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English Journal

Combination of oviduct fluid and heparin to improve monospermic zygotes production during porcine in vitro fertilization.

Batista RI1, Moro LN2, Corbin E2, Alminana C2, Souza-Fabjan JM3, de Figueirêdo Freitas VJ3, Mermillod P2.
Theriogenology.Theriogenology.2016 Jul 15;86(2):495-502. doi: 10.1016/j.theriogenology.2016.01.031. Epub 2016 Feb 9.
In vivo, the oviduct provides appropriate microenvironment conditions for monospermic fertilization and early embryo development. In addition, glycosaminoglycans such as heparin are present in the oviduct and have been shown to modulate the activity of oviduct-secreted proteins on the regulation of
PMID 26964763

Separation of motile sperm for in vitro fertilization from frozen-thawed bull semen using progesterone induction on a microchip.

Li J1, Ning B1, Cao X2, Luo Y1, Guo L1, Wei G1, Liu S1, Zhang Y1, Zhang A1, Wu R1, Li Y3.
Animal reproduction science.Anim Reprod Sci.2016 Jul 5. pii: S0378-4320(16)30276-7. doi: 10.1016/j.anireprosci.2016.07.002. [Epub ahead of print]
This study presents a novel method for the separation of motile sperm from non-progressive motile and immotile sperm and in vitro Fertilization (IVF). This separation of bull sperm was accomplished by inducing chemotaxis along a progesterone release agent in a 7.5-mm microchannel microchip composed
PMID 27452154

Application of a microfluidic sperm sorter to in vitro production of dairy cattle sex-sorted embryos.

Li J1, Zhu S1, He X1, Sun R1, He Q1, Gan Y1, Liu S1, Funahashi H2, Li Y3.
Theriogenology.Theriogenology.2016 Apr 15;85(7):1211-8. doi: 10.1016/j.theriogenology.2015.12.001. Epub 2015 Dec 13.
Viable sperm from sex-sorted semen without centrifugal treatment was separated by a microfluidic sperm sorter (MFSS) for IVF to improve in vitro embryo production of dairy cattle. The MFSS was originally developed to isolate motile human sperm by two laminar flows in the micro-channel (there are fo
PMID 26768540

Application of a microfluidic sperm sorter to the in-vitro fertilization of porcine oocytes reduced the incidence of polyspermic penetration

Sano Hikaru,Matsuura Koji,Naruse Keiji,Funahashi Hiroaki
Theriogenology 74(5), 863-870, 2010-09-15
… In Experiment 2, when porcine IVM oocytes were placed at three locations in the MFSS exit chamber (where only motile spermatozoa accumulated) and subsequently cultured in caffeine-free mM199 for 8 h, sperm penetration rate was not significantly different among places (86.1 +/- 10.5 to 100%), but the monospermic penetration rate was lower (P < … In Experiment 3, the normal fertilization index (ratio of monospermic oocytes to number of oocytes examined) 8 h after insemination was higher (P < …
NAID 120004897828

河村和弘
日本産科婦人科學會雜誌 61(10), 1935-1944, 2009-10-01
… In addition to nuclear maturation exemplified by GVBD and extrusion of the first polar body to complete the first meiotic division, oocytes also undergo cytoplasmic maturation characterized by cytoplasmic changes essential for monospermic fertilization, processing of the sperm, and preparation for development to preimplantation embryos. …
NAID 110007387119

The Distribution of Actin Filaments in the Egg of the Rose Bitterling during the Sperm Entry Process in Monospermic Fertilization

太田忠之,山田雅人,廣田智史
愛知教育大学研究報告自然科学 58, 35-41, 2009-03-00
… The distribution of actin filaments and morphological changes in the portion of egg cortex（PAS）at and around the sperm entry site during sperm entry in eggs of a teleost undergoing monospermic fertilization were investigated by using fluorescence and scanning electron microscopy. …
NAID 120001249986