English Journal

In vitro fertilization in pigs: New molecules and protocols to consider in the forthcoming years.

Romar R1, Funahashi H2, Coy P3.
Theriogenology.Theriogenology.2016 Jan 1;85(1):125-134. doi: 10.1016/j.theriogenology.2015.07.017. Epub 2015 Jul 23.
Assisted reproduction technology (ART) protocols are used in livestock for the improvement and preservation of their genetics and to enhance reproductive efficiency. In the case of pigs, the potential use of embryos for biomedicine is being followed with great interest by the scientific community. O
PMID 26271164

A phosphodiesterase type-5 inhibitor, sildenafil, induces sperm capacitation and penetration into porcine oocytes in a chemically defined medium.

Ioki S1, Wu QS1, Takayama O2, Motohashi HH2, Wakai T1, Funahashi H3.
Theriogenology.Theriogenology.2015 Sep 12. pii: S0093-691X(15)00484-7. doi: 10.1016/j.theriogenology.2015.09.013. [Epub ahead of print]
The present study was undertaken to determine the effect of a phosphodiesterase (PDE) type-5 (cyclic guanosine monophosphate-specific) inhibitor, sildenafil, on capacitation and penetration of boar spermatozoa in a basic chemically defined medium (adenosine- and theophylline-free PGM-tac4). When eja
PMID 26443234

Timing of oviductal fluid collection, steroid concentrations, and sperm preservation method affect porcine in vitro fertilization efficiency.

Ballester L1, Romero-Aguirregomezcorta J1, Soriano-Úbeda C1, Matás C1, Romar R1, Coy P2.
Fertility and sterility.Fertil Steril.2014 Dec;102(6):1762-8.e1. doi: 10.1016/j.fertnstert.2014.08.009. Epub 2014 Sep 17.
OBJECTIVE: To determine optimal conditions for the inclusion of oviductal fluid (OF) in IVF protocols.DESIGN: Experimental prospective study.SETTING: Mammalian reproduction research laboratory.ANIMAL(S): Oviducts and ovaries from porcine females were collected at a slaughterhouse. A total of 30 ovid
PMID 25241366