II (COUP-TFII).^[4]

Clinical significance

Midkine and pleiotrophin bind to cell-surface nucleolin as a low affinity receptor. This binding can inhibit HIV infection.^[5]^[6]

Nucleolin at the cell surface is the receptor for the Respiratory Syncytial Virus (RSV) fusion protein.^[7] Interference with the nucleolin - RSV fusion protein interaction has been shown to be therapeutic against RSV infection in cell cultures and animal models.^[8]^[9]^[10]

Interactions

Nucleolin has been shown to interact with:

CSNK2A2,^[11]
Centaurin, alpha 1,^[12]
HuR,^[13]
NPM1,^[14]
P53,^[15]
PPP1CB,^[16]
S100A11,^[17]
Sjogren syndrome antigen B,^[18]
TOP1,^[19]^[20] and
Telomerase reverse transcriptase.^[21]

References

^ Srivastava M, McBride OW, Fleming PJ, Pollard HB, Burns AL (Sep 1990). "Genomic organization and chromosomal localization of the human nucleolin gene". The Journal of Biological Chemistry 265 (25): 14922–31. PMID 2394707.
^ Erard MS, Belenguer P, Caizergues-Ferrer M, Pantaloni A, Amalric F (Aug 1988). "A major nucleolar protein, nucleolin, induces chromatin decondensation by binding to histone H1". European Journal of Biochemistry / FEBS 175 (3): 525–30. doi:10.1111/j.1432-1033.1988.tb14224.x. PMID 3409881.
^ ^a ^b "Entrez Gene: NCL nucleolin".
^ Litchfield LM, Riggs KA, Hockenberry AM, Oliver LD, Barnhart KG, Cai J, Pierce WM, Ivanova MM, Bates PJ, Appana SN, Datta S, Kulesza P, McBryan J, Young LS, Klinge CM (May 2012). "Identification and characterization of nucleolin as a COUP-TFII coactivator of retinoic acid receptor β transcription in breast cancer cells". PLOS ONE 7 (5): e38278. doi:10.1371/journal.pone.0038278. PMC 3365040. PMID 22693611.
^ Said EA, Krust B, Nisole S, Svab J, Briand JP, Hovanessian AG (Oct 2002). "The anti-HIV cytokine midkine binds the cell surface-expressed nucleolin as a low affinity receptor". The Journal of Biological Chemistry 277 (40): 37492–502. doi:10.1074/jbc.M201194200. PMID 12147681.
^ Said EA, Courty J, Svab J, Delbé J, Krust B, Hovanessian AG (Sep 2005). "Pleiotrophin inhibits HIV infection by binding the cell surface-expressed nucleolin". The FEBS Journal 272 (18): 4646–59. doi:10.1111/j.1742-4658.2005.04870.x. PMID 16156786.
^ Tayyari F, Marchant D, Moraes TJ, Duan W, Mastrangelo P, Hegele RG (Sep 2011). "Identification of nucleolin as a cellular receptor for human respiratory syncytial virus". Nature Medicine 17 (9): 1132–5. doi:10.1038/nm.2444. PMID 21841784.
^ Mastrangelo P, Hegele RG (Nov 2012). "The RSV fusion receptor: not what everyone expected it to be". Microbes and Infection / Institut Pasteur 14 (13): 1205–10. doi:10.1016/j.micinf.2012.07.015. PMID 22884716.
^ Mastrangelo P, Hegele RG (Mar 2013). "RSV fusion: time for a new model". Viruses 5 (3): 873–85. doi:10.3390/v5030873. PMID 23518574.
^ Shakeri A, Mastrangelo P, Griffin JK, Moraes TJ, Hegele RG (Nov 2014). "Respiratory syncytial virus receptor expression in the mouse and viral tropism". Histology and Histopathology 30: 401–411. PMID 25374027.
^ Li D, Dobrowolska G, Krebs EG (Jun 1996). "The physical association of casein kinase 2 with nucleolin". The Journal of Biological Chemistry 271 (26): 15662–8. doi:10.1074/jbc.271.26.15662. PMID 8663258.
^ Dubois T, Zemlickova E, Howell S, Aitken A (Feb 2003). "Centaurin-alpha 1 associates in vitro and in vivo with nucleolin". Biochemical and Biophysical Research Communications 301 (2): 502–8. doi:10.1016/s0006-291x(02)03010-3. PMID 12565890.
^ Tominaga K, Srikantan S, Lee EK, Subaran SS, Martindale JL, Abdelmohsen K, Gorospe M (Oct 2011). "Competitive regulation of nucleolin expression by HuR and miR-494". Molecular and Cellular Biology 31 (20): 4219–31. doi:10.1128/MCB.05955-11. PMC 3187287. PMID 21859890.
^ Li YP, Busch RK, Valdez BC, Busch H (Apr 1996). "C23 interacts with B23, a putative nucleolar-localization-signal-binding protein". European Journal of Biochemistry / FEBS 237 (1): 153–8. doi:10.1111/j.1432-1033.1996.0153n.x. PMID 8620867.
^ Daniely Y, Dimitrova DD, Borowiec JA (Aug 2002). "Stress-dependent nucleolin mobilization mediated by p53-nucleolin complex formation". Molecular and Cellular Biology 22 (16): 6014–22. doi:10.1128/mcb.22.16.6014-6022.2002. PMC 133981. PMID 12138209.
^ Morimoto H, Okamura H, Haneji T (Sep 2002). "Interaction of protein phosphatase 1 delta with nucleolin in human osteoblastic cells". The Journal of Histochemistry and Cytochemistry 50 (9): 1187–93. doi:10.1177/002215540205000905. PMID 12185196.
^ Sakaguchi M, Miyazaki M, Takaishi M, Sakaguchi Y, Makino E, Kataoka N, Yamada H, Namba M, Huh NH (Nov 2003). "S100C/A11 is a key mediator of Ca(2+)-induced growth inhibition of human epidermal keratinocytes". The Journal of Cell Biology 163 (4): 825–35. doi:10.1083/jcb.200304017. PMC 2173690. PMID 14623863.
^ Fouraux MA, Bouvet P, Verkaart S, van Venrooij WJ, Pruijn GJ (Jul 2002). "Nucleolin associates with a subset of the human Ro ribonucleoprotein complexes". Journal of Molecular Biology 320 (3): 475–88. doi:10.1016/s0022-2836(02)00518-1. PMID 12096904.
^ Haluska P, Saleem A, Edwards TK, Rubin EH (Apr 1998). "Interaction between the N-terminus of human topoisomerase I and SV40 large T antigen". Nucleic Acids Research 26 (7): 1841–7. doi:10.1093/nar/26.7.1841. PMC 147454. PMID 9512561.
^ Bharti AK, Olson MO, Kufe DW, Rubin EH (Jan 1996). "Identification of a nucleolin binding site in human topoisomerase I". The Journal of Biological Chemistry 271 (4): 1993–7. doi:10.1074/jbc.271.4.1993. PMID 8567649.
^ Khurts S, Masutomi K, Delgermaa L, Arai K, Oishi N, Mizuno H, Hayashi N, Hahn WC, Murakami S (Dec 2004). "Nucleolin interacts with telomerase". The Journal of Biological Chemistry 279 (49): 51508–15. doi:10.1074/jbc.M407643200. PMID 15371412.

UpToDate Contents

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1. 腸管出血性大腸菌（EHEC）の微生物学、病因、疫学、および予防 microbiology pathogenesis epidemiology and prevention of enterohemorrhagic escherichia coli ehec
2. 筋萎縮性側索硬化症の疫学および病因 epidemiology and pathogenesis of amyotrophic lateral sclerosis

English Journal

An AS1411 aptamer-conjugated liposomal system containing a bubble-generating agent for tumor-specific chemotherapy that overcomes multidrug resistance.

Liao ZX1, Chuang EY2, Lin CC2, Ho YC3, Lin KJ4, Cheng PY2, Chen KJ2, Wei HJ5, Sung HW6.
Journal of controlled release : official journal of the Controlled Release Society.J Control Release.2015 Jun 28;208:42-51. doi: 10.1016/j.jconrel.2015.01.032. Epub 2015 Jan 28.
Recent research in chemotherapy has prioritized overcoming the multidrug resistance (MDR) of cancer cells. In this work, liposomes that contain doxorubicin (DOX) and ammonium bicarbonate (ABC, a bubble-generating agent) are prepared and functionalized with an antinucleolin aptamer (AS1411 liposomes)
PMID 25637705

AS1411-conjugated gold nanospheres and their potential for breast cancer therapy.

Malik MT1,2, O'Toole MG3, Casson LK1,2, Thomas SD1,2, Bardi GT1,2, Reyes-Reyes EM1,2, Ng CK4, Kang KA5, Bates PJ1,2.
Oncotarget.Oncotarget.2015 Jun 3. [Epub ahead of print]
AS1411 is a quadruplex-forming DNA oligonucleotide that functions as an aptamer to target nucleolin, a protein present on the surface of cancer cells. Clinical trials of AS1411 have indicated it is well tolerated with evidence of therapeutic activity, but improved pharmacology and potency may be req
PMID 26045302

The roles of nucleolin subcellular localization in cancer.

Berger CM1, Gaume X2, Bouvet P3.
Biochimie.Biochimie.2015 Jun;113:78-85. doi: 10.1016/j.biochi.2015.03.023. Epub 2015 Apr 9.
Nucleolin (NCL) is one of the most abundant non ribosomal protein of the nucleolus where it plays a central role in polymerase I transcription. NCL is also found outside of the nucleolus, in the nucleoplasm, cytoplasm as well as on the cell membrane. It acts in all cell compartments to control cellu
PMID 25866190

Japanese Journal

Nucleolin Acts as a Scavenger Receptor for Acetylated Low-Density Lipoprotein on Macrophages

Miki Yuichi,Tachibana Yoshihiro,Ohminato Yukari [他],Fujiwara Yasuyuki
Biological and Pharmaceutical Bulletin 38(9), 1420-1424, 2015
… We recently showed that nucleolin expressed on macrophages acts as a scavenger receptor for various endogenous discarded products. … Here, we investigated whether or not nucleolin is involved in the uptake of acetylated LDL (AcLDL). … In contrast to normal LDL, AcLDL directly bound to immobilized nucleolin. …
NAID 130005096985

Nucleolin Is a Receptor for Maleylated-Bovine Serum Albumin on Macrophages

Miki Yuichi,Koyama Keisuke,Kurusu Haruna [他],Hirano Kazuya,Beppu Masatoshi,Fujiwara Yasuyuki
Biological and Pharmaceutical Bulletin 38(1), 116-121, 2015
… The shuttling-protein nucleolin has recently been shown to possess scavenger receptor-like activity. … We therefore investigated whether or not nucleolin is a receptor for maleylated-bovine serum albumin (maleylated-BSA), which is a common ligand for scavenger receptors. … Surface plasmon-resonance analysis revealed that nucleolin strongly associated with maleylated-BSA but not control-BSA or maleic anhydride. …
NAID 130004872219

Macrophage Recognition of Toxic Advanced Glycosylation End Products through the Macrophage Surface-Receptor Nucleolin

Miki Yuichi,Dambara Hikaru,Tachibana Yoshihiro [他]
Biological & pharmaceutical bulletin 37(4), 588-596, 2014-04
NAID 40020030162

Related Pictures

[pmid2394707-1] Srivastava M, McBride OW, Fleming PJ, Pollard HB, Burns AL (Sep 1990). "Genomic organization and chromosomal localization of the human nucleolin gene". The Journal of Biological Chemistry 265 (25): 14922–31. PMID 2394707.

[pmid3409881-2] Erard MS, Belenguer P, Caizergues-Ferrer M, Pantaloni A, Amalric F (Aug 1988). "A major nucleolar protein, nucleolin, induces chromatin decondensation by binding to histone H1". European Journal of Biochemistry / FEBS 175 (3): 525–30. doi:10.1111/j.1432-1033.1988.tb14224.x. PMID 3409881.

[entrez-3] "Entrez Gene: NCL nucleolin".

[pmid22693611-4] Litchfield LM, Riggs KA, Hockenberry AM, Oliver LD, Barnhart KG, Cai J, Pierce WM, Ivanova MM, Bates PJ, Appana SN, Datta S, Kulesza P, McBryan J, Young LS, Klinge CM (May 2012). "Identification and characterization of nucleolin as a COUP-TFII coactivator of retinoic acid receptor β transcription in breast cancer cells". PLOS ONE 7 (5): e38278. doi:10.1371/journal.pone.0038278. PMC 3365040. PMID 22693611.

[pmid12147681-5] Said EA, Krust B, Nisole S, Svab J, Briand JP, Hovanessian AG (Oct 2002). "The anti-HIV cytokine midkine binds the cell surface-expressed nucleolin as a low affinity receptor". The Journal of Biological Chemistry 277 (40): 37492–502. doi:10.1074/jbc.M201194200. PMID 12147681.

[pmid16156786-6] Said EA, Courty J, Svab J, Delbé J, Krust B, Hovanessian AG (Sep 2005). "Pleiotrophin inhibits HIV infection by binding the cell surface-expressed nucleolin". The FEBS Journal 272 (18): 4646–59. doi:10.1111/j.1742-4658.2005.04870.x. PMID 16156786.

[pmid21841784-7] Tayyari F, Marchant D, Moraes TJ, Duan W, Mastrangelo P, Hegele RG (Sep 2011). "Identification of nucleolin as a cellular receptor for human respiratory syncytial virus". Nature Medicine 17 (9): 1132–5. doi:10.1038/nm.2444. PMID 21841784.

[pmid22884716-8] Mastrangelo P, Hegele RG (Nov 2012). "The RSV fusion receptor: not what everyone expected it to be". Microbes and Infection / Institut Pasteur 14 (13): 1205–10. doi:10.1016/j.micinf.2012.07.015. PMID 22884716.

[pmid23518574-9] Mastrangelo P, Hegele RG (Mar 2013). "RSV fusion: time for a new model". Viruses 5 (3): 873–85. doi:10.3390/v5030873. PMID 23518574.

[pmid25374027-10] Shakeri A, Mastrangelo P, Griffin JK, Moraes TJ, Hegele RG (Nov 2014). "Respiratory syncytial virus receptor expression in the mouse and viral tropism". Histology and Histopathology 30: 401–411. PMID 25374027.

[pmid8663258-11] Li D, Dobrowolska G, Krebs EG (Jun 1996). "The physical association of casein kinase 2 with nucleolin". The Journal of Biological Chemistry 271 (26): 15662–8. doi:10.1074/jbc.271.26.15662. PMID 8663258.

[pmid12565890-12] Dubois T, Zemlickova E, Howell S, Aitken A (Feb 2003). "Centaurin-alpha 1 associates in vitro and in vivo with nucleolin". Biochemical and Biophysical Research Communications 301 (2): 502–8. doi:10.1016/s0006-291x(02)03010-3. PMID 12565890.

[pmid21859890-13] Tominaga K, Srikantan S, Lee EK, Subaran SS, Martindale JL, Abdelmohsen K, Gorospe M (Oct 2011). "Competitive regulation of nucleolin expression by HuR and miR-494". Molecular and Cellular Biology 31 (20): 4219–31. doi:10.1128/MCB.05955-11. PMC 3187287. PMID 21859890.

[pmid8620867-14] Li YP, Busch RK, Valdez BC, Busch H (Apr 1996). "C23 interacts with B23, a putative nucleolar-localization-signal-binding protein". European Journal of Biochemistry / FEBS 237 (1): 153–8. doi:10.1111/j.1432-1033.1996.0153n.x. PMID 8620867.

[pmid12138209-15] Daniely Y, Dimitrova DD, Borowiec JA (Aug 2002). "Stress-dependent nucleolin mobilization mediated by p53-nucleolin complex formation". Molecular and Cellular Biology 22 (16): 6014–22. doi:10.1128/mcb.22.16.6014-6022.2002. PMC 133981. PMID 12138209.

[pmid12185196-16] Morimoto H, Okamura H, Haneji T (Sep 2002). "Interaction of protein phosphatase 1 delta with nucleolin in human osteoblastic cells". The Journal of Histochemistry and Cytochemistry 50 (9): 1187–93. doi:10.1177/002215540205000905. PMID 12185196.

[pmid14623863-17] Sakaguchi M, Miyazaki M, Takaishi M, Sakaguchi Y, Makino E, Kataoka N, Yamada H, Namba M, Huh NH (Nov 2003). "S100C/A11 is a key mediator of Ca(2+)-induced growth inhibition of human epidermal keratinocytes". The Journal of Cell Biology 163 (4): 825–35. doi:10.1083/jcb.200304017. PMC 2173690. PMID 14623863.

[pmid12096904-18] Fouraux MA, Bouvet P, Verkaart S, van Venrooij WJ, Pruijn GJ (Jul 2002). "Nucleolin associates with a subset of the human Ro ribonucleoprotein complexes". Journal of Molecular Biology 320 (3): 475–88. doi:10.1016/s0022-2836(02)00518-1. PMID 12096904.

[pmid9512561-19] Haluska P, Saleem A, Edwards TK, Rubin EH (Apr 1998). "Interaction between the N-terminus of human topoisomerase I and SV40 large T antigen". Nucleic Acids Research 26 (7): 1841–7. doi:10.1093/nar/26.7.1841. PMC 147454. PMID 9512561.

[pmid8567649-20] Bharti AK, Olson MO, Kufe DW, Rubin EH (Jan 1996). "Identification of a nucleolin binding site in human topoisomerase I". The Journal of Biological Chemistry 271 (4): 1993–7. doi:10.1074/jbc.271.4.1993. PMID 8567649.

[pmid15371412-21] Khurts S, Masutomi K, Delgermaa L, Arai K, Oishi N, Mizuno H, Hayashi N, Hahn WC, Murakami S (Dec 2004). "Nucleolin interacts with telomerase". The Journal of Biological Chemistry 279 (49): 51508–15. doi:10.1074/jbc.M407643200. PMID 15371412.

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