関: genetrap

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(genetics) a segment of DNA that is involved in producing a polypeptide chain; it can include regions preceding and following the coding DNA as well as introns between the exons; it is considered a unit of heredity; "genes were formerly called factors" (同)cistron, factor
informal term for information; "give me the gen on your new line of computers"

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(地位を示すために衣服などに付ける)飾り,装師 / 装飾的な馬具,馬飾り

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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2012/05/12 00:56:28」(JST)

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Gene trapping is a high-throughput approach that is used to introduce insertional mutations across the mammalian genome. It is performed with gene trap vectors whose principal element is a gene trapping cassette consisting of a promoterless reporter gene and/or selectable genetic marker flanked by an upstream 3’ splice site (splice acceptor; SA) and a downstream transcriptional termination sequence (polyadenylation sequence; polyA). When inserted into an intron of an expressed gene, the gene trap cassette is transcribed from the endogenous promoter of that gene in the form of a fusion transcript in which the exon(s) upstream of the insertion site is spliced in frame to the reporter/selectable marker gene. Since transcription is terminated prematurely at the inserted polyadenylation site, the processed fusion transcript encodes a truncated and non-functional version of the cellular protein and the reporter/selectable marker. Thus, gene traps simultaneously inactivate and report the expression of the trapped gene at the insertion site, and provide a DNA tag (gene trap sequence tag, GTST) for the rapid identification of the disrupted gene. An international public consortium International Gene Trap Consortium is centralizing the data and cell lines can be requested from them.

References

Gossler, A., A. L. Joyner, et al. (1989). "Mouse embryonic stem cells and reporter constructs to detect developmentally regulated genes." Science 244(4903): 463-5.
von Melchner, H. and H. E. Ruley (1989). "Identification of cellular promoters by using a retrovirus promoter trap." J Virol 63(8): 3227-33.
Friedrich, G. and P. Soriano (1991) "Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice." Genes Dev 5:1413-1523.
Zambrowicz, B. P., G. A. Friedrich, et al. (1998). "Disruption and sequence identification of 2,000 genes in mouse embryonic stem cells." Nature 392(6676): 608-11.
Wiles, M. V., F. Vauti, et al. (2000). "Establishment of a gene-trap sequence tag library to generate mutant mice from embryonic stem cells." Nat Genet 24(1): 13-4.
Gene Trap Mutagenesis past, present & beyond; Stanford WL, Cohn JB, Cordes SP (2001)Nature Rev. Genet., 2, 756–768
Skarnes, W. C., H. von Melchner, et al. (2004). "A public gene trap resource for mouse functional genomics." Nat Genet 36(6): 543-4.
Hansen, J., T. Floss, et al. (2003). "A large-scale, gene-driven mutagenesis approach for the functional analysis of the mouse genome." Proc Natl Acad Sci U S A 100(17): 9918-22.
Zambrowicz, B. P., A. Abuin, et al. (2003). "Wnk1 kinase deficiency lowers blood pressure in mice: A gene-trap screen to identify potential targets for therapeutic intervention." Proc Natl Acad Sci U S A 100(24): 14109-14.
Schnutgen, F., S. De-Zolt, et al. (2005). "Genomewide production of multipurpose alleles for the functional analysis of the mouse genome." Proc Natl Acad Sci U S A 102(20): 7221-
De-Zolt, S., F. Schnutgen, et al. (2006). "High-throughput trapping of secretory pathway genes in mouse embryonic stem cells." Nucleic Acids Res 34(3): e25.
Patricia S. Springer (2000). "Gene Traps: Tools for Plant Development and Genomics." The Plant Cell, Vol. 12:1007-1020.

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English Journal

Vinculin functions as regulator of chondrogenesis.

Koshimizu T, Kawai M, Kondou H, Tachikawa K, Sakai N, Ozono K, Michigami T.SourceFrom the Department of Bone and Mineral Research, Osaka Medical Center and Research Institute for Maternal and Child Health, 840 Murodo-cho, Izumi, Osaka 594-1101, Japan and.
The Journal of biological chemistry.J Biol Chem.2012 May 4;287(19):15760-75. Epub 2012 Mar 13.
To identify the genes involved in chondrocytic differentiation, we applied gene trap mutagenesis to a murine mesenchymal chondrogenic cell line ATDC5 and isolated a clone in which the gene encoding vinculin was trapped. The trapped allele was assumed to express a fusion protein containing a truncate
PMID 22416133

A field test for host fruit odour discrimination and avoidance behaviour for Rhagoletis pomonella flies in the western United States.

Sim SB, Mattsson M, Feder JL, Cha DH, Yee WL, Goughnour RB, Linn CE Jr, Feder JL.SourceDepartment of Biological Sciences, Galvin Life Sciences Building, University of Notre Dame, Notre Dame, IN, USA Department of Biology, Portland State University, OR, USA Department of Entomology, Cornell University, Geneva, NY, USA USDA-ARS, Yakima Agricultural Research Laboratory, Wapato, WA, USA Research and Extension Unit, Washington State University, Vancouver, Vancouver, WA, USA.
Journal of evolutionary biology.J Evol Biol.2012 May;25(5):961-71. doi: 10.1111/j.1420-9101.2012.02489.x. Epub 2012 Mar 21.
Prezygotic isolation due to habitat choice is important to many models of speciation-with-gene-flow. Habitat choice is usually thought to occur through positive preferences of organisms for particular environments. However, avoidance of non-natal environments may also play a role in choice and have
PMID 22435643

Japanese Journal

ROS Production and Apoptosis Induction by Formation of Gts1p-Mediated Protein Aggregates

SANADA Mitsuaki,KURODA Kouichi,UEDA Mitsuyoshi
Bioscience, biotechnology, and biochemistry 75(8), 1546-1553, 2011-08-23
… GTS1 of Saccharomyces cerevisiae is a pleiotropic gene. … Deletion of the genes encoding Por1p and Yhb1p altered the profiles of ROS production and apoptosis caused by GTS1 induction, suggesting that the trapping of these proteins in Gts1p-mediated protein aggregates inhibits the intrinsic functions of these proteins. …
NAID 10029591643

Pathogenic exon-trapping by SVA retrotransposon and rescue in Fukuyama muscular dystrophy

Taniguchi-Ikeda Mariko,Kobayashi Kazuhiro,Kanagawa Motoi,Yu Chih-chieh,Mori Kouhei,Oda Tetsuya,Kuga Atsushi,Kurahashi Hiroki,Akman Hasan O.,DiMauro Salvatore,Kaji Ryuji,Yokota Toshifumi,Takeda Shin'ichi,Toda Tatsushi
NATURE 478(7367), 127-U143, 2011
NAID 120004027028